Matrigel membrane filter inserts

Manufactured by BD

Sourced in United States, United Kingdom

Matrigel membrane filter inserts are a type of laboratory equipment used for cell culture applications. They consist of a porous membrane coated with a basement membrane extract, providing a specialized microenvironment for cell growth and migration studies.

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Lab products found in correlation

24 well tissue culture plate, by BD (2 mentions)

Spelling variants of this product

Matrigel (17210 citations) Matrigel membrane (28 citations) Transwell-precoated Matrigel membrane filter inserts (10 citations) Matrigel filter (7 citations) Matrigel membrane filter (2 citations) Matrigel-coated Transwell membrane filter inserts (2 citations)

2 protocols using matrigel membrane filter inserts

Cell Migration and Invasion Assays

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Cell migration was evaluated by a wound-healing assay. Cells were plated in six-well dishes and the cell monolayers were scraped using a P-20 micropipette tip. Wound closure was monitored and the percent closure was measured. A cell invasion assay was carried out using modified Boyden Chambers consisting of transwell-precoated Matrigel membrane filter inserts with eight micro pores in 24-well tissue culture plates (BD Biosciences, Bedford, MA, USA). Cells were re-suspended in culture medium without FBS and placed in the upper chamber in triplicate. After 48 h incubation at 37 °C, cells migrating through the membrane were stained. The results were expressed as invaded cells quantified at OD 560 nm.

Mitsui Y., Chang I., Fukuhara S., Hiraki M., Arichi N., Yasumoto H., Hirata H., Yamamura S., Shahryari V., Deng G., Wong D.K., Majid S., Shiina H., Dahiya R, & Tanaka Y. (2015). CYP1B1 promotes tumorigenesis via altered expression of CDC20 and DAPK1 genes in renal cell carcinoma. BMC Cancer, 15, 942.

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Cell Migration and Invasion Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell migration was evaluated by a wound-healing assay. Cells were plated in six-well dishes and after VCAN siRNA treatment for 48 hours, monolayers were scraped using a P-20 micropipette tip. The width of the initial gap (0 hour) and the residual gap 16 or 24 hours after wounding were calculated using a Nikon Eclipse TS100 microscope (Technical Instruments, Burlingame CA). Cell invasion assay was carried out using modified Boyden chambers consisting of transwell-precoated Matrigel membrane filter inserts with 8 µm pores in 24-well tissue culture plates (BD Biosciences, Bedford, MA). Transfected cells were re-suspended in culture medium without FBS and placed in the upper chamber. After 24 hours incubation at 37°C, cells migrating through the membrane were stained. The results were expressed as invaded cells quantified at OD 560 nm using a SPECTRA MAX 190 plate reader.

Mitsui Y., Shiina H., Kato T., Maekawa S., Hashimoto Y., Shiina M., Imai-Sumida M., Kulkarni P., Dasgupta P., Wong R.K., Hiraki M., Arichi N., Fukuhara S., Yamamura S., Majid S., Saini S., Deng G., Dahiya R., Nakajima K, & Tanaka Y. (2017). Versican Promotes Tumor Progression, Metastasis and Predicts Poor Prognosis in Renal Carcinoma. Molecular cancer research : MCR, 15(7), 884-895.

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