Nano z

Manufactured by Malvern Panalytical

Sourced in United Kingdom, United States, Germany, France, Japan, China, Italy

The Nano ZS is a dynamic light scattering (DLS) instrument designed for the measurement of particle size and zeta potential. It is capable of analyzing samples with particle sizes ranging from 0.3 nm to 10 μm. The Nano ZS provides accurate and reliable data on the size distribution and surface charge of a wide range of materials, including nanoparticles, emulsions, and colloids.

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Lab products found in correlation

Zetasizer, by Malvern Panalytical (33 mentions) Jem 2100, by JEOL (12 mentions) Jem 2100f, by JEOL (10 mentions) Ht7700, by Hitachi (9 mentions) Tensor 27, by Bruker (9 mentions) Nicolet is50, by Thermo Fisher Scientific (9 mentions) Mastersizer 2000, by Malvern Panalytical (8 mentions) Jem 1230, by JEOL (8 mentions) Dts1070, by Malvern Panalytical (8 mentions) H 7600, by Hitachi (7 mentions) S 4700, by Hitachi (7 mentions) S 4800, by Hitachi (7 mentions) Orius 1000 ccd camera, by Ametek (6 mentions) Jax 840a, by JEOL (6 mentions) Tecnai f20, by Thermo Fisher Scientific (6 mentions) Escalab 250xi, by Thermo Fisher Scientific (6 mentions) Disposable capillary cell, by Malvern Panalytical (6 mentions) Zetasizer software version 7, by Malvern Panalytical (6 mentions) Nicolet 6700, by Thermo Fisher Scientific (6 mentions) Malvern zetasizer, by Malvern Panalytical (5 mentions)

1 243 protocols using nano z

Physicochemical Characterization of Microemulsions

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The physicochemical characterization of generated microemulsions, including those on droplet size, polydispersity index (PDI), zeta potential, refractive index, electrical conductivity, pH, and viscosity was assessed to determine whether they were suitable for topical application. For each sample, the experiments were run five times at ambient temperature.
The dynamic light scattering method was used to measure the average droplet size and PDI of the microemulsions (Nano ZS, Malvern Instruments, United Kingdom). The data for particle size and PDI were calculated by averaging ten measurements made with disposable cells at 173°. Using disposable flat-folded capillary zeta cells, the zeta potential was measured (Nano ZS, Malvern Zetasizer, UK). The Helmholtz-Smoluchowski equation was used to compute the zeta potential resulting from electrophoretic mobility in an electric field of 40 V/cm. The software took care of the procedure. A viscometer was used to measure the formulations' viscosities (SV-10, AND Vibro Viscometer, Japan). A digital pH meter was used to determine the formulations' pH levels (Mettler Toledo, Switzerland). Using a refractometer, the compositions' refractive index values were assessed (RX-7000 CX, Atago, Japan). To identify the kind of microemulsion, the electrical conductivity of the microemulsions was measured (Nano ZS, Malvern Zetasizer, UK).

Arpa M.D., Çağlar E.Ş., Güreşçi D., Sipahi H, & Üstündağ Okur N. (2024). Novel Microemulsion Containing Benzocaine and Fusidic Acid Simultaneously: Formulation, Characterization, and In Vitro Evaluation for Wound Healing. AAPS PharmSciTech, 25(3).

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Comprehensive Nanoparticle Characterization

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NP size, polydispersity, and superficial
charge are measured by dynamic light scattering (DLS, Zetasizer Nano
ZS, Malvern U.K.). Typically, 1 mL of the diluted sample is put in
12 mm square glass cuvettes for 90° sizing (Optical Cuvette,
Sarstedt) and measured at least in triplicate. ζ potential measurements
are performed at room temperature on a Zetasizer Nano ZS (Malvern,
U.K.), fitted with a high-concentration ζ potential cell. Nanoparticle
morphology is assessed by scanning and transmission electron microscopy,
SEM and TEM, respectively. For SEM observations (Carl Zeiss Ultraplus
Field Emission), 100 μL of purified samples are dropped on a
polycarbonate isopore membrane filter (cutoff 0.05 μm) under
vacuum ultrafiltration and let dry overnight. Before the observation,
5–7 nm of Au is deposited on the sample. For cryo-TEM observation
of EVs (Cryo-TEM TECNAI by FEI), 5 μL of the purified sample
is dropped on a Formvar/Carbon 200 mesh grid (Agar scientific) and
observed at an 80 kV accelerating voltage. The concentration of cHANPs
and EVs is quantified by nanoparticle tracking analysis NTA (Nanosight
NS300, Malvern Instruments Ltd., U.K.). Both samples are observed
at a dilution of 1:100, at 25 °C for 300 s with a manual shutter
and gain adjustment. Results are analyzed with NP Tracking Analysis
software.

Costagliola di Polidoro A., Baghbantarghdari Z., De Gregorio V., Silvestri S., Netti P.A, & Torino E. (2023). Insulin Activation Mediated by Uptake Mechanisms: A Comparison of the Behavior between Polymer Nanoparticles and Extracellular Vesicles in 3D Liver Tissues. Biomacromolecules, 24(5), 2203-2212.

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Formulation and Characterization of AuNR-EPI Complex

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In order to form AuNR.EPI complex, AuNRs (100 µg) were added to EPI solutions (0.5 µg/µL in 0.01 M PBS at pH =7.4) at different mass ratios (MRs), followed by 12-hour incubation with 400 rpm shaking at 45°C. Following incubation, the uncomplexed EPI was removed by centrifugation at 6,000 rpm for 20 minutes and measured using UV-visible spectrophotometer at 480 nm, in order to determine the efficiency of AuNRs to form complex with EPI. Particle size and zeta potential of AuNR.EPI complex were assessed using the Malvern Nano-ZS as described in the section “Dynamic light scattering”.
In addition, the AuNR.EPI complex (MR =5) was incubated within the saline at 4°C and 37°C for 6, 24, and 48 hours. The particle size and zeta potential of AuNR. EPI complex were assessed using the Malvern Nano-ZS as described in the section “Dynamic light scattering”.
To evaluate the photothermal conversion efficiency, 200 µL of PBS, EPI (0.2 µg/µL), blank AuNRs (1 µg/µL), and AuNR.EPI formulation (AuNR =1 µg/µL; MR =5) were irradiated by 808 nm NIR laser with an intensity of 2.5 W/cm⁻² (BWT; Diode Laser System, Beijing, China). The temperature change was monitored by the infrared thermal analysis system (BM_IR; Beetech, Beijing, China) for 4.5 minutes at an interval of 0.5 min per reading.

Wang L., Pei J., Cong Z., Zou Y., Sun T., Davitt F., Garcia-Gil A., Holmes J.D., O’Driscoll C.M., Rahme K, & Guo J. (2019). Development of anisamide-targeted PEGylated gold nanorods to deliver epirubicin for chemo-photothermal therapy in tumor-bearing mice. International Journal of Nanomedicine, 14, 1817-1833.

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Characterizing Microparticle Size and Charge

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The particle size and zeta potential of the unloaded blank and the C8-HSL-loaded MPs were measured using a Malvern Nano ZS, in triplicate. This instrument is based on the principle that utilizes a laser light to illuminate the particle and is able to analyze the fluctuation in the intensity of scattered light. The principle of dynamic light scattering is that particles and molecules which are in constant random thermal motion, called Brownian motion, can diffuse at a speed related to their size. The charge acquired by a particle in a medium is its zeta potential and arises from the surface charge and is also dependent on the concentration and types of ions in the solution. Next, 2 mg microparticles were suspended in 1 mL of deionized water [1 (link)]. The size and charge of the particles were measured using a Malvern Nano ZS. The particle size of C8-HSL MP was 4.43 ± 0.29 μm. The particle sizes of all adjuvant MP ranged from 2 to 4 μm. The particle sizes of all antigens’ MPs ranged from 2 to 5 μm.

Shah S.M., Joshi D., Chbib C., Roni M.A, & Uddin M.N. (2023). The Autoinducer N-Octanoyl-L-Homoserine Lactone (C8-HSL) as a Potential Adjuvant in Vaccine Formulations. Pharmaceuticals, 16(5), 713.

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Characterization of PLGA Nanoparticles

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NPs were sized using both imaging techniques (SEM) and dynamic light scattering (DLS). For SEM imaging, dry PLGA BPNPs (without trehalose) were placed on carbon tape and sputter coated with gold for 30 sec under 40 mA current (Sputter Coated 180aute, Cressington). Images were acquired using a XL-30 ESEM-FEG (FEI Company, Hillsboro, OR, USA) under 10 kV acceleration voltage. Average nanoparticle size was quantified using the ImageJ software (National Institutes of Health, Bethesda, MD, USA), where sample populations of at least 1,000 NPs were included for statistical analysis.
Hydrodynamic size analysis was completed using DLS. BPNPs were resuspended at 0.05 mg/mL in DI water and sized using a Malvern Nano-ZS. Results are reported as the Z-average diameter, corresponding to the hydrodynamic diameter of the particles. In all cases, the polydispersity index was less than 0.2. To measure surface charge (zeta potential), BPNPs were diluted in DI water at a concentration of 0.5 mg/mL; 750 μL of solution was loaded into a disposable capillary cell (Malvern, UK) and the charge was measured using a Malvern Nano-ZS.

Saucier-Sawyer J.K., Seo Y.E., Gaudin A., Quijano E., Song E., Sawyer A.J., Deng Y., Huttner A, & Saltzman W.M. (2016). Distribution of Polymer Nanoparticles by Convection-Enhanced Delivery to Brain Tumors. Journal of controlled release : official journal of the Controlled Release Society, 232, 103-112.

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DLS and Zeta Potential Analysis of SiNPs

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DLS measurements of SiNP solution (3 μg/mL)
as well as different fibrillar samples (5 μM) were carried out
on a Malvern Nano ZS instrument fitted with a 4 mW He–Ne laser
beam (λ = 632.8 nm, scattering angle of 173°) and a thermostatic
sample chamber at 25 °C. For zeta potential measurements of the
samples (7 μM), a Malvern Nano ZS instrument equipped with the
same accessories as mentioned for DLS studies was used.

Konar M., Mathew A, & Dasgupta S. (2019). Effect of Silica Nanoparticles on the Amyloid Fibrillation of Lysozyme. ACS Omega, 4(1), 1015-1026.

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Physicochemical Characterization of Morin Nanoparticles

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The average size and the size distribution of the prepared morin NPs were measured at 25°C±1°C by dynamic light scattering, with NanoZS (Malvern Instruments, Malvern, UK). All the NP samples were appropriately diluted with MilliQ water to get optimum (60–200) kilo counts per second (Kcps) for measurement at a temperature of 25°C±1°C. Zeta potential of the NPs was determined by combination of laser Doppler velocimetry and phase analysis light scattering method using a NanoZS (Malvern Instruments). The size, surface morphology, and shape of the freeze-dried NPs were assessed using transmission electron microscopy (TEM) (Morgagni TEM, FEI, Eindhoven, Netherlands) and atomic force microscopy (AFM) (Bruker, Innova AFM, Karlsruhe, Germany).

Shetty P.K., Venuvanka V., Jagani H.V., Chethan G.H., Ligade V.S., Musmade P.B., Nayak U.Y., Reddy M.S., Kalthur G., Udupa N., Rao C.M, & Mutalik S. (2015). Development and evaluation of sunscreen creams containing morin-encapsulated nanoparticles for enhanced UV radiation protection and antioxidant activity. International Journal of Nanomedicine, 10, 6477-6491.

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Nanoparticle Size and Charge Analysis by PCS and Zeta Potential

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The mean particle size (Z-Average) and polydispersity index (PDI) of all samples were determined by photon correlation spectroscopy (PCS) analysis, using a Zetasizer Nano ZS (Malvern, Milan, Italy; laser 4 mW He–Ne, 633 nm, laser attenuator automatic, transmission 100–0.0003%, detector avalanche photodiode, Q.E. > 50% at 633 nm, T = 25 °C).
All samples were diluted before being analyzed: 10 µL of purified NPs suspension were diluted with MilliQ water to 1 mL to arrive at a final concentration of ~0.1 mg/mL. At least three individual NP formulations were prepared and analyzed to yield a mean for each data set.
The zeta potential (ζ-pot) was measured using a Zetasizer Nano ZS (Malvern, Milan, Italy) with a combination of laser doppler velocimetry and a patented phase analysis light scattering method (M3-PALS). The same samples subjected to PCS (0.1 mg/mL) were analyzed using DTS1070 ζ-pot cuvettes and expressed as the mean of at least three individual NP preparations.

Duskey J.T., Ottonelli I., Rinaldi A., Parmeggiani I., Zambelli B., Wang L.Z., Prud’homme R.K., Vandelli M.A., Tosi G, & Ruozi B. (2021). Tween® Preserves Enzyme Activity and Stability in PLGA Nanoparticles. Nanomaterials, 11(11), 2946.

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Zeta Potential Characterization of Nanoparticles

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Zetasizer was also used to determine the surface charge of the prepared samples at ambient temperature (Nano ZS, Malvern Instruments, Malvern, UK). Zetasizer was also used to determine the surface charge of the prepared samples at ambient temperature (Nano ZS, Malvern Instruments, Malvern, UK). The zeta values were obtained by the electrophoretic mobility of nanoparticles moving into an applied electric field.

Fahmy H.M., Ahmed M.M., Mohamed A.S., Shams-Eldin E., Abd El-Daim T.M., El-Feky A.S., Mustafa A.B., Abd Alrahman M.W., Mohammed F.F, & Fathy M.M. (2022). Novel lipid-coated mesoporous silica nanoparticles loaded with thymoquinone formulation to increase its bioavailability in the brain and organs of Wistar rats. BMC Pharmacology & Toxicology, 23, 71.

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Niosome Size and Zeta Potential

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The size distribution of niosomes was measured by dynamic light scattering (DLS) using a Zetasizer Nano ZS (Malvern) instrument and a nanoparticle tracking system, Nanosight (NS300, Malvern). The zeta (ζ) potential was measured on a Zetasizer Nano ZS instrument using folded capillary cells from Malvern.

Pereira M.C., Pianella M., Wei D., Moshnikova A., Marianecci C., Carafa M., Andreev O.A, & Reshetnyak Y.K. (2017). pH-sensitive pHLIP® Coated Niosomes. Molecular membrane biology, 33(3-5), 51-63.

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