Msp anchorchip target plate

Manufactured by Bruker

The MSP AnchorChip target plate is a lab equipment product designed for use in matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. The product provides a platform for sample preparation and analysis.

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Lab products found in correlation

Peptide calibration standard 2, by Bruker (3 mentions) Flexanalysis software, by Bruker (3 mentions) Microflex maldi tof mass spectrometer, by Bruker (2 mentions)

3 protocols using msp anchorchip target plate

MALDI-TOF Analysis of Peptide Conjugation

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Conjugation efficiency was determined using MALDI-TOF. Peptides were added to a saturated solution of α-cyano-4-hydroxycinnamic acid in 70% methanol, 0.05% TFA and dried onto the MSP AnchorChip target plate (Bruker) using the dried droplet method. The Bruker Microflex MALDI-TOF mass spectrometer was calibrated with the Bruker Peptide Calibration Standard II. Mass spectra were analyzed using FlexAnalysis software (https://researchservices.pitt.edu/sites/default/files/flexAnalysis%20User%20Manual.pdf) (Bruker).

Rybak J.A., Sahoo A.R., Kim S., Pyron R.J., Pitts S.B., Guleryuz S., Smith A.W., Buck M, & Barrera F.N. (2023). Allosteric inhibition of the epidermal growth factor receptor through disruption of transmembrane interactions. The Journal of Biological Chemistry, 299(7), 104914.

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MALDI-TOF Analysis of Peptides

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Peptides were added to a saturated solution of α-HCCA in 70% methanol with 0.05% TFA. The resulting solution was dried onto the MSP AnchorChip target plate (Bruker, Billerica, MA) using the dried droplet method (Karas and Hillenkamp, 1988 (link)). The Bruker Microflex MALDI-TOF mass spetrometer was calibrated with the Bruker Peptide Calibration Standard II (Billerica, MA). Mass spectra were analyzed using FlexAnalysis software (Bruker, Billerica, MA).

Alves D.S., Westerfield J.M., Shi X., Nguyen V.P., Stefanski K.M., Booth K.R., Kim S., Morrell-Falvey J., Wang B.C., Abel S.M., Smith A.W, & Barrera F.N. (2018). A novel pH-dependent membrane peptide that binds to EphA2 and inhibits cell migration. eLife, 7, e36645.

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MALDI-TOF Mass Spectrometry of Peptides

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For matrix-assisted laser desorption ionization time of flight (MADLI-TOF) mass spectrometry measurements, peptide in solvent was mixed with a saturated solution of a-cyano-4-hydroxycinnamic acid (a-HCCA) in a mixture of 70% methanol and 0.05% trifluoroacetic acid. This solution was dried dropwise onto an MSP AnchorChip target plate (Bruker, Billerica, MA). Mass spectra were collected on a Bruker Microflex MALDI-TOF mass spectrometer, calibrated with the Bruker Peptide Calibration Standard II (Billerica, MA). Spectra were analyzed using FlexAnalysis software (Bruker, Billerica, MA).

Stefanski K.M., Russell C.M., Westerfield J.M., Lamichhane R, & Barrera F.N. (2020). PIP2 promotes conformation-specific dimerization of the EphA2 membrane region. The Journal of Biological Chemistry, 296, 100149.

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