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Anti cd4 pe cy5

Manufactured by Thermo Fisher Scientific

The Anti-CD4-PE-Cy5.5 is a fluorescent-labeled antibody that binds to the CD4 cell surface antigen. It is designed for use in flow cytometry applications to identify and quantify CD4-positive cells within a sample.

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2 protocols using anti cd4 pe cy5

1

Quantifying CD38 Expression on CD8+ T Cells During HIV-1 Infection

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As a measure of immune activation, the expression of CD38 on the surface of CD8+ T cells was quantified over the first year of HIV-1 infection. Briefly, PBMCs were stained with anti-CD3-Pacific Blue, anti-CD8-Alexa 700, anti-CD38-PerCP (BD Biosciences), and anti-CD4-PE-Cy5.5 (Invitrogen). The cells were then washed, resuspended in 2% paraformaldehyde, acquired using a BD LSRII flow cytometer, and analyzed with FlowJo software (Treestar).
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2

Multiparameter Flow Cytometry Immunoassay

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Antibodies used for surface staining included anti-CD14 Qdot 655, anti-CD20 Qdot 655, anti-CD4 PeCy5.5, anti-CD8 Qdot605 (Invitrogen; Carlsbad, California), anti-CD14 BV650, anti-CD20 BV650 (Biolegend; San Diego, California), anti-CD28 ECD (Beckman Coulter; Fullerton, CA), anti-CD25 APC-Cy7, anti-CD95 PE-Cy5, and anti-HLA-DR APC (BD Pharmingen; San Diego, California). Antibodies used for intracellular staining included anti-interleukin-2 (IL-2) Alexa700 (Biolegend), anti-interferon-gamma (IFNγ) Alexa 700 (Invitrogen), anti-tumor necrosis factor-alpha (TNFα) Alexa 700, anti-CD3 Pac Blue, anti-CD69 PE, and anti-Ki67 FITC (BD Pharmingen).
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