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M205c stereomicroscope

Manufactured by Olympus
Sourced in Canada

The M205C stereomicroscope is a high-quality optical instrument designed for detailed observation and analysis. It features a zoom range of 6.3x to 40x, providing users with a versatile magnification capability. The M205C utilizes a Greenough optical system, delivering a clear, detailed image with minimal distortion. This microscope is suitable for a variety of applications that require precise visual inspection and examination.

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7 protocols using m205c stereomicroscope

1

Spider Morphology Imaging Protocol

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Genitalic structures (epigyna, male palps) and habitus for each putative morphological species were digitally imaged. Male palps and epigyna were examined and photographed with Leica M205C stereomicroscope and Olympus BX51 compound microscope after being dissected from the spider bodies. The digital images depicting the habitus and genital morphology were a composite of multiple images taken at different focal planes along the Z axis and assembled using the software package Helicon Focus 3.10. Left palps were photographed unless otherwise stated. Genitalia were cleared in boiling 10% KOH for a few minutes to dissolve soft tissues.
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2

Detailed Microscopic Examination of Spider Genitalia

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Specimens were examined with an Olympus SZX16 stereomicroscope; details were further investigated with an Olympus BX51 compound microscope. Epigyne were cleared in proteinase K at 56 C to dissolve non-chitinous tissues. Photos were taken with Leica M205C stereomicroscope and Olympus BX51 equipped with a Micropublisher 3.3 RTV camera (QImaging, Surrey, BC, Canada). The digital images depicting the habitus and genital morphology were a composite of multiple images taken at different focal planes along the Z axis and assembled using the software package Helicon Focus 3.10.
Leg measurements are shown as: total length (femur, patella, tibia, metatarsus, tarsus). Numbers of spines are listed for each segment in the following order: prolateral, dorsal, retrolateral, ventral (in femora and patellae ventral spines are absent and fourth digit is omitted in the spination formula). All measurements are in millimeters.
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3

Detailed Spider Morphometric Analysis

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Specimens were examined and measured with a Leica M205 C stereomicroscope; details were studied with an Olympus BX51 compound microscope. Male and female copulatory organs were examined and illustrated after they were dissected from the spiders. Epigynes were removed and treated in 10% warm solution of potassium hyPageBreakdroxide (KOH) before illustration. Type specimens were preserved in 75% ethanol solution. Photographs were taken with an Olympus C7070 wide zoom digital camera (7.1 megapixels) mounted on a Leica M205 C stereomicroscope. The images were assembled using Helicon Focus 3.10 image stacking software. All measurements are given in millimeters unless noted otherwise. Leg measurements are shown as: Total length (femur + patella + tibia + metatarsus + tarsus). Leg segments were measured on their dorsal side. Type specimens are deposited in the Thailand Natural History Museum, Pathum Thani, Thailand.
Terminology and taxonomic descriptions follow Huber (2000) . The following abbreviations are used in the descriptions: ALE

= anterior lateral eye

, AME

= anterior median eye

, PME

= posterior median eye

, L/d

= length/diameter

.
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4

Detailed Morphological Examination of Spider Specimens

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Types are deposited in the Institute of Zoology, Chinese Academy of Sciences (IZCAS) in Beijing. All specimens collected were preserved and observed in a 95% ethanol solution. The specimens were measured and examined under a Leica M205C stereomicroscope, and further morphological details were observed using an Olympus BX41 compound microscope. The male palp was dissected from the left side of the spider for further examination. The carapace measurements include the clypeus (except for Relictocera sp. which has a distinct clypeus). The length and width ratios were measured according to the length of the cymbium (including the cymbial protrusion) to its width. The internal genitalia of the female and the male palp were dissected and immersed in lactic acid for digestion. An Olympus C7070 wide zoom digital camera (7.1 megapixels) mounted on an Olympus SZX12 stereomicroscope was used to take photos at different focal plans. The photos were assembled with the image stacking software Helicon Focus 6.7.1 to generate high quality photos before further editing with Adobe Photoshop CC 2014. Leg measurements are given as total length (femur, patella, tibia, metatarsus, and tarsus). Leg segments were measured from their retrolateral side. All measurements are given in millimetres (mm). Terminology follows Li et al. (2014) (link), Tong and Li (2007) , and Deeleman-Reinhold (1995) .
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5

Morphological Examination of Spiders

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Types are deposited in the Institute of Zoology, Chinese Academy of Sciences (IZCAS) in Beijing. All specimens collected were studied and preserved in 95% ethanol. The specimens were measured and examined with a Leica M205 C stereomicroscope, and further morphological details were observed with an Olympus BX41 compound microscope. Male palps were detached from the left side of the animal for further examination (except for Leclerceraxiangbabang sp. nov. whose right palp was detached). Carapace length was measured excluding the clypeus. Internal genitalia of the female and palpal bulbs were dissected and immersed in lactic acid. An Olympus C7070 wide zoom digital camera (7.1 megapixels) mounted on an Olympus SZX12 stereomicroscope was used to take photos at different focal planes. The photos were then transferred to the image stacking software Helicon Focus 6.7.1 to generate photos with a greater depth of field before further processing with Adobe Photoshop CC 2014. Leg measurements are shown as total length: femur, patella, tibia, metatarsus, and tarsus. Leg segments were measured from their retrolateral side. All measurements are given in millimetres (mm). All terminology follows that of Li et al. (2014) (link).
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6

Detailed Morphological Examination of Specimens

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Types are deposited in the Institute of Zoology, Chinese Academy of Sciences (IZCAS) in Beijing. All specimens collected were studied and preserved in 75% ethanol. The specimens were measured and examined with a Leica M205 C stereomicroscope and further morphological details were observed with an Olympus BX41 compound microscope. Male palps were detached from the left side of the animal for further examination. Carapace length was measured excluding the clypeus. Internal genitalia of the female and palpal bulbs were dissected and immersed in lactic acid. An Olympus C7070 wide zoom digital camera (7.1 megapixels) mounted on an Olympus SZX12 stereomicroscope was used to take photos at different focal planes. The photos were then transferred to the image stacking software Helicon Focus 6.7.1 to generate photos with a greater depth of field before further processing with Adobe Photoshop CC 2014. Leg measurements are shown as total length: femur, patella, tibia, metatarsus and tarsus. Leg segments were measured from their retrolateral side. All measurements are given in millimetres (mm). All terminology follows that of Li et al (2014) (link). The distribution map was generated with Google Earth Pro 7.3.2 (Google Limited Liability Company).
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7

Stereomicroscopic Examination of Beetle Genitalia

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Specimens were examined with a Leica M205c stereomicroscope and an Olympus BX51 compound microscope. Genitalia were drawn with the aid of a drawing tube. Male genitalia were placed in concentrated lactic acid in a cavity slide for at least several hours before they were examined. Habitus photographs were made with a KEYENCE VHX-2000 – Super Resolution Digital Microscope System. Label data are cited verbatim, with separate lines on the same label indicated by a slash “/”; different labels are separated by a vertical line“|”.
Abbreviations used in the text:
BL body length = PL+EL,
BW maximum width of body,
PL
pronotal length,
PW maximum width of pronotum.
EL elytral length,
EW maximum width of elytra,
CWBS
China Water Beetle Survey,
The type specimens of the new species are deposited in the Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, China (IAECAS) and in the Natural History Museum Vienna, Austria (NMW).
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