N hydroxysuccinimide nhs

The theranostic Hg²⁺ fluorescent probe, 3-formyl-7-diethylamino coumarin masked meso-dimercaptosuccinic acid (C-DMSA), was synthesized according to the literature procedures.11 (link) Chitosan (CS, Mw 50 kDa, 90.0% deacetylation degree) was purchased from JinQiao Biochemical Co. Ltd. (Shandong, China). Chitosan oligosaccharide (CSO, MW 5 kDa, 90.0% deacetylation degree) was obtained from JinQiao Biochemical Co. Ltd. (Shandong, People's
Republic of China). Vitamin E succinate (VES) was purchased from TCI Development Co. Ltd. (Shanghai, People's Republic of China). N-Hydroxysuccinimide (NHS) and 1-Hydroxybenzotriazole (HOBt) were purchased from Adamas Co. Ltd. Mercury perchlorate trihydrate was purchased from XianDing Biotechnology Co. Ltd. (Shanghai, People's Republic of China). All the other analytical chemicals and reagents were analytical grade. FBS, PBS, 0.25% (w/v) trypsin solution, penicillin-streptomycin and DMEM were purchased from Gibco BRL (Gaithersburg, MD, USA). IR-775 chloride, pentobarbital sodium and MTT were purchased from Sigma (St. Louis, USA). Hoechst 33258 was purchased from Beyotime Institute of Biotechnology (Shanghai, People's Republic of China).

Cocoons of Bombyx mori (silkworm) were provided by the College of Textile and Clothing Engineering, Soochow University. Chitosan powders with molecular weight about 900 000 Da and 95% degree of deacetylation were purchased from Jiangsu Aoxin Biotechnology Co., Ltd. Gelatin, sodium carbonate, lithium bromide, acetic acid, aqueous ethanol, polyethylene glycol 6000, dimethylsulfoxide (DMSO), MTT, 4% paraformaldehyde, hematoxylin, eosin, crystal violet, and dialysis bags were obtained from Shanghai Yuanye Biotechnology Co., Ltd. Ethylene dichloride (EDC) and N-Hydroxysuccinimide (NHS) were acquired from Adamas Reagent, Ltd. MEM, FBS, and penicillin–streptomycin were bought from Thermo Fisher Scientific Inc.