Curcumin, Y15, 15d-PGJ2 and PD68235 were obtained from Sigma-Aldrich (St Louis, MO, USA). U0126 was obtained from Cell Signaling Technology (Danvers, MA, USA). Imatinib and fasudil were obtained from Nanjing EnoGene Biotechnology (Nanjing, China). Rapamycin was obtained from Xi'an Helin Biological Engineering (Xi'an, China). All these compounds were dissolved in dimethylsulfoxide (DMSO; Sinopharm Chemical Reagent Co., Ltd., Shanghai, China) for experiments. Recombinant rat PDGF was obtained from Cell Sciences (Canton, MA, USA). Primary antibodies against VEGF, p-PI3K, PI3K, p-AKT and AKT were obtained from Nanjing EnoGene Biotechnology (Nanjing, China). Primary antibodies against α-smooth muscle actin (α-SMA), α(I) procollagen, fibronectin, p-PDGF-βR, PDGF-βR, p-FAK, FAK, GTP-RhoA and total-RhoA were obtained from Epitomics (San Francisco, CA, USA). Primary antibodies against PPAR-γ, p-ERK and ERK were obtained from Cell Signaling Technology. Primary antibodies against HIF-1α, VEGF-R2, p-mTOR, mTOR, p-p70S6K, p70S6K and β-actin were obtained from Bioworld Technology (Nanjing, China).
P p70s6k
Manufactured by Bioworld Technology
Sourced in China, United States
P-p70S6K is a lab equipment product that measures the phosphorylation of the p70S6 kinase protein. This protein plays a crucial role in cell growth and proliferation pathways.
Automatically generated - may contain errors
Lab products found in correlation
P erk, by Cell Signaling Technology (1 mentions)
Curcumin, by Merck Group (1 mentions)
Fibronectin, by Abcam (1 mentions)
β actin, by Bioworld Technology (1 mentions)
P fak, by Abcam (1 mentions)
15d pgj2, by Merck Group (1 mentions)
P mtor, by Bioworld Technology (1 mentions)
P70s6k, by Bioworld Technology (1 mentions)
Pd68235, by Merck Group (1 mentions)
U0126, by Cell Signaling Technology (1 mentions)
Metformin, by Merck Group (1 mentions)
2 protocols using p p70s6k
1
Molecular Mechanisms of Antifibrotic Compounds
2
Metformin-Mediated AMPK/mTOR Signaling
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Metformin was purchased from Sigma-Aldrich (St. Louis, MO, USA) and dissolved in sterile phosphate-buffered saline (PBS; TransGen Biotech, Inc.,) at a stock concentration of 1 mol/l. The Metformin was stored at −20°C and diluted to the necessary concentration prior to each experiment. The primary antibodies against phosphorylated (p)-AMPKα and AMPKα were purchased from Cell Signaling Technology, Inc. (Boston, MA, USA). Primary antibodies against p-mTOR, mTOR, p-70-kDa ribosomal protein S6 kinase (p70S6K) and p-p70S6K were purchased from Bioworld Technology, Inc. (St. Louis Park, MN, USA). Primary antibodies against β-actin, and horseradish peroxidase-conjugated goat anti-mouse and goat anti-rabbit secondary antibodies were purchased from TransGen Biotech, Inc.
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