All chemicals were of analytical grade and used as received without further purification. Cotton cellulose from filter paper (Q1, Whatman, Maidstone, UK) was supplied by Fisher Scientific (Pittsburgh, PA, USA). Sulfuric acid (95%–98%, reagent grade) was purchased from Scharlau (Barcelona, Spain). Ethanol and sodium hydroxide were provided by Sigma-Aldrich (St Louis, MO, USA). Zinc acetate dehydrate (99%) and silver nitrate (99.98%), used as precursors, were provided by Merck (Darmstadt, Germany). Cs with an 85% degree of deacetylation and an average molecular weight of “345,500” g/mol was obtained from the Malaysian Nuclear Agency (Selangor, Malaysia). PVA with 99% hydrolysis and an average molecular weight of 89.000 g/mol was purchased from Sigma-Aldrich.
Zinc acetate dehydrate
Manufactured by Merck Group
Sourced in United States, Germany, Latvia
Zinc acetate dehydrate is a chemical compound with the formula Zn(CH3COO)2·2H2O. It is a white crystalline solid that is soluble in water and organic solvents. Zinc acetate dehydrate is commonly used as a source of zinc in various applications, including pharmaceutical and chemical industries.
Automatically generated - may contain errors
Lab products found in correlation
Sodium hydroxide (naoh), by Merck Group (14 mentions)
Potassium hexacyanoferrate 2 trihydrate, by Merck Group (11 mentions)
Formic acid, by ITW Reagents (9 mentions)
13c3 labelled acrylamide, by Cambridge Isotopes (9 mentions)
Methanol, by Merck Group (8 mentions)
Ethanolamine, by Merck Group (7 mentions)
Methanol, by ITW Reagents (7 mentions)
Zinc nitrate hexahydrate, by Merck Group (7 mentions)
Silver nitrate, by Merck Group (5 mentions)
Ethanol, by Merck Group (5 mentions)
Milli q integral 5 water purification system, by Merck Group (5 mentions)
Hexamethylenetetramine, by Merck Group (5 mentions)
Hydrochloric acid (hcl), by Merck Group (5 mentions)
Ammonium hydroxide, by Merck Group (4 mentions)
2 methoxyethanol, by Merck Group (4 mentions)
Reversed phase oasis hlb cartridges, by Waters Corporation (4 mentions)
D glucose, by ITW Reagents (4 mentions)
Integral 5 water purification system, by Merck Group (3 mentions)
Hexane, by ITW Reagents (3 mentions)
Acrylamide standard, by Merck Group (3 mentions)
66 protocols using zinc acetate dehydrate
1
Synthesis of Cellulose-Based Nanocomposites
2
Synthesis and Antimicrobial Testing of 4A z
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Here, 4A z (Si/Al ≅ 2) was synthesized according to the formula below. Zinc acetate dehydrate, orthotitanate and ethanol were purchased from Merck. All the applied reagents were of analytical grade. It should be noted that in order to test antimicrobial activity, bacterial strains were obtained from Biological and Genetic Resource Center, Tehran, Iran. Nutrient broth and Nutrient agar were all purchased from Micromedia, Canada.
3
Synthesis of Zinc Acetate Nanoparticles
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The zinc acetate dehydrate was obtained from Merck Millipore (Darmstadt, Germany). The Ammonium hydroxide, XG and LB broths (Lysogeny broth) were purchased from Sigma-Aldrich (St. Louis, Missouri, USA). All the solutions used for the fabrication of nanoparticles were prepared using ultrapure water through a Millipore water purification system (Milli-Q, Milford, MA, USA). Ampicillin discs (10 µg) and blank paper discs (6 mm diameter) were obtained from Fort Richard Laboratories (Auckland, New Zealand).
4
Microwave-Assisted Synthesis of Zinc Hydroxide
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First, 0.02 moles of zinc acetate dehydrate (Merck, Germany) was dissolved in 50 mL of deionized water. Then, 0.40 moles of solid sodium hydroxide (Merck, Germany) was added slowly into the solution under magnetic stirring at room temperature, forming a transparent Zn (OH)42- solution. Thereafter, 2 mL of ionic liquid, 1-butyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide, [bmim][NTf2], was added to 3 mL of the above solution. The suspension was put into a domestic microwave oven (2.45 GHz, 850 W) in air. Of the output power of the microwave, 30% was used to irradiate the suspension for five minutes in a cycling mode (on for 10 seconds, off for five seconds). The white precipitate was collected by centrifugation (15000 rpm for 15 minutes), washed with deionized water and ethanol, and dried in vacuum oven at 40°C for 10 hours (11 ). Phase and purity of the products were analyzed using D8 Advanced diffractometer with Cu Kα radiation (λ = 0.15406 nm). The transmission electron microscopy was recorded by the LEO system (model 912 AB), operating at 120 kV for the samples.
5
Synthesis of ZnO Nanoparticles
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All chemicals used were analytical grade. Ultra-pure water was used for the preparation of all reagents solutions. The materials used for the synthesis of the ZnO NPs were purchased: zinc acetate dehydrate (Zn(O2CCH3)2(H2O)2) as the zinc precursor, terephthalic acid (C₆H₄(CO₂H)₂) as ligand, tri-ethylamine (N(CH₂CH₃)₃) as capping agent, dimethylformamide (C3H7NO) as solvent from Merck (Germany).
6
Analytical Determination of Mycotoxins
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All chemicals
and reagents
were of analytical grade and utilized without additional purification.
Aflatoxin B1 (AFB1), aflatoxin G1 (AFG1), aflatoxin G2 (AFG2), aflatoxin
B2 (AFB2), zearalenone (ZEN), deoxynivalenol
(DON), ochratoxin A (OTA), methanol, Cur, zinc acetate dehydrate, N,N-dimethylformamide (DMF), boric acid,
and sodium hydroxide were purchased from Merck Sigma-Aldrich Pvt.
Ltd., USA. The AFs and other mycotoxins mother stocks are kept in
an amber flask in a closed and refrigerated chamber at −20
°C. Bentonite (aluminum silicate hydrate montmorillonite) was
purchased from SRL Pvt. Ltd., India. Ultrapure water (18.2 MΩ·cm)
utilized in the experiment was obtained from a Milli-Q purification
system (Millipore), and NaOH, boric acid, and ZnOBt dispersion were
made with water. The respective standard solution of mycotoxins and
individual aflatoxins (in the ratio of 1.0:0.1:0.3:0.03) were mixed
in methanol at a ratio of 50 μg mL–1. The
required working solution was prepared with a methanol–water
solvent in a ratio of 3/7 (V/V).
and reagents
were of analytical grade and utilized without additional purification.
Aflatoxin B1 (AFB1), aflatoxin G1 (AFG1), aflatoxin G2 (AFG2), aflatoxin
B2 (AFB2), zearalenone (ZEN), deoxynivalenol
(DON), ochratoxin A (OTA), methanol, Cur, zinc acetate dehydrate, N,N-dimethylformamide (DMF), boric acid,
and sodium hydroxide were purchased from Merck Sigma-Aldrich Pvt.
Ltd., USA. The AFs and other mycotoxins mother stocks are kept in
an amber flask in a closed and refrigerated chamber at −20
°C. Bentonite (aluminum silicate hydrate montmorillonite) was
purchased from SRL Pvt. Ltd., India. Ultrapure water (18.2 MΩ·cm)
utilized in the experiment was obtained from a Milli-Q purification
system (Millipore), and NaOH, boric acid, and ZnOBt dispersion were
made with water. The respective standard solution of mycotoxins and
individual aflatoxins (in the ratio of 1.0:0.1:0.3:0.03) were mixed
in methanol at a ratio of 50 μg mL–1. The
required working solution was prepared with a methanol–water
solvent in a ratio of 3/7 (V/V).
7
Synthesis of Metal-Organic Frameworks
8
Nanoparticle-based Doxorubicin Delivery for Breast Cancer Treatment
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Indocyanine green and sodium hydroxide were obtained from Beijing Tong Guang Fine Chemicals Company (Beijing, China). Zinc acetate dehydrate was purchased from Sigma-Aldrich (St. Louis, MO, USA). Doxorubicin was obtained from Ouhe Technology Co., Ltd. (Beijing, China). Pluronic was purchased from Beijing Solable Technology Co., Ltd. (Beijing, China). The ultrafiltration tubes were purchased from Merck Millipore (Darmstadt, Germany). The RPMI 1640 cell culture medium was obtained from Macgene Biotech Co. Ltd. (Beijing, China). Fetal bovine serum was purchased from GIBCO (Carlsbad, CA, USA).
The mammary breast cancer cell line 4T1 was purchased from the Cell Resource Center, Peking Union Medial College (Beijing, China). The cells were cultured according to the recommended conditions from ATCC.
The female BALB/C mice (6 weeks) were provided by the Experimental Animal Center of the Peking University Health Science Center. The animal experimental procedures were approved by the Ethics Committee of Peking University. The project identification code was LA2021366. The date of approval was 3 June 2021.
The mammary breast cancer cell line 4T1 was purchased from the Cell Resource Center, Peking Union Medial College (Beijing, China). The cells were cultured according to the recommended conditions from ATCC.
The female BALB/C mice (6 weeks) were provided by the Experimental Animal Center of the Peking University Health Science Center. The animal experimental procedures were approved by the Ethics Committee of Peking University. The project identification code was LA2021366. The date of approval was 3 June 2021.
9
Colorimetric Iron Quantification
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Silver nitrate (AgNO3) ≥ 99%, Iron (II) sulfate heptahydrate ≥ 99% (FeSO4·7H2O), Iron (III) chloride 97% (FeCl3), Zinc acetate dehydrate 99.9% Zn(CH3CO2)2·2H2O, Standard Hemoglobin (Hb) powder (≥ 99.9%), Sodium nitrite (NaNO2) ≥ 99%, absolute ethanol ≥ 99.8% (CH3CH2OH), Sodium hydroxide 99.99% (NaOH), Ammonium hydroxide (NH4OH) 30% solution, Monopotassium dihydrate phosphate 98% (KH2PO4), Dipotassium monohydrate phosphate 98% (K2HPO4), Trisodium citrate 99% (C6H5O7Na3), and Citric acid 99.99% (C6H8O7) were purchased from Sigma Aldrich USA.
10
Synthesis and Characterization of Graphene Oxide
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The following chemicals, graphite powder (Sigma–Aldrich, 10 mesh), sulfuric acid (Sigma–Aldrich, ACS reagent, 95.0–98.0%), hydrochloric acid (Sigma–Aldrich, ACS reagent, 37%), potassium permanganate (Fischer Scientific, ≥99%), hydrogen peroxide (Sigma–Aldrich, 30 wt% in H2O), sodium hydroxide (Sigma–Aldrich, ACS reagent, ≥97.0%), zinc acetate dehydrate (Sigma–Aldrich, ACS reagent, ≥98%) and phosphoric acid (Sigma–Aldrich, ACS reagent, ≥85 wt% in H2O). Scavengers p-benzoquinone (BQ), t-Butanol (TBA), ethylenediaminetetraacetic acid disodium salt (EDTA-2Na), and AgNO3 (all Sigma–Aldrich) were used as received. Zinc hydroxyacetate was prepared by procedure presented in our previous article28 (link).
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