BP nanomaterials (BPQDs and BPNSs) were incubated with plasma at 4 °C for 4 h. The products were washed three times with 500 μl PBS to thoroughly remove unbound plasma protein and centrifuged again for 20 min at 15,000 × g at 4 °C to collect BP–corona complexes. After resuspension in PBS, the zeta potential and size of BP–corona complexes were measured on a Nano-ZS instrument (Malvern Instruments Limited) and DLS (Brookhaven BI-200SM). In addition, the zeta potential and size of BPQDs and BPNSs were also measured in PBS. All experiments were conducted at least twice to ensure the reproducibility of the results.
Bi 200sm
Manufactured by Brookhaven Instruments
Sourced in United States
The BI-200SM is a multi-angle light scattering (MALS) instrument manufactured by Brookhaven Instruments. It is designed to measure the angular dependence of scattered light from macromolecules and nanoparticles in solution. The BI-200SM can be used to determine the molar mass, size, and shape of these particles.
Automatically generated - may contain errors
Lab products found in correlation
Bi 9000at, by Brookhaven Instruments (7 mentions)
Zetapals, by Brookhaven Instruments (3 mentions)
Jem 2100, by JEOL (3 mentions)
D8 advance, by Bruker (2 mentions)
Uv 3600 spectrophotometer, by Shimadzu (2 mentions)
Rf 5301pc fluorescence spectrometer, by Shimadzu (2 mentions)
Nano z, by Malvern Panalytical (1 mentions)
Millex hv filter, by Merck Group (1 mentions)
Mfp 3d bio, by Digital Instruments (1 mentions)
Themis z, by Thermo Fisher Scientific (1 mentions)
Nano zs instrument, by Malvern Panalytical (1 mentions)
Escalab 250xi spectroscope, by Thermo Fisher Scientific (1 mentions)
Xcalibur, by Thermo Fisher Scientific (1 mentions)
Ht7700, by Bruker (1 mentions)
Jem f200, by JEOL (1 mentions)
Rf 6000, by Shimadzu (1 mentions)
Multimode 8, by Bruker (1 mentions)
5500 atomic force microscope, by Agilent Technologies (1 mentions)
Vetex70, by Bruker (1 mentions)
Zetasizer zs90, by Malvern Panalytical (1 mentions)
79 protocols using bi 200sm
1
Characterization of BP Nanomaterial-Protein Coronas
2
Micelle Stability in Salt Solutions
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To assess the stability of the micelles vs. salt, 200 μL micelle samples were prepared as described above and titrated with 5M NaCl in steps of 100 mM. Light scattering intensity was measured at a 90-degree angle using a Brookhaven BI-200SM (Brookhaven Instruments, Holtsville, NY, USA) system with a 637 nm laser at room temperature. Critical salt concentrations were classified as the point where no structure is seen in the autocorrelation function and scattering intensity drops below 5000 counts per second. +/−50 mM error bars are shown to reflect the finite step size used in titration.
3
Dynamic Light Scattering Measurements
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DLS measurements were obtained with a Brookhaven Instruments Corp.BI-200SM goniometer equipped with a BI-9000AT digital correlator using a solid-state laser (125 mW, λ = 532 nm). If not otherwise stated, measurements of scattered light were made at a scattering angle θ of 90°. The temperature was controlled with an accuracy of 0.1°C. Experiment duration was in the range of 5–20 minutes, and each experiment was repeated two or more times. CONTIN algorithm was used to fit the data. Measurements were carried out at 25°C.
4
Dynamic Light Scattering Analysis of Microgels
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Standard Laser Light Scattering (LLS) spectrometer (BI-200SM) equipped with a BI-9000 AT digital time correlator (Brookhaven Instruments, Inc.) and a Mini-L30 diode laser (30 mW, 637 nm) as the light source was used. All measurements were made in the 5.0 mM phosphate buffer solutions (PBS) of pH = 7.4. The very dilute composite microgel dispersions (10.0 μg mL−1) were passed through the Millipore Millex-HV filters (pore size 0.80 μm) to remove dust before LLS measurements. In Dynamic LLS (DLS), the Laplace inversion of each measured intensity–intensity time correlation function in a dilute dispersion can lead to a line-width distribution G(Γ). For a purely diffusive relaxation, Γ is related to the translational diffusion coefficient D by (Γ/q2)C→0,q→0 = D, so that G(Γ) can be converted to a translational diffusion coefficient distribution and the average hydrodynamic diameter (〈Dh〉) distribution by using the Stokes–Einstein equation, 〈Dh〉 = (kBT/3πη)/D, where kB, T, and η are the Boltzmann constant, absolute temperature, and solvent viscosity, respectively.74
5
Core-Shell Anionic Nano Microemulsion Protocol
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EXAMPLE 1
This example provides a core-shell structured anionic nano microemulsion system and a preparation method thereof. The main preparation steps and test results are as follows:
(1) In parts by weight, 5 parts of xylene, 45 parts of anionic Gemini surfactant of N,N,N′,N′-dodecyl tetrasubstituted diphenyl ether sulfonate, 15.5 parts of butanol, and 34.5 parts of water are weighed and put into reactor, and mixed under agitation at 300 rpm, until completely dissolved, and a homogeneous mixed solution is obtained.
(2) In parts by weight, 0.2 parts of the above homogeneous mixed solution and 99.8 parts of water were taken, and mixed under agitation at 300 rpm in a reactor, until completely dissolved, to obtain a core-shell structured anionic nano microemulsion system. The effective concentration thereof is 0.13%, the appearance is uniform and transparent, and it is stable for a long time.
(3) The initial average particle size of the microemulsion was determined to be 8.0 nm by dynamic light scattering (BI-200SM, Brookhaven) at 90°. After stabilization for 22 days, the average particle size was 152.8 nm (see
6
Anionic Nano Microemulsion Preparation and Characterization
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EXAMPLE 2
This example provides a core-shell structured anionic nano microemulsion system and a preparation method thereof. The main preparation steps and test results are as follows:
(1) In parts by weight, 10 parts of pyrrolidone, 50 parts of anionic Gemini surfactant of N,N,N′,N′-dodecyl tetrasubstituted diphenyl ether sulfonate, 20 parts of isopropanol, and 20 parts of water are weighed and put into reactor, and mixed under agitation at 300 rpm, until completely dissolved, and a homogeneous mixed solution is obtained.
(2) In parts by weight, 0.2 parts of the above homogeneous mixed solution and 99.8 parts of water were taken, and mixed under agitation at 300 rpm in a reactor, until completely dissolved, to obtain a core-shell structured anionic nano microemulsion system. The effective concentration thereof is 0.16%, the appearance is uniform and transparent, and it is stable for a long time.
(3) The initial average particle size of the microemulsion was determined to be 12.1 nm by dynamic light scattering (BI-200SM, Brookhaven) at 90°. After stabilization for 22 days, the average particle size was 267.0 nm (see
7
Core-Shell Anionic Nano Microemulsion Synthesis
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EXAMPLE 3
This example provides a core-shell structured anionic nano microemulsion system and a preparation method thereof. The main preparation steps and test results are as follows:
(1) In parts by weight, 5 parts of thiophene, 45 parts of anionic Gemini surfactant of N,N,N′,N′-dodecyl tetrasubstituted diphenyl ether sulfonate, 20 parts of ethanol, and 30 parts of water are weighed and put into reactor, and mixed under agitation at 300 rpm, until completely dissolved, and a homogeneous mixed solution is obtained.
(2) In parts by weight, 0.3 parts of the above homogeneous mixed solution, 98.7 parts of water and 1 part of NaCl were taken, and mixed under agitation at 300 rpm in a reactor, until completely dissolved, to obtain a core-shell structured anionic nano microemulsion system. The effective concentration thereof is 0.21%, the appearance is uniform and transparent, and it is stable for a long time.
(3) The initial average particle size of the microemulsion was determined to be 66.5 nm by dynamic light scattering (BI-200SM, Brookhaven) at 90°. After stabilization for 22 days, the average particle size was 285.6 nm (see
8
Dynamic and Static Light Scattering of SPI
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Dynamic and static light scattering of SPI solutions were determined by BI-200SM dynamic laser scattering system (Brookhaven Instruments, Holtsville, NY, USA). The helium-neon laser with 35 mW power and 633 nm wavelength was as the light source. The samples prepared for DLS and SLS were filtered through 0.45 μm Millipore filters into a precision cylindrical cell (quartz, diameter: 25 mm). Light scattering measurements were measured in the angular range of 30–120° for static measurements and 30–90° for dynamic measurements [40 (link)]. The refractive index increment was determined to be 0.185 mL/g for SPI in aqueous solution. The autocorrelation function was analyzed using CONTIN and NNLS software. All the light scattering measurements were conducted at 25 °C and controlled within 0.01 °C by water circulating apparatus.
9
Membrane Fraction Characterization by AFM
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To morphologically characterize the isolated membrane fraction, specimens were diluted 1:1,000 in PBS, and absorbed onto freshly cleaved mica sheets for 20 min. To provide a surface coated with formulations to a suitable density, the mica sheets were rinsed three times with deionized water and then dried with filter paper before detection. Surface morphology was examined under an atomic force microscope (Asylum Research MFP-3D-Bio; Digital Instruments, Santa Barbara, CA, USA) as described by Mu et al. (2014) (link). The particle size distribution of EPDELNs was evaluated using a Light Scattering System (Brookhaven BI-200SM) as previously described (Mu et al., 2014 (link)). Measurements were made in PBS at pH 7.0 at 25 °C after appropriate dilution of each EPDELN sample.
10
Polymer Particle Size Analysis via DLS
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The microscopic morphology of the polymers can be indicated by observing their particle size distribution. Dynamic light scattering (DLS) with a wide angle laser light scatterometer (BI-200SM, Brookhaven, Suffolk, NY, USA) is a common method to characterize particle size distribution by measuring the fluctuation of light intensity with time. Solutions of the drag reducers were prepared with different concentrations of brine. The test temperature was 25 °C, the laser module was a 532-Na light source, the detection angle was 90°, and CONTIN software was used for the final analysis of the data.
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