Chemidoc system

Manufactured by Bio-Rad

Sourced in United States, Germany, United Kingdom, Italy, China, Canada, Belgium, Singapore

The ChemiDoc system is a compact imaging system designed for the detection and analysis of chemiluminescent, fluorescent, and colorimetric signals in life science applications. It provides a versatile platform for various imaging techniques, including Western blotting, gel documentation, and multiplex protein detection.

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Lab products found in correlation

Image lab software, by Bio-Rad (87 mentions) Pierce bca protein assay kit, by Thermo Fisher Scientific (58 mentions) Pvdf membrane, by Merck Group (42 mentions) Pvdf membrane, by Bio-Rad (26 mentions) Nitrocellulose membrane, by Bio-Rad (25 mentions) Quantity one software, by Bio-Rad (24 mentions) Trans blot turbo transfer system, by Bio-Rad (21 mentions) Clarity western ecl substrate, by Bio-Rad (20 mentions) Bca protein assay kit, by Thermo Fisher Scientific (18 mentions) Protease inhibitor cocktail, by Merck Group (18 mentions) Supersignal west femto maximum sensitivity substrate, by Thermo Fisher Scientific (17 mentions) Gapdh, by Cell Signaling Technology (13 mentions) Image lab, by Bio-Rad (12 mentions) Ripa buffer, by Thermo Fisher Scientific (12 mentions) Ripa buffer, by Merck Group (11 mentions) β actin, by Merck Group (11 mentions) Pierce ecl western blotting substrate, by Thermo Fisher Scientific (11 mentions) Protease inhibitor cocktail, by Roche (11 mentions) Laemmli sample buffer, by Bio-Rad (11 mentions) Trans blot turbo system, by Bio-Rad (9 mentions)

837 protocols using chemidoc system

Spo0A Protein Expression Analysis in Sporulating Bacteria

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The indicated strains were grown on 70:30 sporulation agar supplemented with 2 μg/ml thiamphenicol and 0.5 μg/ml nisin and harvested at H₁₂. Total protein from the cell lysates was quantitated using the Pierce Micro bicinchoninic acid (BCA) protein assay kit (Thermo Scientific), 2.5 μg of total protein was separated by electrophoresis on a precast 4 to 20% TGX stain-free gradient gel (Bio-Rad), and total protein was imaged using a ChemiDoc system (Bio-Rad). Protein was transferred to a 0.45-μm nitrocellulose membrane, and Western blot analysis was performed with mouse anti-Spo0A (71 (link)) as the primary antibody and goat anti-mouse conjugated with Alexa Fluor 488 (Invitrogen) as the secondary antibody. Imaging and densitometry were performed with a ChemiDoc system and Image Lab software (Bio-Rad), respectively, for three independent experiments.

Edwards A.N., Willams C.L., Pareek N., McBride S.M, & Tamayo R. (2021). c-di-GMP Inhibits Early Sporulation in Clostridioides difficile. mSphere, 6(6), e00919-21.

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Hydrolysis of Cer by LDL(−)

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To evaluate the ability of LDL(−) to hydrolyze external Cer, LDL(+) and LDL(−) (75 μg apoB) were incubated with CER labeled in the fatty acid moiety with 12-(N-(7-Nitrobenz-2-Oxa-1,3-Diazol-4-yl)amino)hexanoyl) (NBD-CER; Avanti Polar Lipids). The incubation was performed with the fluorescent substrate at 4 μM for 4, 20, and 48 h at 37 °C in the presence or absence of 10 μM MAPP. Total lipids were then extracted and applied to a TLC plate to detect the fluorescent products in a ChemiDoc system (BioRad), as described in [12 (link)]. C12 Cer-labeled (NBD-Cer) and arachidonic acid fatty acid-labeled (NBD-NEFA; both 2 μg) were used as the controls for TLC, which was performed with the same three phases described in 4.3. The fluorescence bands were detected using the ChemiDoc system (Biorad).

Puig N., Rives J., Estruch M., Aguilera-Simon A., Rotllan N., Camacho M., Colomé N., Canals F., Sánchez-Quesada J.L, & Benitez S. (2022). Presence of Ceramidase Activity in Electronegative LDL. International Journal of Molecular Sciences, 24(1), 165.

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Western Blot Analysis of Protein Expression

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Antibodies used in this study are listed in Table S3. For binding assays, samples were run on 4–15% gradient Mini-Protean gels (BioRad) and transferred to PVDF membranes using a Transblot Turbo system (BioRad). The membranes were incubated in blocking buffer (5% skim milk in TBS-Tween 0.05%) for one hour, and antibodies were incubated in either the same buffer or in TBS-T + 5% BSA. Membranes were developed using Western Bright Sirius substrate (Advansta) before imaging on a ChemiDoc system (BioRad).
For blots of worm lysates, 4–12% NuPAGE Bis-Tris gels (Invitrogen) were loaded with lysate equal to 5–10 adult worms. Proteins were transferred overnight to nitrocellulose membranes, after which membranes were blocked and antibodies incubated in TBS-T with 5% milk. Membranes were developed using Western Bright Sirius substrate (Advansta) before imaging on ChemiDoc system (BioRad).

Houston J., Vissotsky C., Deep A., Hakozaki H., Crews E., Oegema K., Corbett K.D., Lara-Gonzalez P., Kim T, & Desai A. (2024). Phospho-KNL-1 recognition by a TPR domain targets the BUB-1–BUB-3 complex to C. elegans kinetochores. bioRxiv.

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Western Blot Analysis of Phosphorylated eIF2α

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Snap-frozen tissues were homogenized in ice-cold homogenization buffer (0.25 M sucrose, 1 mM EDTA, 1 mM DTT, pH 7) containing HALT protease and phosphatase inhibitors (Thermo Fisher Scientific) using a hand-held disperser (Ultra-Turrax, IKA, Germany). Samples were centrifuged for 15 min at 1,000 g at 4°C. The infranatant was collected, and protein concentration was assayed using the Bio-Rad Protein Assay Dye Reagent (Bio-Rad Laboratories GmbH, Germany). Proteins were separated using SDS-PAGE, and proteins were transferred to a polyvinylidene difluoride membrane (Bio-Rad Laboratories GmbH). Specific proteins were detected using the anti-rabbit peIF2 alpha (Ser51) (1:1,000 dilution, catalog no.: 3398, Cell Signaling Technology, MA) and eIF2 alpha (1:1,000 dilution, catalog no.: 5324, Cell Signaling Technology). Alpha tubulin (1:1,000 dilution, catalog no.: ab52866, Abcam, UK) was used as a loading control. As secondary antibody, HRP-conjugated goat anti-rabbit antibody (Cell Signaling Technology) was used. Proteins were visualized using Clarity™ substrate and the ChemiDoc™system (Bio-Rad Laboratories GmbH, Germany). Signal density was determined with ImageJ (29 ) or directly the ChemiDoc™system (Bio-Rad Laboratories GmbH).

Kotzbeck P., Taschler U., Haudum C., Foessl I., Schoiswohl G., Boulgaropoulos B., Bounab K., Einsiedler J., Pajed L., Tilp A., Schwarz A., Eichmann T.O., Obermayer-Pietsch B., Giordano A., Cinti S., Zechner R, & Pieber T.R. (2022). Rosiglitazone Reverses Inflammation in Epididymal White Adipose Tissue in Hormone-Sensitive Lipase-Knockout Mice. Journal of Lipid Research, 64(1), 100305.

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Click-Reaction Sensitivity Validation

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2D-gels with 10-undecyn-1-ol treated cell lysates, which served as a negative control to verify the sensitivity of the click-reaction, were subjected to Coomassie-blue staining for 2 h to reveal the overall protein pattern. Imaging was carried out on a ChemiDoc™ System.

Prasch J., Bernhart E., Reicher H., Kollroser M., Rechberger G.N., Koyani C.N., Trummer C., Rech L., Rainer P.P., Hammer A., Malle E, & Sattler W. (2020). Myeloperoxidase-Derived 2-Chlorohexadecanal Is Generated in Mouse Heart during Endotoxemia and Induces Modification of Distinct Cardiomyocyte Protein Subsets In Vitro. International Journal of Molecular Sciences, 21(23), 9235.

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Transient Expression of CRKs in Arabidopsis and Nicotiana

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For Arabidopsis protoplast transient expression, protoplasts from WT or stt3a mutant were transfected with HA-tagged CRKs in the pHBTvector and incubated for 12 h. Proteins were isolated with 2×SDS loading buffer and subjected to immunoblot analysis with anti-HA antibody.
For N. benthamiana transient expression, Agrobacterium tumefaciens strain GV3101 containing pCB302 vector was cultured overnight in LB medium at 28 °C. Bacteria were harvested by centrifugation and resuspended with buffer (10 mM MES, pH 5.7, 10 mM MgCl₂, 200 μM acetosyringone) at A₆₀₀ = 0.75. Leaves of 4-week-old soil-grown N. benthamiana were hand-infiltrated using a needleless syringe with Agrobacterium cultures. Leaf samples were collected 36 h after infiltration for protein isolation and immunoblot analysis. The cell death phenotype was observed and leaf pictures were taken 4 days after infiltration under UV light with a ChemiDoc system.

de Oliveira M.V., Xu G., Li B., de Souza Vespoli L., Meng X., Chen X., Yu X., de Souza S.A., Intorne A.C., de A. Manhães A.M., Musinsky A.L., Koiwa H., de Souza Filho G.A., Shan L, & He P. (2016). Specific control of Arabidopsis BAK1/SERK4-regulated cell death by protein glycosylation. Nature plants, 2, 15218.

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Western Blot Protein Analysis

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Samples were thawed on ice and boiled for 5 minutes at 90°C. After use of crescendo, 20 µl of protein was loaded into a 10 well 4–20% gel. The gel was run at 100 mV for 1 hour, and transferred to a nitrocellulose membrane overnight at 4°C. Following a rinse with TBS, nonspecific sites were blocked with 5% milk in TBST for 1 hour at RT. Primary antibodies were applied at 1:2500 concentration in 5% milk in TBST overnight at 4°C. Following three 5 minute washes with TBST, secondary antibodies were applied at a 1:10000 concentration in 5% milk in TBST overnight at 4°C. After 3 washes with TBST, the membrane was incubated with Crescendo for 1 minute. Membranes were digitally imaged on a ChemiDoc system.

Delic V., Chandra S., Abdelmotilib H., Maltbie T., Wang S., Kem D., Scott H.J., Underwood R.N., Liu Z., Volpicelli-Daley L.A, & West A.B. (2018). Sensitivity and Specificity of Phospho-Ser129 α-Synuclein Monoclonal Antibodies. The Journal of comparative neurology, 526(12), 1978-1990.

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Agarose Gel Electrophoresis of BRD4-siRNA Lipoplexes

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siRNA easily undergoes ribonucleolytic degradation, while the lipoplex form enhances the stability, agarose gel electrophoresis is used to study the formation of BRD4-siRNA-LP (Patil et al., 2011 (link)). In this study, to test the stability of BRD4 siRNA in lipoplexes, they were prepared and loaded on to the gel by mixing in 1X loading dye. Plain BRD4 siRNA was employed for comparison. 3% agarose gel was prepared by adding ethidium bromide and electrophoresis was performed under the conditions of 60 mA for 35 min. The bands were visualized by the Chemidoc system at 365 nm.

Pooladanda V., Thatikonda S., Muvvala S.P., Devabattula G, & Godugu C. (2021). BRD4 targeting nanotherapy prevents lipopolysaccharide induced acute respiratory distress syndrome. International Journal of Pharmaceutics, 601, 120536.

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Immunoprecipitation and Western Blot Analysis

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These procedures were performed as previously described.^{53 (link)} In brief, cells were rinsed with PBS and lysed in Gold lysis buffer (GLB) containing 137 mM NaCl, 30 mM Tris buffer pH 8.0, 5 mM EDTA, 15% glycerol and 1% Triton X-100 with addition of protease and phosphatase inhibitors. After lysis, nuclei were pelleted and the supernatant pre-cleared. Incubation with antibody was performed overnight followed by precipitation of antibody-antigen complexes with protein A/G Sepharose beads. After rinsing, bound proteins were eluted in Laemmli buffer and boiled for 3–5 min. Finally, samples and corresponding total cell lysates were subjected to SDS-PAGE and transferred to immobilon. Next, membranes were blocked in 5% milk in TBS-T, incubated with primary and secondary HRP-antibody followed by SuperSignal West Dura chemiluminescence reagent. Finally, imaging was performed with a ChemiDoc system.

Ouyang H., Wu S., Li W., Grey M.J., Wu W, & Hansen S.H. (2023). p120 RasGAP and ZO-2 are essential for Hippo signaling and tumor-suppressor function mediated by p190A RhoGAP. Cell reports, 42(12), 113486.

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CDKN2A Expression Analysis

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The cells were transfected with either CDKN2A or a control plasmid for 48 h. After transfection, the proteins were extracted and separated using SDS-PAGE. The protein extract was transferred to a PVDF membrane and detected using a CDKN2A primary antibody. Antigen-antibody response was measured using the ChemiDoc system and band intensity was measured using Image J.

Tang M., Luo W., Zhou Y., Zhang Z, & Jiang Z. (2023). Anoikis-related gene CDKN2A predicts prognosis and immune response and mediates proliferation and migration in thyroid carcinoma. Translational Oncology, 40, 101873.

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