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2 protocols using 2 cyclopentenone

1

Prostanoid Compound Acquisition and Characterization

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15-Deoxy-Δ12,14-prostaglandin J2 (15d-PGJ2), CAY10410 (9,10-dihydro-15-deoxy-Δ12,14-prostaglandin J2), prostaglandin D2, prostaglandin E2, prostaglandin F2α, rosiglitazone, and T0070907 were purchased from Cayman Chemical (Ann Arbor, MI). Prostaglandin A1, prostaglandin J2, 4-cyclopentene-1,3-dione, 2-cyclopentenone, cycloheximide, actinomycin D, indomethacin, buthionine sulfoximine, and N-acetylcysteine and uric acid were from Sigma-Aldrich (St Louis, MO). Cyclopentanone and cyclopentene were obtained from Tokyo Chemical Industry (TCI, Portland, OR). Structures of these prostanoids are shown in Table I. Nigericin, lactacystin, and ultrapure lipopolysaccharide (LPS) were purchased from Calbiochem (San Diego, CA).
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2

Characterization of Volatile Compounds

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Authentic standards were obtained from commercial sources: 3-methylbutanal, hexanal, furfural, heptanal, octanal, phenylacetaldehyde, α-pinene, 5-methyl-2-furancarboxaldehyde, nonanal, decanal, ethanol, 1-propanol, 2-butanol, 3-methyl-1-butanol, propylene glycol, 1-pentanol, 2-furanmethanol, 1-hexanol, phenol, 2-methylphenol, 4-methylphenol (p-cresol), 3-methylphenol, 2-methoxyphenol, 2,6-dimethylphenol, 2-methoxy-3-methylphenol, creosol, 4-ethyl-2-methoxyphenol, 2-methoxy-4-vinylphenol, 2,6-dimethoxyphenol, octane, eugenol, trans-isoeugenol, 1-hydroxy-2-propanone, 2-cyclopentenone, cyclooctane, 2-methyl-2-cyclopentenone, 3,4-dimethyl-2-cyclo-pentenone, benzoic acid, 2-hydroxy-3-methyl-2-cyclopentenone, 3-methyl-2-cyclopentenone, acetic acid, 2,3-dimethyl-2-cyclopentenone, 3-ethyl-2-cyclopentenone, butanoic acid, styrene, 3-ethyl-2-hydroxy-2-cyclopentenone, 2,3,4-trimethylpentane, 2-furylmethylketone 2,3,3-trimethylpentane, 2,2,8-trimethyldecane, propanoic acid, 3-methyl-3-heptene, (Z)-2-octene, d-limonene, and ethyl hexanoate were purchased from Sigma-Aldrich (Shanghai, China). 2-Octanol (internal standard) and n-alkane standards (C6–C30) were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). All substances are AR, at least 97% purity. The purification system from Milli-Q (Millipore, Bedford, MA, USA) to obtain purified water.
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