Cobalt chloride hexahydrate

Manufactured by Merck Group

Sourced in United States, India, Germany

Cobalt chloride hexahydrate is an inorganic chemical compound with the formula CoCl2·6H2O. It is a solid, crystalline material that appears as red or purple crystals. The compound is commonly used in various laboratory and industrial applications, including as a desiccant, an indicator, and a catalyst.

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Lab products found in correlation

Sodium borohydride, by Merck Group (5 mentions) Nickel chloride hexahydrate, by Merck Group (5 mentions) Milli q water, by Merck Group (4 mentions) Urea, by Merck Group (4 mentions) Sodium chloride (nacl), by Merck Group (4 mentions) Sodium hydroxide (naoh), by Merck Group (4 mentions) Ferric chloride, by Merck Group (3 mentions) Ethanol, by Merck Group (3 mentions) Chloroform, by Merck Group (3 mentions) Iron chloride hexahydrate, by Merck Group (3 mentions) Ethanol, by Xilong (3 mentions) Sodium hydroxide (naoh), by Xilong (3 mentions) Potassium permanganate, by Merck Group (2 mentions) Hydrogen peroxide, by Merck Group (2 mentions) Sodium nitrate, by Merck Group (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Hydrogen peroxide h2o2, by Merck Group (2 mentions) Sodium thiosulfate, by Merck Group (2 mentions) Sodium dodecyl sulfate (sds), by Merck Group (2 mentions) Copper sulfate pentahydrate, by Merck Group (2 mentions)

39 protocols using cobalt chloride hexahydrate

Synthesis of Cobalt Nanoparticles

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Analytical grade reagents were procured and used without further purification. Cobalt chloride hexahydrate (CoCl₂·6H₂O), sodium borohydride (NaBH₄), and ammonium hydroxide (NH₄OH) were procured from Merck Chemicals, India. The quaternary ammonium salts, tetramethylammonium hydroxide (TMAH, 25% (w/w), aqueous), tetraethylammonium hydroxide (TEAH, 35% (w/w), aqueous), and tetrabutylammonium hydroxide (TBAH, 25% (w/w), aqueous) were procured from Sigma-Aldrich Chemicals GmbH. In all the experiments, MilliQ water with a conductivity of σ = 0.0016 S/m was used and the temperature was maintained at 27 °C unless mentioned otherwise.

Deshmukh R., Mehra A, & Thaokar R. (2017). Formation and shape-control of hierarchical cobalt nanostructures using quaternary ammonium salts in aqueous media. Beilstein Journal of Nanotechnology, 8, 494-505.

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Synthesis of Graphene Oxide Derivatives

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In this study, ferric chloride (FeCl₃, 98%, Merck), ferrous chloride tetrahydrate (FeCl₂∙4H₂O, 98%, Merck), cobalt chloride hexahydrate (CoCl₂∙6H₂O, 98%, Merck), and nickel nitrate hexahydrate (Ni(NO₃)₂∙6H₂O, 98%, Merck) were utilized. Graphite flakes (99.5%, Sigma-Aldrich), sodium nitrate (NaNO₃, 99.9%, Merck), potassium permanganate (KMnO₄, 99.5%, Merck), hydrogen peroxide (H₂O₂, 30%, Merck), aqueous ammonia (NH₃(aq), 99.9%, Merck) and sulfuric acid (H₂SO₄, 97%, Merck) were also employed in this study. Reactive Red 66 (C₂₀H₁₅BrN₄Na₂O₈S₂, R66, a monoazo dye) and Reactive Red 120 (C₄₄H₂₄Cl₂N₁₄Na₆O₂₀S₆, R120, diazo dye) were sourced from Nordex International and D.Z.E Dye Company in the UK, respectively.

Parastar Gharehlar M., Sheshmani S., Nikmaram F.R, & Doroudi Z. (2024). Synergistic potential in spinel ferrite MFe2O4 (M = Co, Ni) nanoparticles-mediated graphene oxide: Structural aspects, photocatalytic, and kinetic studies. Scientific Reports, 14, 4625.

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Synthesis and Characterization of Chitosan-Based Materials

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Dimethyl sulfoxide (DMSO), ferric chloride hexahydrate (FeCl₃·6H₂O), cobalt chloride hexahydrate (CoCl₂·6H₂O), low molecular weight CH (product # 448 869, M_W = 50–190 kDa, degree of deacetylation [DD] = 75–85%), sodium acetate (CH₃COONa, NaAc), ethylene glycol, glacial acetic acid (CH₃COOH, 99%), ethanol, and hydrogen peroxide (H₂O₂, 30%) were purchased from Merck (Darmstadt, Germany). 3,3′,5,5′-tetramethylbenzidine (TMB), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), and the microbial culture media of Luria–Bertani (LB), Mueller–Hinton and blood agar were purchased from Sigma (Sigma-Aldrich, Germany). RPMI-1640 medium, fetal bovine serum (FBS), trypsin–EDTA solution, and antibiotic solution (penicillin–streptomycin) were obtained from Gibco (Invitrogen, Grand Island, NY).

Kajani A.A., Pouresmaeili A, & Kamali M. (2024). Facile one-pot synthesis of the mesoporous chitosan-coated cobalt ferrite nanozyme as an antibacterial and MRI contrast agent. RSC Advances, 14(24), 16801-16808.

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Spectrophotometric Determination of Cobalt

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Analytical-grade cobalt
chloride hexahydrate (CoCl₂·6H₂O), monosodium
succinate (C₄H₅O₄Na), sodium dodecyl
sulfate (NaC₁₂H₂₅SO₄), hydrazine
hydrate (N₂H₄), methyl red (MR), Eriochrome
Black-T (EBT), bromophenol blue (BPB), and malachite green (MG) as
organic dyes; potassium iodide (KI), potassium bromate (KB), sodium
thiosulfate (ST), benzoquinone (BQ) as scavengers; and ethanol and
acetone as solvents were purchased from Merck, India, and used without
further purification.

Sonkusare V.N., Chaudhary R.G., Bhusari G.S., Mondal A., Potbhare A.K., Mishra R.K., Juneja H.D, & Abdala A.A. (2020). Mesoporous Octahedron-Shaped Tricobalt Tetroxide Nanoparticles for Photocatalytic Degradation of Toxic Dyes. ACS Omega, 5(14), 7823-7835.

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Cobalt-Phosphate Complex Synthesis

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Cobalt chloride hexahydrate (CoCl₂.6H₂O), cobalt acetate tetrahydrate [Co(CH₃COO)₂.4H₂O)] and di-sodium hydrogen phosphate (Na₂HPO₄) anhydrous were purchased from Sigma-Aldrich (San Francisco, USA). Ammonia water (NH₄OH 28%) and CH₄N₂O were purchased from Merck (Darmstadt, Germany). In-house prepared deionized (DI) water was used throughout the experiment.

Anwar A., Numan A., Siddiqui R., Khalid M, & Khan N.A. (2019). Cobalt nanoparticles as novel nanotherapeutics against Acanthamoeba castellanii. Parasites & Vectors, 12, 280.

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Synthesis of Cobalt-Nickel Materials

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Several chemicals have been purchased from Sigma Aldrich Karachi Pakistan, including cobalt chloride hexahydrate (98%), nickel chloride hexahydrate (99.9%), urea (99%), ethanol (99%), potassium dihydrogen phosphate (98%), disodium hydrogen phosphate (98%), sodium chloride (99.5%), and sodium hydroxide (97%). All chemicals received are of high analytical quality and can be applied without further treatment. A deionized water solution was used to prepare growth precursors for the synthesis of the materials.

Mangrio S., Tahira A., Chang A.S., Mahar I.A., Markhand M., Shah A.A., Medany S.S., Nafady A., Dawi E.A., Saleem L.M., Mustafa E.M., Vigolo B, & Ibupoto Z.H. (2023). Advanced Urea Precursors Driven NiCo2O4 Nanostructures Based Non-Enzymatic Urea Sensor for Milk and Urine Real Sample Applications. Biosensors, 13(4), 444.

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Sodom Apple Milk Cobalt Extraction

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Chemical reagents such as cobalt chloride hexahydrate (CoCl₂·6H₂O), urea, and potassium hydroxide (KOH) were obtained from Sigma Aldrich Karachi Pakistan. The milk of the Sodom apple was collected from the mountains of Jamshoro, Sindh Pakistan. All the desired solutions were prepared in deionized water.

Bhatti A.L., Tahira A., Kumar S., Ujjan Z.A., Bhatti M.A., Kumar S., Aftab U., Karsy A., Nafady A., Infantes-Molina A, & Ibupoto Z.H. (2023). Facile synthesis of efficient Co3O4 nanostructures using the milky sap of Calotropis procera for oxygen evolution reactions and supercapacitor applications. RSC Advances, 13(26), 17710-17726.

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Cobalt Chloride and Cell Imaging

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Cobalt chloride hexahydrate (CoCl₂·6H₂O; Sigma-Aldrich; Merck KGaA) was dissolved in deionized water to obtain a 200 mmol/l CoCl₂ stock solution. The solution was further diluted with DMEM/F12 (Gibco; Thermo Fisher Scientific, Inc.) to achieve the desired concentration prior to use. Rhodamine phalloidin (Invitrogen; Thermo Fisher Scientific, Inc.) was dissolved in methanol to yield a stock concentration of 200 U/ml. The solution was diluted with PBS to achieve the staining solution required for subsequent experiments. DAPI (cat. no. D1306) was purchased from Invitrogen; Thermo Fisher Scientific, Inc. Mouse anti-human fascin-1 (cat. no. TA807305), rabbit anti-human HIF-1α (cat. no. ZA0552) and mouse anti-human β-actin (cat. no. TA-09) antibodies were purchased from Origene Technologies, Inc. Mouse anti-human HIF-1α antibody (cat. no. 79233) was purchased from Cell Signaling Technology, Inc. Matrix metalloproteinase (MMP)-2 (cat. no. 10373-2-AP) and MMP-9 (cat. no. 10375-2-AP) antibodies were purchased from ProteinTech Group, Inc. E-cadherin antibody (cat. no. 610181) was purchased from BD Biosciences. Vimentin antibody (cat. no. ab92547) was purchased from Abcam.

Bu M., Liu X., Liu X, & Xu W. (2019). Upregulation of fascin-1 is involved in HIF-1α-dependent invasion and migration of hypopharyngeal squamous cell carcinoma. International Journal of Oncology, 55(2), 488-498.

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Toxicity Evaluation of Chemicals

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Lithium chloride (Sigma), copper sulfate pentahydrate (Sigma), nickel (II) sulfate hexahydrate (Sigma), zinc sulfate (Merck), aluminum chloride hexahydrate (Sigma), cobalt chloride hexahydrate (Sigma), lead chloride (Sigma) and acrylamide (Sigma) were used for the toxicity tests. Cadmium chloride (Merck), arsenic trioxide (Merck), atrazine (EQ Laboratories), chlorpyrifos (EQ Laboratories), carbofuran (Chem Service), dimethoate (Chem Service), glyphosate (EQ Laboratories), and methoxychlor (Fluka) were provided by Taiwan Agricultural Chemicals and the Toxic Substances Research Institute, Council of Agriculture, Taiwan. Heavy metals, acrylamide, dimethoate and glyphosate were dissolved in sterile distilled water to make stock solutions, whereas other chemicals were either dissolved or diluted with dimethyl sulfoxide (DMSO) to the desired concentration as stock solutions.

Lee H.C., Lu P.N., Huang H.L., Chu C., Li H.P, & Tsai H.J. (2014). Zebrafish Transgenic Line huORFZ Is an Effective Living Bioindicator for Detecting Environmental Toxicants. PLoS ONE, 9(3), e90160.

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Synthesis of Metal Chalcogenide Nanomaterials

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Nickel sulfate hexahydrate (NiSO₄.6H₂O), lead(II) nitrate (Pb(NO₃)₂), cobalt chloride hexahydrate (CoCl₂.6H₂O), copper sulfate pentahydrate (CuSO₄·5H₂O), selenium (Se), zinc acetate dehydrate [Zn(CH₃COO)₂.2H₂O], thioacetamide (C₂H₅NS), urea (CH₄N₂O), acetic acid (C₂H₄O₂), cadmium acetate dehydrate [Cd(CH₃COO)₂.2H₂O], sulfur (S), potassium chloride (KCl), sodium sulfide (Na₂S), cadmium sulfate (CdSO₄·8/3H₂O), nitro tri-acetic acid (N(CH₂CO₂H)₃), sodium thiosulfate (Na₂S₂O₃), N-methyl-2-pyrrolidone (C₅H₉NO), polyvinylidene fluoride (PVDF), and Ni-foams were purchased from Sigma-Aldrich and used without further purification. TiO₂ paste (Ti-Nanoxide HT/SP) was supplied by Solaronix. The MWCNTs (Carbon Nano-Material Technology Co., Ltd) were used as received.

Muralee Gopi C.V., Ravi S., Rao S.S., Eswar Reddy A, & Kim H.J. (2017). Carbon nanotube/metal-sulfide composite flexible electrodes for high-performance quantum dot-sensitized solar cells and supercapacitors. Scientific Reports, 7, 46519.

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