0.45 μm nylon syringe membranes

The dried stamen or perianth samples (100 mg/sample) were placed in 5 mL quartz tubes equipped with a vapor condenser, then extracted by ultrasound-assisted extraction in 1 mL 90% (v/v) aqEtOH in the USC1200TH ultrasonic bath (Prolabo, Fontenay-sous-Bois, France) following the optimized extraction conditions: 30 kHz frequency at 45 °C for 45 min [33 (link)]. Then, the extract was centrifuged for 15 min at 5000× g (Heraeus Biofuge Stratos, Thermo Scientific, Illkirch, France). The obtained supernatant was filtered using 0.45-μm nylon syringe membranes (Merck Millipore, Saint-Quentin Fallavier, France). Flavonoid enrichment was then performed through the additional DAX-8 (Merck Millipore, Saint-Quentin Fallavier, France) macroporous resin purification step as described in the previously study [33 (link)].

The dried stamen or whole flower samples (100 mg) were placed in 5 mL quartz tubes equipped with a vapor condenser, and then extracted by means of ultrasound-assisted extraction in 1 mL 90% (v/v) aqEtOH in the USC1200TH ultrasonic bath (Prolabo, Fontenay-sous-Bois, France), following optimized extraction conditions: 30 kHz frequency for 45 min at 45 °C [22 (link)]. The extract was centrifuged for 15 min at 5000× g (Heraeus Biofuge Stratos, Thermo Scientific, Illkirch, France), and then the obtained supernatant was filtered through 0.45 μm nylon syringe membranes (Merck Millipore, Saint-Quentin Fallavier, France). The flavonoid enrichment was obtained through the additional DAX-8 (Merck Millipore, Saint-Quentin Fallavier, France) macroporous resin purification step as described in a previous study [22 (link)].