The number of 5-HT-immunoreactive neurons in the DR was counted using ImageJ 1.51n imaging software in two fields per animal from high magnitude images [x200, DM-200-LED; Leica Microsystems, Heidelberg, Germany; 1.24 ± 0.03 mm2; at bregma 4.6 and −4.8 mm, according to the mouse brain atlas, (Paxinos and Franklin, 2001 )]. The impact of CIH on male and female mice was determined by expressing the number of 5-HT neurons under CIH as the fold change relative to the corresponding sham conditions.
Dm 200 led
Manufactured by Leica
Sourced in Germany
The DM-200-LED is a microscope light source from Leica. It is designed to provide uniform illumination for microscope samples. The DM-200-LED features LED technology for energy-efficient and long-lasting performance.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using dm 200 led
1
Quantifying 5-HT Neurons in Chronic Intermittent Hypoxia
2
Fluorescent Yeast Transformation Imaging
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For the evaluation of yeast transformation, the commercially available fluorescent ligand SNAP-Cell TMR-Star (New England Biolabs) was used.
For image acquisition, an epifluorescence microscope (Leica Microsystems, Germany) equipped with an oil immersion lens (1.4 NA; 100 x; DM200 LED; Leica Microsystems), a N3 filter cube (excitation: 546/12 nm; emission: 600/40 nm) and a GFP filter cube (excitation: 450/90 nm; emission: LP 590 nm) was employed.
For image acquisition, an epifluorescence microscope (Leica Microsystems, Germany) equipped with an oil immersion lens (1.4 NA; 100 x; DM200 LED; Leica Microsystems), a N3 filter cube (excitation: 546/12 nm; emission: 600/40 nm) and a GFP filter cube (excitation: 450/90 nm; emission: LP 590 nm) was employed.
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