Rabbit anti insulin receptor β

The following antibodies were used for immunoblotting: Rat anti-HA (Roche, ROAHAHA), Rabbit anti- FLAG (Proteintech, 20543-1-AP), Mouse anti-GAPDH (Developmental Studies Hybridoma Bank, hGAPDH-2G7), Rabbit anti-USP46 (Proteintech, 13502-1-AP), Mouse anti-Tubulin (Developmental Studies Hybridoma Bank, E7), Mouse anti-β-catenin (BD Transduction Laboratory, 610154), Rabbit anti-Axin1 (Cell Signaling Technology, 2087), Rabbit anti-Insulin Receptor β (Cell Signaling Technology, 3025), Rabbit anti-LRP6 (Cell Signaling Technology, 2560), Rabbit anti-WDR20 (Bethyl Laboratories, A301-657A), Rabbit anti-WDR48 (UAF1) (Proteintech, 16503-1-AP), Goat anti-rat IgG H + L-HRP (Thermo, 31470), Goat anti-mouse IgG H + L-HRP (Promega, W4021), Goat anti-rabbit IgG H + L-HRP (Promega, W4011). All primary antibodies were used at 1:1000 dilution except anti-FLAG (1:2000), anti-GAPDH (1:500), and anti-WDR20 (1:2500). All secondary HRP antibodies were used at 1:5000 dilution.

Isolated primary microglial samples (see above) were used for evaluation of knock-out efficiency by measuring InsR protein concentration, following the established protocol (see supplementary methods).
Primary antibodies: rabbit anti-insulin receptor β (Ref: 3025, Cell Signaling), goat anti-actin (Ref: sc-1616, Santa Cruz). Secondary antibodies: rabbit anti-goat immunoglobulins/HRP (Ref: P0449, Dako), goat anti-rabbit immunoglobulins/HRP (Ref: P0448, Dako).
Data presented consists of a comparison between the gray area in InsR-KO and InsR-Ctrl samples in ImageJ [47 (link)].