Gene expression data were downloaded from TCGA data repository as level 3 RNA sequence data [4 (link)] created by Illumina RNA Sequencer HiSeq 2000 platforms (Illumina Inc) and annotated with the HG-19 version of the human genome. Normalized and log-transformed gene expression data from these endometrial tumors were available for analysis. Analyses were performed with R statistical packages (R Foundation) for statistical computing and graphics [24 ] and bioconductor packages as open-source software for bioinformatics [25 (link)]. For the front end, we used Biometric Research Branch Array Tools, an integrated package for visualization and statistical analysis that uses Excel (Microsoft Corp) [26 (link)].
Rna sequencer hiseq 2000
Manufactured by Illumina
The Illumina HiSeq 2000 is a high-throughput DNA sequencing system designed for large-scale genomic studies. It utilizes reversible terminator-based sequencing technology to generate billions of sequencing reads per run, making it suitable for a wide range of applications, including whole-genome sequencing, transcriptome analysis, and metagenomics.
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Lab products found in correlation
2 protocols using rna sequencer hiseq 2000
1
Endometrial Tumor Gene Expression Analysis
2
TCGA Endometrial Cancer RNA-Seq Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Normalized and log-transformed gene expression data from the TCGA EC database were downloaded as level 3 RNA-sequenced data. As previously described, data were collected by Illumina RNA sequencer HiSeq 2000 platforms and annotated with the hg19 version of the human genome.
Gene expression analyses were performed with R statistical packages for computing and graphics (The R Foundation) [27 ] and the Bioconductor open-source bioinformatics software package [28 (link)].
Gene expression analyses were performed with R statistical packages for computing and graphics (The R Foundation) [27 ] and the Bioconductor open-source bioinformatics software package [28 (link)].
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