Ab254730

Manufactured by Abcam

Ab254730 is a lab equipment product. It serves a core function, but details about its intended use are not available.

Automatically generated - may contain errors

Lab products found in correlation

Ab172730, by Abcam (1 mentions) Ab227883, by Abcam (1 mentions) Ab230358, by Abcam (1 mentions) Ab8245, by Abcam (1 mentions) Ab32072, by Abcam (1 mentions)

2 protocols using ab254730

Assessing Epigenetic Regulation via ChIP-qRT-PCR

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We added 10% formaldehyde into A2780 cells to form DNA-protein cross-links. After sonicating the DNA-protein cross-links, chromatin fragments were obtained. We divided the chromatin fragments into required parts with equal volume and then generated the immunoprecipitates using anti-TSPAN1 (ab254730, Abcam), anti-TET2 (ab230358, Abcam), anti-DNMT3B (ab227883, Abcam), or normal IgG (ab172730, Abcam). The DNA-protein complexes were precipitated using protein Agarose/Sepharose beads, and qRT-PCR analysis was conducted after cross-link and elution.

Li J., Gao H., Chen B., Li L., Wang Q, & Gao Z. (2022). lncRNA DARS-AS1 Modulates TSPAN1-Mediated ITGA2 Hypomethylation by Interaction with miR-194-5p Thus Promoting Ovarian Cancer Progression. Stem Cells International, 2022, 4041550.

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Protein Expression Analysis via Immunoblotting

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Immunoblotting was carried out as previously described with the following Abs: anti‐p‐ERK (4370; CST), anti‐ERK (4695; CST), anti‐TSPAN1 (ab254730; Abcam), anti‐GAPDH (ab8245; Abcam), and anti‐c‐Myc (ab32072; Abcam).

Han J., Xie C., Liu B., Wang Y., Pang R., Bi W., Sheng R., He G., Kong L., Yu J., Ding Z., Chen L., Jia J., Zhang J, & Nie C. (2023). Tetraspanin 1 regulates papillary thyroid tumor growth and metastasis through c‐Myc‐mediated glycolysis. Cancer Science, 114(12), 4535-4547.

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