At day 4 post-stimulation, B cells were washed and stained with anti-B220 APC (BioLegend) and either anti-IgG3-FITC (BD-Pharmingen), anti-IgG2b-PE (BioLegend), anti-IgG2a-PE (BioLegend) or anti-IgG1-FITC (BioLegend). Activated B cells from AID-deficient mice were included as negative controls. Data were obtained on 3 × 104 viable cells by using a Coulter XL apparatus (Beckman Coulter).
Anti igg1 fitc
Manufactured by BioLegend
Anti-IgG1-FITC is a fluorescently-labeled antibody that binds to the IgG1 subclass of immunoglobulins. It is used for the detection and quantification of IgG1-expressing cells or molecules in various immunological and flow cytometric applications.
Automatically generated - may contain errors
2 protocols using anti igg1 fitc
1
Activated B cell phenotyping
2
B Cell Isotype Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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At day 4 post-stimulation, cells were washed and stained with anti-B220 APC (BioLegend) and either anti-IgG3-FITC (BD-Pharmingen), anti-IgG1-FITC (BioLegend), anti-IgG2b-PE (BioLegend), anti-IgG2a-PE (BioLegend) or anti-IgA-FITC (BD-Pharmingen). Data were obtained on 3 × 104 viable cells by using a Coulter XL apparatus (Beckman Coulter).
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