The SYBR1 Green PCR Master Mix (Takara, Clontech, Japan) was used to determine the mRNA levels of BAX, BCL-2, MDR-1, and BCRP genes. The analysis of melting curves was performed using real-time PCR system (Rotor Gene 6000, Corbett Life Science). The supplemental table 1 shows the primers used for BAX, BCL-2, MDR-1, MRP, BCRP, β-actin and GAPDH genes. β-actin and GAPDH were used as an internal control, and duplicate analysis was performed for all samples. The list of the primers is given in table 1 .
Sybr1 green pcr master mix
Manufactured by Takara Bio
Sourced in Japan
SYBR1 Green PCR Master Mix is a ready-to-use solution that contains all the necessary components for real-time PCR amplification and detection using the SYBR1 Green dye. It includes a DNA polymerase, dNTPs, buffer, and SYBR1 Green fluorescent dye.
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Lab products found in correlation
2 protocols using sybr1 green pcr master mix
1
Quantitative Real-Time PCR Analysis
2
Quantitative Analysis of Neural Markers
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Total RNAs of cells were extracted using Trizol Reagent (Invitrogen) according to the manufacturer's instruction. Then the RNA samples were used for reverse transcription to get the first strand cDNA by using the PrimeScript1 RT reagent kit (TaKaRa Bio, Dalian, China). The expressions of RhoA and ROCK1 mRNA and neurogenic markers NSE and β III tubulin were quantified using gene specific primers with SYBR1 Green PCRMaster Mix (TaKaRa Bio). GAPDH was used as the endogenous control gene. MiR-142-5p expression was detected using TaqMan MicroRNA Assay Kit (Applied Biosystems), with U6 snRNA used as the endogenous control. All qRT-PCR analysis was performed using an ABI Prism 7500 (Applied Biosystems). The results of qRT-PCR analysis were presented using 2 -ΔΔ CT method.
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