Cdk5 c 8

Manufactured by Santa Cruz Biotechnology

Sourced in Canada

Cdk5 (C-8) is a monoclonal antibody that recognizes the Cdk5 protein. Cdk5 is a serine/threonine protein kinase involved in the regulation of various cellular processes. The antibody can be used for the detection of Cdk5 in various applications such as Western blotting, immunoprecipitation, and immunohistochemistry.

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Lab products found in correlation

Triton x 100, by Merck Group (1 mentions) Lsm 700 microscope, by Zeiss (1 mentions) Alexa 488, by Thermo Fisher Scientific (1 mentions) Alexa 647, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Rhodamine phalloidin, by Thermo Fisher Scientific (1 mentions) F12 medium, by Thermo Fisher Scientific (1 mentions) Poly d lysine laminin, by Merck Group (1 mentions) Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions) Paraformaldehyde (pfa), by Electron Microscopy Sciences (1 mentions) Fetal bovine serum (fbs), by Cytiva (1 mentions) Phospho akt, by Cell Signaling Technology (1 mentions) Phospho gsk 3β ser9, by Cell Signaling Technology (1 mentions) Sapk jnk, by Cell Signaling Technology (1 mentions) Phospho sapk jnk, by Cell Signaling Technology (1 mentions) P38 mapk, by Cell Signaling Technology (1 mentions) Pp2a c, by Cell Signaling Technology (1 mentions) Phospho p44 42 mapk, by Cell Signaling Technology (1 mentions) Total camkii, by Santa Cruz Biotechnology (1 mentions) Gsk3β, by BD (1 mentions)

2 protocols using cdk5 c 8

Trigeminal Ganglia Neuron Immunofluorescence

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Trigeminal ganglia (TG) neurons were plated onto Lab-Tek 8 chamber cover-slides (Thermo Fisher Scientific, Waltham, CA) coated with laminin/poly-D-lysine (Sigma-Aldrich) with F12 medium (Thermo Fisher Scientific) containing 10% FBS (HyClone Laboratories, Logan, UT) and 1% penicillin/streptomycin (Thermo Fisher Scientific). The following day, cultured cells were fixed in 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA) and permeabilized for 10 minutes with 0.1% Triton X-100 (Sigma-Aldrich). Cells were probed with the following primary antibodies: TRPA1 (Novus Biologicals LLC, Littleton, CO), p35 (C-19) and Cdk5 (C-8) (both Santa Cruz Biotechnology). After several washes with PBS (Thermo Fisher Scientific), cultured neurons were incubated with secondary antibodies conjugated with either Alexa 488 or Alexa 647 (1:500, Molecular Probes, Carlsbad, CA) and finally labeled with rhodamine-phalloidin (1:300, Molecular Probes, Carlsbad, CA) for 20 minutes. All the images were acquired using a Zeiss LSM 700 microscope.

Hall B.E., Prochazkova M., Sapio M.R., Minetos P., Kurochkina N., Binukumar B.K., Amin N.D., Terse A., Joseph J., Raithel S.J., Mannes A.J., Pant H.C., Chung M.K., Iadarola M.J, & Kulkarni A.B. (2018). Phosphorylation of the Transient Receptor Potential Ankyrin 1 by Cyclin-dependent Kinase 5 affects Chemo-nociception. Scientific Reports, 8, 1177.

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Tau Kinase and Phosphatase Expression Profiling

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The changes in the expression and activation of tau kinases were examined using the following antibodies: GSK-3β (1:1000, BD Transduction Lab, Franklin Lakes, NJ) as well as the following antibodies purchased from Cell Signaling Technology, Inc. (Danvers, MA): phospho-GSK-3β (Ser9, 1:1000), p35/p25 (1:1000), phospho-Akt (Ser⁴⁷³, 1:1000), Akt (1:1000), SAPK/JNK (1:1000), phospho-SAPK-JNK (Thr¹⁸³/Tyr¹⁸⁵, 1:1000), p44/42 MAPK (Erk 1/2, 1:1000), phospho-p44/42 MAPK (Erk 1/2, Thr²⁰²/Tyr²⁰⁴, 1:1000), p38 MAPK (P38, 1:1000). Cdk5 (C-8), phospho-Ca²⁺/calmodulin-dependent protein kinases II (CaMKII, Thr²⁸⁶, 1:1000) and total CaMKII (1:1000) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA). Phospho-p38 (clone 6E5.2, p-P38, Thr¹⁸⁰/Tyr¹⁸², 1/1000) was purchased from Millipore, and GSK-3 α/β PY279/PY216 (Tyr²⁷⁹/Tyr²¹⁶, 1:1000) from Life Technologies (Grand Island, NY).
The changes in the expression of the tau phosphatases were determined using antibodies directed at PP2A-C (1:1000, Cell Signaling), PP5 (1:1000, Cell Signaling), PP2A-Bα subunit (2G9), 1:1000, Cell Signaling), demethylated PP2A-C subunit (4B7, 1:1000, Santa Cruz), PP2A-A subunit (1:1000, Cell Signaling), pan-calcineurin A (PP2B, 1:1000, Cell Signaling), and the PP1 catalytic subunit (E-9, 1:1000, Santa Cruz).

Whittington R.A., Virág L., Gratuze M., Petry F.R., Noël A., Poitras I., Truchetti G., Marcouiller F., Papon M.A., Khoury N.E., Wong K., Bretteville A., Morin F, & Planel E. (2015). Dexmedetomidine Increases Tau Phosphorylation Under Normothermic Conditions In Vivo and In Vitro. Neurobiology of aging, 36(8), 2414-2428.

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