Human PBMCs were obtained as above, and CD4+ T cells enriched using anti-human-CD4 microbeads (Miltenyi Biotec) before sorting naive CD4+ CD45RA+ CD25−T cells by flow cytometry using an Influx II cell sorter (BD Bioscience, San Diego, CA, USA). Naive T cells were co-cultured with allogenic moDC in StemXvivo (R&D Systems) serum-free medium in roundbottom plates containing interleukin-2 (10 ng ml−1, Biolegend) and anti-CD3 antibody (clone OKT3, 0.2 µg ml−1, Biolegend). In all, 5 × 103 DCs were plated per 1 × 105 T cells in the presence of either 100 µg ml−1 anti-TGFβ (1D11), 20 µg ml−1 anti-integrin β8 (clone 37e1B5,38 100 µg ml−1 isotype control IgG (MOPC-21), or 5 ng ml−1 active TGFβ (Peprotech).
Isotype control igg mopc 21
Manufactured by Thermo Fisher Scientific
Isotype control IgG (MOPC-21) is a monoclonal antibody used in flow cytometry and other immunoassays as a negative control. It is derived from the MOPC-21 mouse myeloma cell line and has an unknown antigen specificity.
Automatically generated - may contain errors
Lab products found in correlation
Anti human cd4 microbeads, by Miltenyi Biotec (2 mentions)
Anti tgfβ, by Thermo Fisher Scientific (2 mentions)
Anti integrin β8 clone 37e1b5 38, by Thermo Fisher Scientific (2 mentions)
Interleukin 2 (il 2), by BioLegend (2 mentions)
Active tgfβ, by Thermo Fisher Scientific (2 mentions)
Influx 2 cell sorter, by BD (2 mentions)
Anti cd3 antibody clone okt3, by BioLegend (2 mentions)
2 protocols using isotype control igg mopc 21
1
Activation of Naive CD4+ T Cells
2
Activation of Naive CD4+ T Cells
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Human PBMCs were obtained as above, and CD4+ T cells enriched using anti-human-CD4 microbeads (Miltenyi Biotec) before sorting naive CD4+ CD45RA+ CD25−T cells by flow cytometry using an Influx II cell sorter (BD Bioscience, San Diego, CA, USA). Naive T cells were co-cultured with allogenic moDC in StemXvivo (R&D Systems) serum-free medium in roundbottom plates containing interleukin-2 (10 ng ml−1, Biolegend) and anti-CD3 antibody (clone OKT3, 0.2 µg ml−1, Biolegend). In all, 5 × 103 DCs were plated per 1 × 105 T cells in the presence of either 100 µg ml−1 anti-TGFβ (1D11), 20 µg ml−1 anti-integrin β8 (clone 37e1B5,38 100 µg ml−1 isotype control IgG (MOPC-21), or 5 ng ml−1 active TGFβ (Peprotech).
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