All of the fatty acids, i.e., EETs, EpOMEs and HDHAs were obtained from Cayman Chemicals Europe (Tallinn, Estonia). NADPH was from Applichem (Darmstadt, Germany) and tamoxifen was from Sigma Aldrich (Deisenhofen, Germany). Other chemicals were purchased from Merck (Darmstadt, Germany) or Sigma Aldrich. The anti-Cyp2c44 antibody was generated as described [23 (link)], and the antibody directed against aquaporin 4 (AQP4) was from Santa Cruz (TX, USA), the antibody against CD31 was from BD biosciences (Heidelberg, Germany), the phospho histone 3 antibody was from Merck Millipore (Darmstadt, Germany), and the anti-ETS-related gene (ERG) antibody from Abcam (Cambridge, UK).
Hdhas
Manufactured by Cayman Chemical
Sourced in United States
HDHAs are a category of laboratory equipment used for the analysis and quantification of hydroxy-docosahexaenoic acids (HDHAs), which are important lipid molecules. They provide researchers with the tools to accurately measure and investigate the presence and levels of these compounds in various samples.
Automatically generated - may contain errors
2 protocols using hdhas
1
Fatty Acid Quantification and Antibody Validation
2
Lipid Mediator Quantification Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All organic solvents were at least analytical grade and purchased from Sigma-Aldrich (St. Louis, Mo, USA). All isoprostanoids, HDHAs, HETEs, 4-hydroxyhexenal (4-HHE), 4-HNE, and resolvin standards were purchased from Cayman Chemical Co. (Ann Arbor, MI, USA); 8-Hydroxy-2′-deoxyguanosine (8-OHdG) was purchased from Sigma-Aldrich; neuroprotectin (NPD1), dihomo-IsoPs, dihomo-isofurans (dihomo-IsoFs) and NeuroPs were synthesized by the Institut des Biomolécules Max Mousseron (IBMM, Montpellier, France) as previously described [20] [21] [22] [23] [24] . Glutathione (GSH), superoxide dismutase (SOD), catalase (CAT) and bicinchoninic acid (BCA) assay kits were all purchased from Cayman Chemical Co.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!