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Corn oil c8267 500ml

Manufactured by Merck Group

Corn oil (C8267-500ML) is a laboratory reagent provided by Merck Group. It is a clear, pale yellow liquid extracted from the germ of corn kernels. The product serves as a general-purpose oil for various laboratory applications.

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2 protocols using corn oil c8267 500ml

1

Formulation and Characterization of Cy5-PLGA Nanoparticles

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PLGA (50:50, AP041, lot: 200825RAI‐B, MW 10–15 kDa) and Cy5‐labeled PLGA (50:50 PLGA‐Cy5, AV034, lot: 201215RAI‐A, MW 10–15 kDa) were purchased from Akina. All‐trans retinoic acid (BML‐GR100‐0500, lot: 06011830) was purchased from Enzo. Dimethyl sulfoxide (DMSO, D128‐500, lot: 194474) and dichloromethane (DCM, D143‐1, lot: 194105) were purchased from Fischer Chemical. Poly‐vinyl alcohol (363146‐500G, lot: MKCF9787, MW 85–124 kDa), corn oil (C8267‐500ML, lot: MKCM9808), and Mannan (M7504‐5G, lot: SLCF4977) were purchased from Sigma‐Aldrich. Roswell Park Memorial Institute (RPMI) powder was purchased from (Gibco, lot: 2344357). Mannan was purchased from Sigma‐Aldrich. Incomplete Freund's Adjuvant + Ovalbumin (EK‐0311, lots: 105, 111) and Complete Freund's Adjuvant + Ovalbumin (EK‐0301, lots: 105, 111) were purchased from Hooke Labs.
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2

Oleyl alcohol and oil supplementation in cell culture

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Cells were cultivated as described above for the fed-batch experiments. A reduced media volume of 4.5 mL was supplemented with 10 % or 20 % oleyl alcohol, sunflower oil or corn oil in 250-mL bottles to avoid the need for gas renewal during experiments without growth curves. Bottles were inoculated from exponential pre-cultures to OD600 = 0.1–0.2. Sunflower seed oil from Helianthus annuus (88921-250ML-F) and corn oil (C8267-500ML) were both purchased from Sigma-Aldrich Chemie, filter-sterilized and stored under anoxic conditions for several days before use.
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