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2 protocols using potassium ferricyanide c6n6fek3

1

Electrochemical Detection of 5-Fluorouracil

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All the chemicals and reagents [thiophene monomer (C4H4S) (≥99% SRL), ferric chloride (FeCl3) (≥98% AR), boron nitride bulk (h-BN) (≥99% SRL), sodium hydroxide (NaOH) (≥98% FISHER), acetonitrile, ethanol, methanol, 5-fluorouracil (C4H3FN2O2) (≥99% SRL), uric acid (C5H4N4O3) (≥99% SRL), lactose (C12H22O11) (≥98% SRL), oxalic acid (≥99.5% AR), ascorbic acid (C6H8O6) (≥99.7% SRL), dopamine (C8H11NO2) (≥99% SRL), l-cysteine (C3H7NO2S) (≥99% SRL), glucose (C6H12O6) (≥99% SRL), potassium ferricyanide (C6N6FeK3) (≥99% SRL), potassium ferrocyanide (C6FeK4N6) (≥99% SRL), potassium chloride (KCl) (99.8% Fisher), monosodium hydrogen phosphate (NaH2PO4) (≥99% SRL), and disodium hydrogen phosphate (Na2HPO4) (≥99% SRL)] are purchased in Sigma Aldrich and Alfa Aesar. To create a stock solution, ultrapure deionized water was used. NaH2PO4 and Na2HPO4 were combined to create 0.1 M phosphate buffer (PBS) (pH = 7.0) for the detection of 5-Fu. The human blood serum sample was collected from SRM Medical College Hospital and Research Center, Kattankulathur, Tamil Nadu, for real sample analysis. All the experiments were done by using Milli-Q-water (18.2 MΩ cm @ 25 ± 2 °C).
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2

Greener Synthesis of Gold Nanoparticles

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Chemicals including 1,1-Diphenyl-2-picryl-hydrazyl (DPPH), methanol, phosphate buffer, potassium ferricyanide (C6N6 FeK3), Trichloroacetic acid (C2HCl3O2), Iron (III) chloride (FeCl3), hydrochloric acid (HCl), nitric acid (HNO3), sodium phosphate, Ammonium molybdate ((NH4)2MoO4), Di-methylsulfoxide (CH3)2SO, and gold (III) chloride trihydrate (HAuCl4·3H2O), silver nitrate (AgNO3) were purchased from Sigma Aldrich, USA. All glassware was rinsed with aqua regia before used for the synthesis of AuNPs. All reagents used were of analytical grade.
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