Tumor necrosis factor-α (TNF-α), interleukin (IL)-17A, IL-10, IL-6 enzyme-linked immunosorbent assay (ELISA) kits, fluorescent-labeled monoclonal antibodies anti-CD4-fluorescein isothiocyanate (FITC), anti-IgG1-FITC, anti-CD25-Phycoerythrin (PE), anti-IgG1-PE, anti-FoxP3-Allophycocyanin (APC), anti-Interferon (IFN)-gamma-PE, anti-IgG1-APC, and anti-IL-17-APC were bought from eBiosciences (San Jose, CA, USA). FIX & PERM medium was obtained from Invitrogen (California, USA). Leukocyte Activation Cocktail was obtained from BD (New York, USA), Catalog No.550583, containing phorbol 12-myristate 13-acetate (PMA), a calcium ionophore (ionomycin), and the protein transport inhibitor BD GolgiPlug™ (Brefeldin A, New York, USA).
Anti foxp3 allophycocyanin apc
Manufactured by Thermo Fisher Scientific
Sourced in United States
Anti-FoxP3-Allophycocyanin (APC) is a fluorescently labeled antibody that binds to the transcription factor FoxP3. FoxP3 is a key regulator of regulatory T cells (Tregs) and is commonly used as a marker for the identification and analysis of Tregs. The APC fluorescent label allows for the detection and quantification of FoxP3-expressing cells using flow cytometry.
Automatically generated - may contain errors
Lab products found in correlation
Golgiplug, by BD (1 mentions)
Leukocyte activation cocktail, by BD (1 mentions)
Anti il 17 apc, by Thermo Fisher Scientific (1 mentions)
Anti cd4 fluorescein isothiocyanate fitc, by Thermo Fisher Scientific (1 mentions)
Anti cd25 phycoerythrin pe, by Thermo Fisher Scientific (1 mentions)
Anti igg1 pe, by Thermo Fisher Scientific (1 mentions)
Fix perm medium, by Thermo Fisher Scientific (1 mentions)
Facsdiva software, by BD (1 mentions)
Foxp3 transcription factor staining buffer set, by Thermo Fisher Scientific (1 mentions)
Brefeldin a, by Thermo Fisher Scientific (1 mentions)
Ionomycin, by Merck Group (1 mentions)
Monensin, by BioLegend (1 mentions)
4 protocols using anti foxp3 allophycocyanin apc
1
Cytokine and Lymphocyte Profiling Protocol
2
Flow Cytometric Analysis of Regulatory T Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were initially labeled with anti-CD90.1 (thy1.1)-fluorescein isothiocyanate (FITC) (eBioscience, USA) and anti-FOXP3-allophycocyanin (APC) (eBioscience, USA), following the instructions from the manufacturer. Cells were resuspended in 1× PBS and analyzed by flow cytometry. Foxp3 expression was evaluated from CD90.1 gated cells. The samples were analyzed on the FACSCanto II flow cytometer (BD Biosciences, USA), and data analysis was performed with FCS Express 5 Research Edition.
3
Multicolor Flow Cytometry of T Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
T cells were phenotypically analyzed in PBMC by nine-colors polychromatic in flow cytometry in a FACSAria cytometer using FACSDiva software (Becton-Dickinson). For surface staining, 1 million cells PBMC were incubated in four FACS tubes with monoclonal antibodies combinations of fluorescein IsoTioCyanate (FITC)-anti-CCR2/CCR5/CCR6 (Biolegend), peridinin chlorophyll protein (PercP)-anti-CD3 (Biolegend), phycoerythrin-cyanine seven (PE-Cy7)-anti-CD25 (BD), allophycocyanin-alexa-780 (APC-Alexa780)-anti-CCR7 (eBioscience), brilliant violet-405-anti-CD4 (Biolegend), and brilliant violet-605-anti-CD45RA (Biolegend). The cells were fixed and permeabilized with Fix and Perm solution (Anti-Human Foxp3 Set, eBioscience) during 35 min, and then, cells were washed with phosfate saline buffer (PBS) plus FBS (fetal bobine serum), and incubated with Permeabilitation Buffer and Normal Rat Serum (eBioscience) during 15 min. Finally, the cells were incubated 30 min with intracellular monoclonal antibodies: phycoerythrin (PE)-anti-IL-10 (Becton-Dickinson) and allophycocyanin (APC)-anti-FoxP3 (eBioscience).
4
Intracellular Cytokine Profiling of Human Gastric Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For intracellular staining of cytokines, human gastric cells were stimulated with 500 ng/mL phorbol 12,13dibutyrate (PDBU) (Sigma), 1 μM ionomycin (Sigma), and monensin (Biolegend) and brefeldinA (eBioscience) for 5.5 hours. Harvested cells were fixed and permeabilized using Foxp3/Transcription Factor Staining Buffer Set (eBioscience) and stained with anti-human antibodies Phycoerythrin (PE)-anti-IL-5 (BioLegend, clone JES1-39D10), Alexa Fluor (AF) 488=-anti-IL-4 (BioLegend, clone MP4-25D2), Brilliant Violet (BV) 421-anti-IL-13 (BD bioscience, clone JES10-5A2), BV510-anti-IFN-γ (BioLegend, clone 4S.B3), Allophycocyanin (APC)-anti-FOXP3 (eBioscience, clone 236A/e7), BV711-anti-CD3 (Biolegend, clone OKT3), AF700-anti-CD8 (Biolegend, clone HIT8a), APC-Cayanin (Cy)7-anti-CD4 (Biolegend, clone SK3), PE/Dazzle594-anti-IL-10 (Biolegend, clone JES3-19F1), and Peridinin-Chlorophyll-Protein (PercP)-efluor710-anti-GATA3 (eBioscience, clone TWAJ). Samples were resuspended in PBS with 2% FBS and analyzed on a Fortessa cytometer in the CCHMC Research Flow Cytometry Core (RFCC). Single-color compensation was calculated, and data were analyzed in FlowJo (Version 10.6.1). Data analysis was performed on each sample with a minimum number of 1000 live CD3+ cells in the biopsy (3653 ± 2933 CD3 + cells).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!