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Pnfkb

Manufactured by Merck Group
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PNFkB is a lab equipment product. It is a tool used for the detection and analysis of the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) signaling pathway. The core function of PNFkB is to facilitate the study of NF-κB, a transcription factor that plays a crucial role in cellular processes, such as inflammation, immune response, and cell survival.

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Lab products found in correlation

Pstat3, by Merck Group (2 mentions) Nf kb, by Cell Signaling Technology (2 mentions) Pnfkb, by Cell Signaling Technology (2 mentions) P atf2, by Cell Signaling Technology (2 mentions) Pc jun, by Merck Group (2 mentions) Patf 2, by Merck Group (2 mentions) Protease inhibitor and phosphatase inhibitor cocktail 2 3, by Merck Group (2 mentions) P stat3, by Cell Signaling Technology (2 mentions) Protein a g plus agarose, by Santa Cruz Biotechnology (2 mentions) P c jun, by Cell Signaling Technology (2 mentions)

2 protocols using pnfkb

1

Investigating Transcription Factor Activation

Check if the same lab product or an alternative is used in the 5 most similar protocols
LECs (2×106) treated with Stattic, SP600125, IL6 or EGM, were used to prepare cell lysates or nuclear extracts. Five hundred microliters of cell lysates or 200 µL nuclear extracts were incubated overnight at 4°C with antibodies suitable for IP (1:100 diluted): pStat3, pc-Jun, pATF-2, pNFkB, and NFkB (Cell Signaling). Ten microliters of Protein A/G Plus Agarose (Santa Cruz Biotech) was added and incubated for 3 h at 4°C. The beads were rinsed 3 times with 500 µL cell lysis buffer for IP (Pierce) supplemented with the protease inhibitor and phosphatase inhibitor cocktail 2/3 (Sigma). The protein complex was reduced and separated by SDS-PAGE and probed with the following antibodies in a Western assay: pStat3, pc-Jun, pATF-2, pNFkB, and NFkB (Sigma). All the original gel images of immunoblot analyses are presented in Supplementary Fig. 25.
Lee E., Fertig E.J., Jin K., Sukumar S., Pandey N.B, & Popel A.S. (2014). Breast cancer cells condition lymphatic endothelial cells within pre-metastatic niches to promote metastasis. Nature communications, 5, 4715.
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2

Investigating Transcription Factor Activation

Check if the same lab product or an alternative is used in the 5 most similar protocols
LECs (2×106) treated with Stattic, SP600125, IL6 or EGM, were used to prepare cell lysates or nuclear extracts. Five hundred microliters of cell lysates or 200 µL nuclear extracts were incubated overnight at 4°C with antibodies suitable for IP (1:100 diluted): pStat3, pc-Jun, pATF-2, pNFkB, and NFkB (Cell Signaling). Ten microliters of Protein A/G Plus Agarose (Santa Cruz Biotech) was added and incubated for 3 h at 4°C. The beads were rinsed 3 times with 500 µL cell lysis buffer for IP (Pierce) supplemented with the protease inhibitor and phosphatase inhibitor cocktail 2/3 (Sigma). The protein complex was reduced and separated by SDS-PAGE and probed with the following antibodies in a Western assay: pStat3, pc-Jun, pATF-2, pNFkB, and NFkB (Sigma). All the original gel images of immunoblot analyses are presented in Supplementary Fig. 25.
Lee E., Fertig E.J., Jin K., Sukumar S., Pandey N.B, & Popel A.S. (2014). Breast cancer cells condition lymphatic endothelial cells within pre-metastatic niches to promote metastasis. Nature communications, 5, 4715.
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