Soybean-phosphatidylcholine (PC, 95%) was purchased from Avanti Polar Lipids (Alabaster, AL, USA). Tributyrin (TC4, 99%), Tricaprylin (TC8, 99%), Triolein (TC18, 99%), 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC, ≥97%), l -α-phosphatidylinositol (PI, ≥99%), 1,2-diacyl-sn-glycero-3-phospho-l -serine (PS, ≥97%), l -α-phosphatidylethanolamine (PE, ≥99%), cardiolipin (CL, ≥98%), 3-sn-Phosphatidic acid sodium salt (PA, ≥98%), Sphingomyelin (SM, ≥98%), 1,2-dioleoyl-sn-glycero-3-phospho-rac-(1-glycerol) sodium salt (PG, ≥98%), 1,2-distearoyimonoglactosylglyceride (MGDG, ≥98%) were all from Sigma-Aldrich Corporation (St. Louis, MO, USA). Olive oil and sucrose esters were purchased from Aladdin Reagent Co. (Shanghai, China). Six pure chiral pseudoglycerides (didecanoyl-deoxyamino-O-methyl glycerol, DDGs) used in the present studies were synthesized according to the method reported before [23 (link)].
Soybean phosphatidylcholine
Manufactured by Avanti Polar Lipids
Sourced in United States
Soybean phosphatidylcholine is a naturally occurring phospholipid derived from soybeans. It is a key component of cell membranes and plays a crucial role in various biological processes.
Automatically generated - may contain errors
Lab products found in correlation
1 2 dioleoyl sn glycero 3 phospho rac 1 glycerol sodium salt, by Merck Group (1 mentions)
1 2 dioleoyl sn glycero 3 phosphocholine, by Merck Group (1 mentions)
Cardiolipin, by Merck Group (1 mentions)
Tributyrin, by Merck Group (1 mentions)
Sphingomyelin, by Merck Group (1 mentions)
Tricaprylin, by Merck Group (1 mentions)
1 2 diacyl sn glycero 3 phospho l serine, by Merck Group (1 mentions)
L α phosphatidylinositol, by Merck Group (1 mentions)
L α phosphatidylethanolamine, by Merck Group (1 mentions)
Triolein, by Merck Group (1 mentions)
Milli q system, by Merck Group (1 mentions)
Acetonitrile, by Avantor (1 mentions)
Cholesterol, by Avanti Polar Lipids (1 mentions)
Alpha tocopherol, by Merck Group (1 mentions)
Sabouraud s dextrose agar, by Merck Group (1 mentions)
Restriction endonuclease, by New England Biolabs (1 mentions)
3 3 cholamidopropyl dimethylammonio 1 propane sulfonate, by Merck Group (1 mentions)
Dna modifying enzymes, by New England Biolabs (1 mentions)
L α lysophosphatidylcholine, by Merck Group (1 mentions)
4 protocols using soybean phosphatidylcholine
1
Lipid Synthesis and Characterization
2
Quantification of Voriconazole and Metabolite
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Cholesterol and soybean phosphatidylcholine (PC) were purchased from Avanti Polar Lipids (Alabaster, AL, USA). Voriconazole (VOR) was purchased from Hangzhou Dayangchem Co., Limited (Hangzhou, China). and voriconazole-N-oxide (VNO) was purchased from Genone Biotechnologies (Rio de Janeiro, RJ, Brazil) Alpha-tocopherol and Sabouraud’s dextrose agar were purchased from Sigma Aldrich (St. Louis, MO, USA). Acetonitrile was purchased from J. T. Baker (Phillipsburg, NJ, USA). Water was purified using a Milli-Q system (Millipore, Billerica, MA, USA) with a 0.22 µm pore end-filter. All other chemicals and reagents were of analytical grade or superior.
3
Enzyme Isolation and Lipid Preparation
4
Immobilized Phospholipase A1 Catalysis
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Phospholipase A 1 (PLA 1 ) Lecitase ® Ultra, a kind donation by NOVO (Salem, VA) , was immobilized on Duolite A568, a gift from Rohm & Haas (Philadelphia, PA) , according to the method described previously 26) . Soybean phosphatidylcholine (PC, 95％) , was obtained from Avanti Polar Lipids, Inc. (Alabaster, AL) , and HPLC standards of dioctanoyl-L-α-phosphatidylcholine (99％) and L-α-Lysophosphatidylcholine (LPC, 98％) , were purchased from SIGMA-AL-DRICH ® (México City) . A mixture of free medium chain fatty acid (MCFA) , composed of caproic-C6:0 (1.65 mol％) , caprylic-C8:0 (67.14 mol％) , capric-C10:0 (30.57 mol％) , and lauric-C12:0 (0.64 mol％) acids, was obtained by saponifying medium chain oil (Original Thin Oil ® ) purchased from Sound Nutrition (Dover, ID) , according to the method described previously 32) . HPLC grade solvents were purchased from Tecsiquim (Mexico City) and all other reagents used were of analytical grade from SIGMA-ALDRICH ® (Mexico City) .
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