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Centrifuge 5417r

Manufactured by Eppendorf
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Sourced in Germany, Italy, United States, China

The Centrifuge 5417R is a high-performance, refrigerated microcentrifuge designed for a wide range of laboratory applications. It features a maximum speed of 16,100 rpm and a maximum RCF of 21,382 x g. The centrifuge has a temperature range of -11°C to 40°C and can accommodate a variety of rotor types for different sample volumes and formats.

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Lab products found in correlation

Piercetm bca protein assay kit, by Thermo Fisher Scientific (3 mentions) Multi tube vortex mixer, by Allsheng (2 mentions) Bovine serum albumin (bsa), by Merck Group (2 mentions) Trizol, by Thermo Fisher Scientific (2 mentions) Concentrator plus, by Eppendorf (2 mentions) Thermomixer c, by Eppendorf (2 mentions) Vapor pressure osmometer, by Wescor (2 mentions) Maxq 4000, by Thermo Fisher Scientific (2 mentions) Protease inhibitor cocktail, by Roche (2 mentions) Tissuelyser lt, by Qiagen (2 mentions) Precellys evolution, by Bertin Technologies (2 mentions) Nanodrop one, by Thermo Fisher Scientific (2 mentions) Infinite m200 pro, by Tecan (2 mentions) Multiskan go, by Thermo Fisher Scientific (2 mentions) Zetasizer nano z, by Malvern Panalytical (1 mentions) Pluronic acid, by Merck Group (1 mentions) Hplc 1100 series system, by Hewlett-Packard (1 mentions) Vacutainer, by BD (1 mentions) Biomate spectrophotometer, by Thermo Fisher Scientific (1 mentions) Xs105 dualrange, by Mettler Toledo (1 mentions)

103 protocols using centrifuge 5417r

1

Quantifying Droplet Deposition using Colorimetry

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The droplet deposition was obtained by measuring the Allura red content on the filter paper. About 5 ml of distilled water was added to each zip lock bag with filter paper and washed with a small shaker for 10 min, then centrifuged at 4,000 rpm for 5 min (Eppendorf 5417R Centrifuge, Eppendorf Co., Ltd., Hamburg, Germany). The absorbance value (Yi) was determined using the Infinite 200Pro ELISA instrument (Tecan, Meilen, Switzerland) at 510 nm. The Yi was then converted to mass concentration and Xi according to the linear regression equation of the Allura red standard solution (Y = 0.0238X + 0.0431, R2 = 0.997). The deposition amount per unit area was calculated using Equation 3.
where A is droplet deposition per unit area, Xi is the mass concentration of eluent, V is the volume of the added eluent, S is the area of droplet collector.
Liu Y., Xiao Q., Han X., Zeeshan M., Fang Z, & Dou Z. (2022). Effect of aerial application of adjuvants on pepper defoliant droplet deposition and efficacy of defoliation sprayed by unmanned aerial vehicles. Frontiers in Plant Science, 13, 917462.
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2

Soy Protein Dispersion Characterization

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Soy protein dispersions were prepared by adding SPI powder to deionised (DI) water at 5%, w/w, total solids (20 °C). The suspension was agitated using a magnetic stirrer (Dynalon Stir Bar Kit, VWR, Blanchardstown, Dublin, Ireland) at 350 rpm for 1 h. After 1 h of stirring, these dispersions were centrifuged at 800× g for 10 min, and the supernatant was removed. The initial pH of the dispersion was pH 6.9. Then, the pH was adjusted using HCl or NaOH, ranging from pH 2.0 to 9.0. Then, the solids content was diluted to a final concentration of 0.002%, w/w, using DI water and an Eppendorf 5417R centrifuge (Eppendorf AG, 22331 Hamburg, Germany). Samples were measured using a Zetasizer Nano ZS (Malvern Instruments Ltd., Worcestershire, UK). All measurements were carried out at 25 °C, in triplicate, in 10 mm polystyrene cuvettes using a refractive index of 1.45.
O′Flynn T.D., Hogan S.A., Daly D.F., O′Mahony J.A, & McCarthy N.A. (2021). Rheological and Solubility Properties of Soy Protein Isolate. Molecules, 26(10), 3015.
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3

Pigment Analysis of Microalgae Cultures

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Triplicate cultures were harvested during the exponential phase when FV/FM was maximum for each temperature condition. Cultures (50 mL) were subjected to centrifugation in the presence of 0.01% (v/v) pluronic acid (Sigma-Aldrich, Germany) at 4°C, 14,000 × g for 7 min, using an Eppendorf 5804R (Eppendorf, France). Pellets were resuspended and transferred to 1.5 ml Eppendorf tubes and centrifuged at 4°C, 17,000 × g for 2 min using an Eppendorf 5417R centrifuge (Eppendorf, France). Once the supernatant was removed samples were stored at −80°C until further analysis. Pigment content was subsequently assessed using calibrated high-performance liquid chromatography (HPLC 1100 Series System, Hewlett Packard, St Palo Alto, CA), as previously described (Six et al., 2005 (link)).
Ferrieux M., Dufour L., Doré H., Ratin M., Guéneuguès A., Chasselin L., Marie D., Rigaut-Jalabert F., Le Gall F., Sciandra T., Monier G., Hoebeke M., Corre E., Xia X., Liu H., Scanlan D.J., Partensky F, & Garczarek L. (2022). Comparative Thermophysiology of Marine Synechococcus CRD1 Strains Isolated From Different Thermal Niches in Iron-Depleted Areas. Frontiers in Microbiology, 13, 893413.
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4

Blood Sample Collection and Processing

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In the morning, after a minimum of 12 h of fasting, blood samples were collected in commercially available covered test tubes without any anticoagulant and, in order to prevent blood clotting, in lavender topped K2EDTA BD vacutainers (Becton-Dickinson). Blood samples collected in K2EDTA tubes were used to perform a complete blood count (CBC).
For each patient, a sample of blood was also collected in black capped BD ESR (Becton-Dickinson) tubes. Plasma and blood cell fractions were separated by centrifugation of blood also collected in vacutainers containing K2EDTA at 2,000 x g, for 10 min, at 4˚C (5417R Eppendorf centrifuge; Eppendorf AG). Immediately after separation, the plasma was aliquoted in Eppendorf tubes and stored under proper conditions (at -80˚C, avoiding repeated freezing/refreezing cycles) until determination of several oxidative stress markers. The sediment was processed to obtain a hemolysate that was preserved for further analyses.
Serum was separated by centrifugation of blood collected in red topped BD vacutainers (Becton-Dickinson) at 1,000 x g for 10 min, after which it was allowed to clot for 20 min at room temperature, and used for the measurement of several inflammatory markers and biochemical parameters.
Pomacu M.M., Trașcă M.D., Pădureanu V., Bugă A.M., Andrei A.M., Stănciulescu E.C., Baniță I.M., Rădulescu D, & Pisoschi C.G. (2021). Interrelation of inflammation and oxidative stress in liver cirrhosis. Experimental and Therapeutic Medicine, 21(6), 602.
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5

Plasma Separation and Oxidative Stress Analysis

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The blood samples were collected in the morning after 12 h of fasting by an experimented phlebotomist using EDTA (ethylene-diamine-tetraacetic acid) tube collection, in order to prevent blood clotting. Plasma and red blood cell (RBC) fractions were separated by centrifugation at 3600 rpm at 4 °C for 10 min (5417R Eppendorf centrifuge). The sediments were divided from plasma immediately after centrifugation and oxidative stress markers were analyzed in the plasma.
Padureanu R., Albu C.V., Mititelu R.R., Bacanoiu M.V., Docea A.O., Calina D., Padureanu V., Olaru G., Sandu R.E., Malin R.D, & Buga A.M. (2019). Oxidative Stress and Inflammation Interdependence in Multiple Sclerosis. Journal of Clinical Medicine, 8(11), 1815.
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6

Glutathione S-Transferase Activity Assay

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GST activity was determined according to Habig et al. [27 (link)] using 1-chloro 2,4-dinitrobenzene as a substrate with some modifications. The samples were thawed on ice, homogenized, and centrifuged at 9205 rpm for 15 min at 4 °C (5417R Eppendorf centrifuge, Germany). After removing the supernatant, the pellets were resuspended in 200 μL of DPBS buffer and centrifuged again to collect as much enzyme as possible. The combined supernatant (from two centrifugation times) was used for the GSTs and protein assay. The substrate (reaction solution) was a mixture of 100 mM DPBS buffer (pH 6.5), 200 mM GSH and 100 mM CDNB. The reaction was started by mixing 0.95–0.98 mL of the reaction mixture with 0.02–0.05 mL of samples, and the absorbance was measured every minute (for 8 min) at 340 nm using a Thermo ScientificTM Biomate spectrophotometer. A blank sample containing 1 mL of the substrate was included. The specific activities of GSTs were calculated and expressed as nmoles of GSH-CDNB conjugate formed/min/mg protein.
Ngo H.T., Nguyen T.D., Nguyen T.T., Le T.T, & Nguyen D.Q. (2022). Adverse Effects of Toxic Metal Pollution in Rivers on the Physiological Health of Fish. Toxics, 10(9), 528.
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7

Fractionation of Nanoparticle Dispersions

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Separation of the parent dispersion of nanoparticle in fractions of different size was achieved by successive centrifugations. Figure 1 summarizes the conditions of centrifugation used and the different fractions that were prepared during this fractionation procedure. During the procedure, the weight of each fraction was measured using a balance Mettler Toledo XS105 Dual range to achieve a mass balance of the distribution of the different types of nanoparticles isolated in each fraction.
The different fractions were stored at +4°C until use.
Figure 1: Scheme of experimental conditions used to fractionate the parent dispersion (P). Fractions F1, F2, F3 and F4 kept for further analysis were underlined with the color code used in the different figures. Centrifugation 1 was performed with a Centrifuge 5804R Eppendorf. Centrifugations 2 and 3 were performed using a 5417R Eppendorf centrifuge. Volumes were 50 mL per tube for centrifugation 1 and 1 mL distributed in microcentrifuge tubes for centrifugations 2 and 3.
Eleamen Oliveira E., Barendji M, & Vauthier C. (2020). Understanding Nanomedicine Size and Biological Response Dependency: What Is the Relevance of Previous Relationships Established on Only Batch-Mode DLS-Measured Sizes?. Pharmaceutical research, 37(8).
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8

Sphingomyelin Nanosystems Incorporate UroGm

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Sphingomyelin nanosystems incorporating the modified Uroguanylin (UroGm) were prepared by ethanol injection technique. Briefly, UroGm was dissolved in water at a concentration of 0.5 mg/mL. On the other hand, oleic acid and sphingomyelin were dissolved in ethanol at a concentration of 200 mg/mL and 40 mg/mL respectively. Subsequently, 50 μL of the oily phase (composed by 2.5 mg of oil and 0.5 mg of surfactant) were injected into 450 µL of ultrapure water (containing the appropriate quantity of UroGm) under continuous magnetic stirring and nanosystems were spontaneously formed. Increasing amounts of UroGm were added to the formulation in order to explore the maximum loading capacity (data not shown) establishing a final amount of 10 µg of UroGm per formulation as the best condition. Formulations were then isolated by centrifugation (20,000 RFC for 45 min at 15 °C) using an Eppendorf 5417R centrifuge (Eppendorf, Germany) to purify the nanosystems.
Bouzo B.L., Lores S., Jatal R., Alijas S., Alonso M.J., Conejos-Sánchez I, & de la Fuente M. (2021). Sphingomyelin nanosystems loaded with uroguanylin and etoposide for treating metastatic colorectal cancer. Scientific Reports, 11, 17213.
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9

Plasma Extraction for Mass Spectrometry

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Blood samples were collected and centrifuged (5417R centrifuge, Eppendorf AG, Hamburg, Germany) at 835× g for 10 min to separate the upper plasma. Then, 50 μL of plasma was mixed with 200 μL of methanol (containing dalbavancin B0-D8 isotope internal standard solutions at a concentration of 100 ng/mL), after which the solution was swirled with a multi-tube vortex mixer (Allsheng, Hangzhou, China) for 10 min and centrifuged at 12,000× g for 10 min. To obtain a cleaner supernatant, it was further centrifuged at 12,000× g for 5 min before sample injection.
Zhu D., Ping L., Hong Y., Shen J., Weng Q, & He Q. (2020). Simultaneous Quantification and Pharmacokinetic Study of Five Homologs of Dalbavancin in Rat Plasma Using UHPLC-MS/MS. Molecules, 25(18), 4100.
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10

High-resolution mass spectrometry protocol

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A Triple TOF® 6600 high-resolution mass spectrometry system (AB SCIEX, USA) equipped with an ACQUITY UPLC I-Class ultrahigh-performance liquid system (Waters, USA) and Analyst® TF data acquisition software (AB SCIEX, USA) was used. We used a 5417R centrifuge (Eppendorf, Germany); Vortex Mixer T1 vortex oscillator (Titan SCIENTIFIC LAB); TIMI-10K micromini centrifuge (Titan SCIENTIFIC LAB); and a Labconco centrifugal concentrator (Labconco, USA). Ultrapure water (18.2 MΩ•cm) was prepared using a Milli-Q purified water system (Merck Millipore, USA).
Bu Q., Zhang J., Guo X., Feng Y., Yan H., Cheng W., Feng Z, & Cao M. (2022). The antidepressant effects and serum metabonomics of bifid triple viable capsule in a rat model of chronic unpredictable mild stress. Frontiers in Nutrition, 9, 947697.
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