Ab108296

At 24 h after the cells were harvested, the protein expression levels were measured by WB analysis. Equal amounts of protein samples were resolved by 10% sodium dodecyl sulfate polyacrylamide gel (SDS-PAGE), and transferred onto a nitrocellulose membrane (Amersham; GE Healthcare, Chicago, IL, USA). The membranes were probed using a polyclonal antibody against CK1α (1:1,000 ab108296; Abcam, Cambridge, UK), CK1ε (1:1,000; sc-373912; Santa Cruz Biotechnology, Inc.), CK1γ (1:1,000; ab64829), CK1δ (1:1,000; ab85320; both Abcam), Bid (1:1,000; 8762), caspase-8 (1:1,000; 4927; both Cell Signaling Technology, Inc., Danvers, MA, USA) at 4°C overnight. Thereafter, membrane was washed with TBST for 3 times, and incubated with secondary antibody at room temperature for 1 h. After wash for 3 times, blots were developed with SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific, Inc.). GAPDH (1:8,000; G5262; Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) was used as an internal control for the comparison of protein load.

Mouse monoclonal anti-β-catenin (SC7963; Santa Cruz Technology), rabbit monoclonal anti-CK1α (ab108296; Abcam), rabbit monoclonal anti-GSK3β (12456; Cell Signaling Technology), rabbit monoclonal anti-β-catenin (S45; 9564; Cell Signaling Technology), rabbit monoclonal anti-β-catenin (S33,37,T41; 2009; Cell Signaling Technology), rabbit monoclonal anti-Axin1 (2074; Cell Signaling Technology), rabbit monoclonal anti-APC (2504; Cell Signaling Technology), mouse monoclonal anti-GFP (scc8334; Santa Cruz Technology), mouse monoclonal anti-MBP (M6295; Sigma-Aldrich), and rabbit monoclonal anti-His-probe (H-15; sc803; Santa Cruz Technology) antibodies, mouse IgG (12-371B; Millipore), and rabbit IgG (12–370; Cell Signaling Technology) were purchased from the indicated suppliers.