System 90

Differential scanning calorimetry (DSC) analysis was carried out in a DSC25 Discovery Series (TA Instruments, New Castle, DE, USA) equipped with a cooling system, System 90 (TA Instruments, New Castle, DE, USA). The samples were placed in aluminum crucibles and analyzed in the temperature range between −90 ºC and 200 °C, at heating and cooling rates of 10 °C min⁻¹ under N₂ atmosphere. Three thermal cycles were performed. The melting temperature (T_m, °C) was determined at the minimum of the endothermic peak. The crystallinity (X_c, %) of the samples was estimated as the ratio between melting enthalpy (ΔH_m, J g⁻¹) of its melting peak and the melting enthalpy of a 100% crystalline P(3HB), earlier reported by Morais et al. [45 (link)] and equal to 146 J g⁻¹. Thermogravimetric Analysis (TGA) was performed with a Labsys EVO (Setaram Instrumentation, France). Samples were placed in aluminium crucibles and analyzed in the temperature range between 25 and 500 °C, at 10 °C min⁻¹.

Thermogravimetric Analysis (TGA) was performed using a thermogravimetric equipment Labsys EVO (Setaram Instrumentation, Viroflay, France). Samples were placed in aluminum crucibles and analyzed between 25 and 500 °C, at 10 °C min⁻¹. The thermal degradation temperature (T_deg, °C) corresponds to the temperature value obtained for the maximum decreasing peak of the sample mass. Differential scanning calorimetry (DSC) analysis was done with a differential scanning calorimeter DSC25 Discovery Series (TA Instruments, Wilmington, DE, USA) equipped with a cooling system System 90 (TA Instruments, USA). The samples were placed in aluminum crucibles and analyzed between −90 and 200 °C, with heating and cooling speeds of 10 °C min⁻¹, in N₂ atmosphere (three cycles were performed). The glass transition temperature (T_g, °C) was taken as the midpoint of the heat flux step, and the melting temperature (T_m, °C) was determined at the minimum of the endothermic peak. The crystallinity (X_c, %) was estimated as the ratio between melting enthalpy (ΔH_m, J g⁻¹) associated with the detected melting peak and the melting enthalpy of 100% crystalline P(3HB), estimated as 146 J g⁻¹ [19 (link)].