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Ddpcr cnv assay gapdh

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The DdPCR CNV assay Gapdh is a digital droplet PCR (ddPCR) assay designed to detect and quantify the copy number variation of the Gapdh gene. The assay utilizes the ddPCR technology to partition the sample into thousands of individual droplets, allowing for precise and sensitive measurement of target DNA sequences.

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Lab products found in correlation

Dneasy blood tissue kit, by Qiagen (2 mentions) Qx200 autodg ddpcr system, by Bio-Rad (1 mentions)

Close spelling variants of this product

GAPDH (172 citations) DdPCR assay (8 citations)

2 protocols using ddpcr cnv assay gapdh

1

Quantitative DNA Analysis of Liver Samples

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Genomic DNA from liver samples was extracted using DNeasy Blood & Tissue Kit (QIAGEN; 69506) following the manufacturer's procedures. ddPCR was performed according to the manufacturer's recommendations for the Bio-Rad QX200 AutoDG ddPCR system using 10 or 20 ng of DNA as input and the following probes: Bio-Rad ddPCR CNV assay Gapdh (cat no. 10042961, dMumCNS300520369) and MMUT (cat no. 10042958 dCNS513322846).
Schneller J.L., Lee C.M., Venturoni L.E., Chandler R.J., Li A., Myung S., Cradick T.J., Hurley A.E., Lagor W.R., Bao G, & Venditti C.P. (2021). In vivo genome editing at the albumin locus to treat methylmalonic acidemia. Molecular Therapy. Methods & Clinical Development, 23, 619-632.
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2

Quantitative DNA Analysis of Mouse Tissues

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Brain and liver samples were collected at day 21 of life for both treated and control mice. Genomic DNA from tissue was extracted using DNeasy Blood & Tissue Kit (QIAGEN, Germantown, MD, USA; cat. no. 69506). ddPCR was performed according to the manufacturer’s recommendations for the BioRad QX200 AutoDG ddPCR system using 10 ng or 20 ng of DNA as input and the following probes: Bio-Rad (Hercules, CA, USA) ddPCR CNV assay Gapdh (cat. no. 10042961, dMumCNS300520369) and MMUT (cat. no. 10042958, dCNS513322846).
May F.J., Head P.E., Venturoni L.E., Chandler R.J, & Venditti C.P. (2021). Central nervous system-targeted adeno-associated virus gene therapy in methylmalonic acidemia. Molecular Therapy. Methods & Clinical Development, 21, 765-776.
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