Sh mettl3

Manufactured by Sangon

Sourced in China

Sh-METTL3 is a short hairpin RNA (shRNA) targeting the METTL3 gene, which is responsible for the N6-methyladenosine (m6A) modification of RNA. The Sh-METTL3 product is designed for the knockdown of METTL3 expression in cell-based studies.

Automatically generated - may contain errors

Lab products found in correlation

Lipofectamine 3000 reagent, by Thermo Fisher Scientific (1 mentions) Lipofectamine 3000 transfection reagent, by Takara Bio (1 mentions)

3 protocols using sh mettl3

METTL3 Overexpression Protocol

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Cells in the log growth phase were transfected with overexpressed methyltransferase-like 3 (METTL3), negative control vector (sh-METTL3), and negative control shRNA (Sangon, China) using Lipofectamine 3000 reagent (Invitrogen, CA, USA) according to a previous study [20 (link)].

Wang B., Liu Y., Jiang R., Liu Z., Gao H., Chen F, & Mei J. (2022). Emodin relieves the inflammation and pyroptosis of lipopolysaccharide-treated 1321N1 cells by regulating methyltransferase-like 3 -mediated NLR family pyrin domain containing 3 expression. Bioengineered, 13(3), 6740-6749.

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Plasmid Transfection of ADSCs

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For cell transfection, sh-METTL3, sh-IGF2BP2, pcDNA-VEGF-C and pcDNA-METTL3 plasmids were synthesized by Sangon Biotech (Shanghai, China), and the negative controls were sh-NC and pcDNA-NC. According to the manufacturer's instructions, transfection was carried out using Lipofectamine™ 3000 Transfection Reagent (Takara, Kusatsu, Japan). Forty-eight hours after transfection, ADSCs were used in the follow-up experiment.

Zhou J., Wei T, & He Z. (2021). ADSCs enhance VEGFR3-mediated lymphangiogenesis via METTL3-mediated VEGF-C m6A modification to improve wound healing of diabetic foot ulcers. Molecular Medicine, 27, 146.

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Cell Transfection with shRNA Targeting METTL3 and GPX4

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Accordingly to a previous study [19 (link)], cell transfection was performed when the cells reached approximately 60% confluence. The short hairpin RNA METTL3 (sh-METTL3) and GPX4 (sh-GPX4) were obtained from Sangon (Shanghai, China). Next, the cells were incubated with 5 μl Lipofectamine® 3000 reagent and 5 μl transfection plasmids. After transfection, the cells were collected for subsequent experiments.

Zhang L., Wang X., Che W., Yi Y., Zhou S, & Feng Y. (2022). Methyltransferase-like 3 silenced inhibited the ferroptosis development via regulating the glutathione peroxidase 4 levels in the intracerebral hemorrhage progression. Bioengineered, 13(6), 14215-14226.

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