The control pLVX-Puro lentivirus and circRNA1615 overexpression lentivirus were constructed by Obio Technology Company (Shanghai, China). The Liposome 3000 reagent was used to transfect the miR-152-3p mimic (5'-UCAGUGCAUGACAGAACUUGG-3'), inhibitor (5'-CCAAGUUCUGUCAUGCACUGA-3') and its negative control. Cells were transfected with control pcDNA3.1 plasmid and LRP6 overexpression plasmid (Generay Technologies, Shanghai, China) using Liposome 3000 reagent. Small interfering RNAs (Shanghai GenePharma Co., Ltd, China) were transfected into the mouse cardiomyocytes using Lipofection 3000 reagent. Empty vector and nonspeci c siRNA (siNC) were used as negative controls. All the sequences of siRNAs were listed in Table 1.
Plvx puro lentivirus
Manufactured by Obio Technology
Sourced in China
The PLVX-Puro lentivirus is a laboratory tool used for gene delivery and gene expression studies. It functions as a vector to deliver genetic material into target cells. The core purpose of this product is to facilitate the introduction of genetic elements into cell lines or primary cells.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using plvx puro lentivirus
1
Lentiviral Transfection of miR-152-3p and LRP6
2
Lentiviral and siRNA Modulation of CircRNA1615 and LRP6
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The control pLVX-Puro lentivirus and circRNA1615 overexpression lentivirus were constructed by Obio Technology Company (Shanghai, China). The Liposome 3000 reagent was used to transfect the miR-152-3p mimic (5′-UCAGUGCAUGACAGAACUUGG-3′), inhibitor (5′-CCAAGUUCUGUCAUGCACUGA-3′) and its negative control. Cells were transfected with control pcDNA3.1 plasmid and LRP6 overexpression plasmid (Generay Technologies, Shanghai, China) using Liposome 3000 reagent. Small interfering RNAs (Shanghai GenePharma Co., Ltd, China) were transfected into the mouse cardiomyocytes using Lipofection 3000 reagent. Empty vector and nonspecific siRNA (siNC) were used as negative controls. All the sequences of siRNAs were listed in table.
siRNA targeting sequence used in the study
| Name | Sense (5′-3′) |
|---|---|
| circRNA1615 | AGUGACUUUAUUUUCCCAU |
| LRP6 | GCGGAUAUCAGACGCAUAU |
| GNAS | CAAUAAUAUGUCAGGACAA |
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!