IgE and IgG reactivity to 174 allergen molecules (Table S2 ), was determined, using MeDALL allergen chips, which had been produced according to ImmunoCAP ISAC technology (Thermofisher), as described previously.24 For IgG measurements samples were diluted 1:50 using sample diluent (Thermofisher).
Bound IgE or IgG antibodies, were determined by scanning chips using LuxScan 10K micro‐array Scanner (CapitalBio Technology). The analysis of scanned chips was performed with the micro‐array evaluation software version 3.1.2 (customized for MeDALL chips). Results were summarized in three categories for specific IgE/IgG values (0.1 < ISU < 1, 1 < ISU < 15, and >15 ISAC Standardized Units) (ISU). Patients having antibody levels above 0.1 ISU were considered positive. Subjects displaying IgE reactivity to any of the allergens on the MeDALL chip were considered as having an IgE sensitization.
Bound IgE or IgG antibodies, were determined by scanning chips using LuxScan 10K micro‐array Scanner (CapitalBio Technology). The analysis of scanned chips was performed with the micro‐array evaluation software version 3.1.2 (customized for MeDALL chips). Results were summarized in three categories for specific IgE/IgG values (0.1 < ISU < 1, 1 < ISU < 15, and >15 ISAC Standardized Units) (ISU). Patients having antibody levels above 0.1 ISU were considered positive. Subjects displaying IgE reactivity to any of the allergens on the MeDALL chip were considered as having an IgE sensitization.