Agilent 1260 hplc dad

The analysis of egg carotenoids in plasma was conducted using an Agilent 1260 HPLC-DAD and chromatographed using a C30 column (4.6 × 250 mm, 3 μm, YMC Inc., Wilmington, DE, USA) Five hundred μL of plasma was used to extract lutein and zeaxanthin [28 (link),29 (link),30 (link)]. Both carotenoids were quantified at 450 nm using authentic external standard curves.

The analysis of carotenoids in plasma was conducted using an Agilent 1260 HPLC-DAD and chromatographed using a C30 column (4.6 × 250 mm, 3 μm, YMC Inc., Wilmington, DE, USA). A total of 500 μL of plasma was required to extract lutein and zeaxanthin [44 (link),45 (link)]. Both carotenoids were quantified at 450 nm using authentic external standard curves.

An Agilent 1260 HPLC-DAD (Agilent Technologies, CA, USA) with G1311B pump system, G1329A auto-sampler, G1316A temperature control center and G1315D DAD detector were used for chromatographic analysis. Scanning electron microscope (SEM) micrographs of the prepared materials were provided by a Hitachi S-4800 field emission scanning electron microscope (Hitachi, Tokyo, Japan). Fourier transform infrared (FT-IR) spectra (4000–400 cm⁻¹) were achieved on Shimadzu FT-IR-8400S transform infrared spectrometer (Jasco, Kyoto, Japan). X-ray diffraction (XRD) patterns were acquired by a Brucker D8 Venture single crystal X-ray diffractometer (Bruker, Karlsruhe, Germany).