Su dhl 6

RI-1, also known as RIVA, U2932, NU-DHL-1 cell lines were purchased from DSMZ. SU-DHL-4, SU-DHL-6 were purchased from ATCC. All lines were cultured in RPMI 1640 with L-Glutamine (ThermoFisher),10% fetal bovine serum (Thermofisher), at 37 degrees Celsius, 5% CO₂. OCI-LY10 cells, obtained from University Health Network, Ontario, Canada, were cultured in Iscove’s Modified Dulbecco’s medium (Thermofisher) with 20% human serum (Valley Biomedical). IMPACT III testing (IDEXX Bioresearch) was carried out on cell lines to confirm mycoplasma- and pathogen-free status. Cell lines were expanded and cryopreserved following 2–3 passages after obtaining from vendor. After thawing, cells were maintained (for experiments, xenografting) for a maximum of 8 weeks by sub-culturing approximately 3 times a week.

HEK293T (ATCC), OC1-LY7 (ATCC), SUDHL6, OC1-LY1, DoHH2, Karpas422, A20 (Barts Cancer Institute), human EBV (Hernández-Ramírez et al., 2016 (link))

HBL-1, TMD8, OCI-LY7 and SU-DHL-16 cell lines were gifts from Dr. Fu, University of Nebraska Medical Center (Omaha, NE, USA). SU-DHL-2, SU-DHL-6, Pfeiffer, OCI-LY10, OCI-LY8, KARPAS-422 and U2932 cell lines were obtained from ATCC (Manassas, VA, USA) and DSMZ (Braunschweig, Germany). Cells were cultured in IMDM, DMEM or RPMI 1640 medium (Gibco, Life Technologies, Carlsbad, CA, USA) supplemented with 10–20% fetal bovine serum (FBS; Gibco, Life Technology) and penicillin-streptomycin in a humidified atmosphere of 5% CO₂ at 37 °C.

Human MCL cell lines Jeko‐1 and Mino were a kind gift from Dr Jianguo Tao at the H. Lee Moffitt Cancer Center & Research Institute. The HBL‐2 cell line was a kind gift from Dr Elliot Epner at Oregon Health and Science University. We purchased SUDHL‐6 (CRL‐2959™), DG‐75 (CRL‐2625™) and HEK293 (CRL‐1573™) from ATCC. All cell lines were maintained in RPMI medium 1640 with GlutaMAX (ThermoFisher Scientific), supplemented with 10% foetal bovine serum (ThermoFisher Scientific), and treated with 100 U/mL penicillin and 100 μg/mL streptomycin (both ThermoFisher Scientific) in a humidified atmosphere containing 5% CO₂ at 37°C. The treatments of the cells by deferoxamine mesylate salt (250 µmol/L, DFO, Sigma‐Aldrich) and dimethyloxalylglycine (1 mmol/L, DMOG, Sigma‐Aldrich) are indicated in the corresponding figures and legends. For hypoxia induction, cells were cultured 24 hours in hypoxia chamber (StemCell Technologies) containing certified gases mixture (1% O₂, 5% CO₂, 94% N₂), which was placed in the standard tissue culture incubator at 37°C. Cultures and assays used for analyses of mouse embryonic stem cells (mESCs) are described in Appendix S1.