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Pacific orange to be distinguished

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The Pacific Orange is a compact and versatile spectrometer designed for a wide range of applications in life science research and analytical laboratories. It offers high-performance optical components, advanced data processing capabilities, and user-friendly software to deliver accurate and reliable spectral analysis.

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Pacific Orange (11 citations)

2 protocols using pacific orange to be distinguished

1

CD8 T Cell Cytotoxicity Assay

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In vitro cytotoxicity assay was performed as previously described (You et al., 2012 ; Zaritskaya et al., 2010 (link)). Briefly, FACS-sorted CD8CD38low and CD8CD38high T cells were resuspended at 5×106/ml in culture medium. Target P815 cells were stained for 30 min with Pacific Orange to be distinguished (1 μg/ml, Thermo Fisher Scientific) and were incubated with CD3 and CD28 antibodies for 30min on ice (5 μg/ml) which allows P815 to activate CD8 T cells. CD8CD38low or CD38high T cells: P815 cells were cocultured for 2.5 hours at the ratio of 1:5. Cytotoxicity was analyzed by measuring the expression of Annexin V and Propidium Iodide stained cells by flow cytometry.
Katsuyama E., Suarez-Fueyo A., Bradley S.J., Mizui M., Marin A.V., Mulki L., Krishfield S., Malavasi F., Yoon J., Ho Sui S.J., Kyttaris V.C, & Tsokos G.C. (2020). The CD38/NAD/SIRTUIN1/EZH2 Axis Mitigates Cytotoxic CD8 T Cell Function and Identifies Patients with SLE Prone to Infections. Cell reports, 30(1), 112-123.e4.
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2

CD8 T Cell Cytotoxicity Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
In vitro cytotoxicity assay was performed as previously described (You et al., 2012 ; Zaritskaya et al., 2010 (link)). Briefly, FACS-sorted CD8CD38low and CD8CD38high T cells were resuspended at 5×106/ml in culture medium. Target P815 cells were stained for 30 min with Pacific Orange to be distinguished (1 μg/ml, Thermo Fisher Scientific) and were incubated with CD3 and CD28 antibodies for 30min on ice (5 μg/ml) which allows P815 to activate CD8 T cells. CD8CD38low or CD38high T cells: P815 cells were cocultured for 2.5 hours at the ratio of 1:5. Cytotoxicity was analyzed by measuring the expression of Annexin V and Propidium Iodide stained cells by flow cytometry.
Katsuyama E., Suarez-Fueyo A., Bradley S.J., Mizui M., Marin A.V., Mulki L., Krishfield S., Malavasi F., Yoon J., Ho Sui S.J., Kyttaris V.C, & Tsokos G.C. (2020). The CD38/NAD/SIRTUIN1/EZH2 Axis Mitigates Cytotoxic CD8 T Cell Function and Identifies Patients with SLE Prone to Infections. Cell reports, 30(1), 112-123.e4.
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