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Cyclin a2 bf683

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Cyclin A2 (BF683) is a primary antibody that recognizes cyclin A2, a protein involved in cell cycle regulation. It can be used for various applications such as Western blotting, immunoprecipitation, and immunohistochemistry.

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Lab products found in correlation

Roswell park memorial institute (rpmi), by American Type Culture Collection (2 mentions) Hek293t cell, by American Type Culture Collection (2 mentions) Cdc2 9112, by Cell Signaling Technology (2 mentions) Ha 7392 antibodies, by Santa Cruz Biotechnology (2 mentions) Flag antibodies a2220, by Merck Group (2 mentions) Gfp 33 2600, by Thermo Fisher Scientific (2 mentions) P21 33 7000, by Thermo Fisher Scientific (2 mentions) P53 13 4100, by Thermo Fisher Scientific (2 mentions) Akt aho1112, by Thermo Fisher Scientific (2 mentions) Phospho akt s473 44 609g, by Thermo Fisher Scientific (2 mentions) Beta actin 8457, by Cell Signaling Technology (2 mentions) Cyclin b1 4138, by Cell Signaling Technology (2 mentions) Anti mouse 7076, by Cell Signaling Technology (2 mentions) Dulbecco modified eagle medium (dmem), by American Type Culture Collection (2 mentions) Hek293t cell line, by American Type Culture Collection (2 mentions) Mage a4, by Cell Signaling Technology (1 mentions) E3012, by Thomas Scientific (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions) Alexa546 conjugated antibodies, by Thermo Fisher Scientific (1 mentions) Chk1 g 4, by Santa Cruz Biotechnology (1 mentions)

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Cyclin A2 (70 citations) BF683 (2 citations)

4 protocols using cyclin a2 bf683

1

Immunoblot Analysis of Cancer Markers

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For immunoblot analysis, cells were lysed in RIPA and whole cell lysates resolved by 10% SDS-PAGE and transferred to PVDF membrane. Immunoblots were probed using the following antibodies where specified: MAGE-A3 IgG1-kappa monoclonal antibody (Proteintech 60054-1-Ig), MAGE-A4 (Cell Signaling (E7O1U) XP Rabbit mAb #82491), p53 (Cell Signaling (1C12) Mouse mAb #2524), Actin IgG1-kappa monoclonal antibody (Thermo Fisher #MA5-11869), GAPDH (Cell Signaling 14C10) and cyclin antibodies (Cell Signaling: Cyclin A2 (BF683), Cyclin B1 (4138), Cyclin D1 (92G2), Cyclin D2 (D52F9), Cyclin D3 (DCS22), and Cyclin E1 (HE12)). Anti-mouse (Cell Signaling 7076s) and anti-rabbit (Cell Signaling 7074s) HRP-conjugated secondary antibodies were used for detection using HRP detection reagent (Thermo Scientific product # 1859698) and autoradiography film (Denville Scientific cat # E3012).
Chen A., Santana A.L., Doudican N., Roudiani N., Laursen K., Therrien J.P., Lee J., Felsen D, & Carucci J.A. (2020). MAGE-A3 is a prognostic biomarker for poor clinical outcome in cutaneous squamous cell carcinoma with perineural invasion via modulation of cell proliferation. PLoS ONE, 15(11), e0241551.
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2

Cell Line Characterization and Antibody Validation

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HEK293T cell line was obtained from the American Type Culture Collection (ATCC; Rockville, MD). RWP1, Mia Paca-2 and Panc02.03 cell lines were kindly provided by Dr. Jason Moffat. HEK293T cells and the pancreatic cancer RWP1, Mia Paca-2 cell lines were maintained in DMEM (ATCC) supplemented with 10% FBS and 1% pen/strep/glutamine, and the appropriate selection antibiotics when required. Panc02.03 cells were maintained in RPMI (ATCC) supplemented with 20% FBS and 1% pen/strep/glutamine, and the appropriate selection antibiotics when required. Antibodies were obtained from the following commercial sources: HA (7392) antibodies (1:2000) were purchased from Santa Cruz. Flag antibodies (A2220) (1:1000) were purchased from Sigma. GFP (33-2600) (1:2000), p21 (33-7000) (1:1000), p53 (13-4100) (1:1000), AKT (AHO1112) (1:1000), phospho-AKT (S473) (44-609G) (1:1000) antibodies were purchased from Invitrogen. Cyclin B1 (4138) (1:1000), cyclin A2 (BF683) (1:2000), cdc2 (9112) (1:1000), beta-actin (8457) (1:1000), anti-mouse (7076) (1:2500) and anti-rabbit (1:2500) HRP-linked (7074) antibodies were purchased from Cell Signaling.
Nim S., Jeon J., Corbi-Verge C., Seo M.H., Ivarsson Y., Moffat J., Tarasova N, & Kim P.M. (2016). Pooled screening for anti-proliferative inhibitors of protein-protein interactions. Nature chemical biology, 12(4), 275-281.
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3

Cell Line Characterization and Antibody Validation

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HEK293T cell line was obtained from the American Type Culture Collection (ATCC; Rockville, MD). RWP1, Mia Paca-2 and Panc02.03 cell lines were kindly provided by Dr. Jason Moffat. HEK293T cells and the pancreatic cancer RWP1, Mia Paca-2 cell lines were maintained in DMEM (ATCC) supplemented with 10% FBS and 1% pen/strep/glutamine, and the appropriate selection antibiotics when required. Panc02.03 cells were maintained in RPMI (ATCC) supplemented with 20% FBS and 1% pen/strep/glutamine, and the appropriate selection antibiotics when required. Antibodies were obtained from the following commercial sources: HA (7392) antibodies (1:2000) were purchased from Santa Cruz. Flag antibodies (A2220) (1:1000) were purchased from Sigma. GFP (33-2600) (1:2000), p21 (33-7000) (1:1000), p53 (13-4100) (1:1000), AKT (AHO1112) (1:1000), phospho-AKT (S473) (44-609G) (1:1000) antibodies were purchased from Invitrogen. Cyclin B1 (4138) (1:1000), cyclin A2 (BF683) (1:2000), cdc2 (9112) (1:1000), beta-actin (8457) (1:1000), anti-mouse (7076) (1:2500) and anti-rabbit (1:2500) HRP-linked (7074) antibodies were purchased from Cell Signaling.
Nim S., Jeon J., Corbi-Verge C., Seo M.H., Ivarsson Y., Moffat J., Tarasova N, & Kim P.M. (2016). Pooled screening for anti-proliferative inhibitors of protein-protein interactions. Nature chemical biology, 12(4), 275-281.
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4

Antibody Characterization for Cell Signaling

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The following commercial antibodies, and the indicated concentrations, were used in this study. C-Myc (#E0115; 1:1000), Chk1 (G-4) (#H2714; 1:1000) and GST (Z-5) (#K0713; 1:1000) were purchased from Santa Cruz Biotechnology. M2 anti Flag Mouse antibody (#SLBT7654; 1:5000), cdc27 (AF3.1) (1:1000) and Actin (#087M4850; 1:10,000) were purchased from Sigma. Cdh1 (#CC43-100UG; 1:500) was purchased from Calbiochem. Cyclin A2 (BF683) (#6; 1:1000), βTRCP1 (D13F10) (1:1000) and Phospho-Chk1Ser345 (133D3) (#15; 1:1000) were obtained from Cell Signaling. HA (#SJ254200; 1:1000) antibody was purchased from Biolegend. Plk1 (3F8) (#06050819; 1:500) was obtained from Enzo Life Sciences. HA antibody (HA.C5 #18181) (1:1000) was purchased from Abcam. Secondary antibodies for western blotting were purchased from LI-COR Biosciences. Anti-phospho-Histone H2AX (clone JBW301) (#2977883, 1:500) was purchased from EMD Millipore Corp. Alexa546-conjugated antibodies (#A11030) for immunofluorescence were purchased from Invitrogen.
Pal D., Torres A.E., Stromberg B.R., Messina A.L., Dickson A.S., De K., Willard B., Venere M, & Summers M.K. (2020). Chk1-mediated phosphorylation of Cdh1 promotes the SCFβTRCP-dependent degradation of Cdh1 during S-phase and efficient cell-cycle progression. Cell Death & Disease, 11(4), 298.
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