Exorbital lacrimal and submandibular salivary glands were fixed in buffered formalin, processed, embedded in paraffin, and sectioned. Five μm sections of paired glands were stained with H&E and inflammation was quantified by light microscopy using standard focus scoring (Barr et al., 2017 (link)). Briefly, slides were analyzed at 10x magnification by a blinded observer to determine the number of mononuclear cell foci in tissue sections of male lacrimal or female salivary glands, with a focus defined as a cluster of at least 50 mononuclear cells. Slides were scanned using PathScan Enabler IV (Meyer Instruments, Houston, TX) to obtain digital images, and tissue areas were measured using ImageJ software (US National Institutes of Health, Bethesda, MD, USA) (Schneider et al., 2012 (link)). Focus scores were calculated as number of foci per 4 mm2 tissue area. Samples with foci that were so numerous that they coalesced were designated as diffuse and assigned focus scores greater than the highest calculable value for that set of comparisons. Representative images were captured on a Leitz DM-RB research microscope with a Leica DCF700T digital camera using the Leica Application Suite X software (Leica Microsystems, Wetzlar, Germany).
Dm rb research microscope
Manufactured by Leica Microsystems
Sourced in Germany
The Leica DM-RB is a research microscope designed for advanced scientific investigations. It features modular components and a robust construction to support a wide range of applications. The DM-RB provides high-quality imaging and precise control for researchers to explore their specimens effectively.
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2 protocols using dm rb research microscope
1
Quantifying Inflammatory Foci in Lacrimal and Salivary Glands
2
Quantifying Inflammatory Foci in Lacrimal and Salivary Glands
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Exorbital lacrimal and submandibular salivary glands were fixed in buffered formalin, processed, embedded in paraffin, and sectioned. Five μm sections of paired glands were stained with H&E and inflammation was quantified by light microscopy using standard focus scoring (Barr et al., 2017 (link)). Briefly, slides were analyzed at 10x magnification by a blinded observer to determine the number of mononuclear cell foci in tissue sections of male lacrimal or female salivary glands, with a focus defined as a cluster of at least 50 mononuclear cells. Slides were scanned using PathScan Enabler IV (Meyer Instruments, Houston, TX) to obtain digital images, and tissue areas were measured using ImageJ software (US National Institutes of Health, Bethesda, MD, USA) (Schneider et al., 2012 (link)). Focus scores were calculated as number of foci per 4 mm2 tissue area. Samples with foci that were so numerous that they coalesced were designated as diffuse and assigned focus scores greater than the highest calculable value for that set of comparisons. Representative images were captured on a Leitz DM-RB research microscope with a Leica DCF700T digital camera using the Leica Application Suite X software (Leica Microsystems, Wetzlar, Germany).
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