Total protein from renal cancer samples and cell lines was prepared with ice-cold radioimmunoprecipitation assay buffer (Sigma-Aldrich; Merck Millipore), quanti ed, and loaded onto SDS-PAGE. After electrophoresis, proteins in the gel were transferred to polyvinylidene di uoride membranes (PVDF, Millipore, USA). After blocked for 1 h with 5% nonfat milk, the membranes were incubated overnight at 4°C with primary antibodies, followed with horseradish peroxidase-labeled secondary antibody (sc-2004/sc-2005, Santa Cruz Biotechnology). Signals were detected by chemiluminescence (ECL Western Blotting Detection Reagents, GE Healthcare) and visualized using G:BOX Chemi Gel Documentation System (Frederick, MD, USA). Information on primary antibodies included in this study was listed in Table S2.
G box chemi gel documentation system
Manufactured by GE Healthcare
Sourced in United States
The G:BOX Chemi Gel Documentation System is a laboratory equipment designed for capturing and analyzing images of gels, blots, and other samples. It provides a versatile platform for imaging a wide range of applications in molecular biology and biochemistry.
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2 protocols using g box chemi gel documentation system
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Renal Cancer Protein Quantification
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Renal Cancer Protein Quantification
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Total protein from renal cancer samples and cell lines was prepared with ice-cold radioimmunoprecipitation assay buffer (Sigma-Aldrich; Merck Millipore), quanti ed, and loaded onto SDS-PAGE. After electrophoresis, proteins in the gel were transferred to polyvinylidene di uoride membranes (PVDF, Millipore, USA). After blocked for 1 h with 5% nonfat milk, the membranes were incubated overnight at 4°C with primary antibodies, followed with horseradish peroxidase-labeled secondary antibody (sc-2004/sc-2005, Santa Cruz Biotechnology). Signals were detected by chemiluminescence (ECL Western Blotting Detection Reagents, GE Healthcare) and visualized using G:BOX Chemi Gel Documentation System (Frederick, MD, USA). Information on primary antibodies included in this study was listed in Table S2.
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