After treating, cells were lysed in lysis buffer containing 1% PMSF (Beyotime, Shanghai, China) for 30 min. Equal amounts of proteins were separated, with transferring to a 0.45 µm membrane (GE HealthCare Life Science, Germany) for 1 h at 100 V. Membranes were incubated in 3% nonfat milk for 1 h and then were incubated overnight at 4°C with primary antibodies. Membranes were then incubated with secondary antibodies for 1 h. Immunoreactive bands were visualized by ECL detection reagents.
0.45 m membrane
Manufactured by GE Healthcare
Sourced in Germany
The 0.45 μm membrane is a laboratory filtration product designed for the separation and purification of various liquids and solutions. It is a microporous membrane that retains particles, microorganisms, and other suspended solids larger than 0.45 microns in size, while allowing the passage of smaller molecules and dissolved substances. The membrane is commonly used in a variety of laboratory applications, such as sample preparation, water treatment, and sterile filtration.
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Lab products found in correlation
2 protocols using 0.45 m membrane
1
Western Blot Protein Analysis
2
Western Blot Protein Analysis
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After treating, cells were lysed in lysis buffer containing 1% PMSF (Beyotime, Shanghai, China) for 30 min. Equal amounts of proteins were separated, with transferring to a 0.45 µm membrane (GE HealthCare Life Science, Germany) for 1 h at 100 V. Membranes were incubated in 3% nonfat milk for 1 h and then were incubated overnight at 4°C with primary antibodies. Membranes were then incubated with secondary antibodies for 1 h. Immunoreactive bands were visualized by ECL detection reagents.
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