Mirvana rna extraction kit

Manufactured by Thermo Fisher Scientific

Sourced in United States

The MiRVana RNA extraction kit is a product developed by Thermo Fisher Scientific for the extraction and purification of RNA from various biological samples. The kit utilizes a specialized lysis and binding solution to capture RNA, followed by washing and elution steps to obtain purified RNA for downstream applications.

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Lab products found in correlation

Nanodrop spectrophotometer, by Thermo Fisher Scientific (3 mentions) Hiseq 1500, by Illumina (3 mentions) Reverse transcription kit, by Thermo Fisher Scientific (2 mentions) Biorad cfx96 thermocycler system, by Bio-Rad (2 mentions) Abi steponeplus machine, by Thermo Fisher Scientific (2 mentions) Trizol reagent, by Thermo Fisher Scientific (2 mentions) Nanodrop 2000c, by Thermo Fisher Scientific (2 mentions) Anti 5mc, by Abcam (2 mentions) Hybond n membrane, by GE Healthcare (2 mentions) Chemidoc imaging system, by Bio-Rad (2 mentions) Mirvana lysis buffer, by Thermo Fisher Scientific (1 mentions) 2100 bioanalyzer, by Agilent Technologies (1 mentions) Facsaria, by BD (1 mentions) Humanht 12 v4, by Illumina (1 mentions) Mrna seq library prep kit, by Illumina (1 mentions) Turbo dnase free kit, by Thermo Fisher Scientific (1 mentions) Paxgene blood rna kit, by Qiagen (1 mentions) Experion, by Bio-Rad (1 mentions) Truseq rna libraries, by Illumina (1 mentions) Hiseq 1500 platform, by Illumina (1 mentions)

24 protocols using mirvana rna extraction kit

Phenotypic Characterization of B Cell Subsets

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Example 19

Frozen vials of 10×10⁶PBMCs are thawed and washed before staining with Pacific Blue labeled anti-CD3 (UCHT1), Pacific Blue labeled anti-CD14 (M5E2), FITC labeled anti-CD19 (HIB19), PE-Cy5 labeled antiCD10 (HI10a), PE labeled anti-CD27 (M-T271), and APC labeled anti-CD21 (B-ly4), all from BD Biosciences. Sorts are performed on a high speed BD FACSAria into miRVana lysis buffer (Ambion). Immature B cells, exhausted tissue-like memory, activated mature B cells, resting memory B cells, and short-lived peripheral plasmablasts are stained using previously described markers³¹. Total RNA from the cells is then extracted using the miRVana RNA extraction kit (Ambion) according to manufacturer's instructions and quantitated on a 2100 Bioanalyzer (Agilent).

US11142565B2. Broadly neutralizing anti-HIV-1 antibodies that bind to an N-glycan epitope on the envelope (2021-10-12). ABVITRO LLC [US]. Inventors: Francois Vigneault [US], Adrian Wrangham Briggs [US], Stephen J. Goldfless [US], Sonia Timberlake [US].

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Transcriptomic profiling of purified cells

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After mRNA extraction from the purified cells (mirVana RNA extraction kit, Ambion), whole-genome transcriptional profiling was performed using Illumina Human HT-12 V4 microarrays according to standard protocols [15 (link)]. Data retrieved from the Illumina platform were background corrected, and quantile normalized as previously described [16 (link)].

Ouyang Z., Buzon M.J., Zheng L., Sun H., Yu X.G., Bosch R.J., Mellors J.W., Eron J.J., Gandhi R.T, & Lichterfeld M. (2015). Transcriptional Changes in CD8+ T Cells During Antiretroviral Therapy Intensified With Raltegravir. Open Forum Infectious Diseases, 2(2), ofv045.

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Growth and RNA Extraction in H. salinarum

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H. salinarum NRC-1 was grown in CM media, in a water bath incubator at 37°C with agitation of 125 r.p.m. Reference samples were cultured under standard growth conditions [26] (link), at mid-log phase (OD600≈0.5). Small RNAs for RNA-seq libraries and Total RNAs for dRNA-seq libraries and northern blot experiments were isolated using the MirVana RNA extraction kit (Ambion).

Zaramela L.S., Vêncio R.Z., ten-Caten F., Baliga N.S, & Koide T. (2014). Transcription Start Site Associated RNAs (TSSaRNAs) Are Ubiquitous in All Domains of Life. PLoS ONE, 9(9), e107680.

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Transcriptome Profiling of Landrace Boars

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Expression analysis based ion RNAseq data was performed as previously described [28] (link). Ten tissues from two unrelated one year old Landrace boars were included in the study. Hence, total RNA was extracted from heart, spleen, liver, kidney, lung, musculus longissimus dorsi, occipital cortex, hypothalamus, frontal cortex, and cerebellum employing the mirVana™ RNA extraction kit (Ambion) according to manufactures protocol, yielding a total of 20 samples. RNA integrity of the individual RNA samples was assessed on a 2% agarose gel. Library preparation was performed using the mRNA-seq library prep kit from Illumina [28] (link). Mapping and assembly of fragments was carried out as described previously [28] (link). Relative abundance of each transcript for each animal for all tissues in the unit of fragments per kilobase of exon per million fragments mapped (FPKM) were estimated.

Larsen K., Momeni J., Farajzadeh L, & Bendixen C. (2014). Porcine SLITRK1: Molecular cloning and characterization. FEBS Open Bio, 4, 872-878.

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Wheat Leaf Transcriptome Profiling

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An ad-hoc field survey was conducted during the wheat growing season from May to July, from, 2019 to, 2021 in major wheat growing counties of Kansas. A total of 84 samples exhibiting yellow discoloration or mosaic patterns were collected from the newest wheat leaf taken between jointing to the soft dough stage from 47 different counties of Kansas. Leaf tissue of each sample was stored at - 20°C until the tissue could be processed for RNA extraction. Total RNA was extracted using the mirVana RNA extraction kit (Ambion Catalog number: AM1560, Thermo Fisher Scientific, MA, USA) from 200 mg of tissue following the company’s instructions. RNA concentration was measured by NanoDrop spectrophotometer (NanoDrop Technologies, Rockland, DE, USA). Only samples with concentrations ranging from 100-120 ng/ul and 260/280 values between 1.8-2.0 were used in library preparation. Seven µg of total RNA was treated with 1 µl of DNase using Turbo DNase-Free ™ kit (AM, 1907, Ambion^®, Thermo Fisher, MA, USA) in a 50 µl reaction volume according to the manufacturer’s instruction.

Ranabhat N.B., Fellers J.P., Bruce M.A, & Rupp J.L. (2023). Brome mosaic virus detected in Kansas wheat co-infected with other common wheat viruses. Frontiers in Plant Science, 14, 1096249.

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Deep RNA Sequencing of Nucleated Cells

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Total RNA from the nucleated cells was extracted using either the MirVana RNA extraction kit (Invitrogen) or PAXgene Blood RNA Kit (Qiagen). Illumina TruSeq RNA Libraries were prepared from 1 μg total RNA. High-throughput (6.2 GB), paired-end (2 × 101 bp), deep sequencing coverage (104×) RNA-Seq using Illumina’s TruSeq technology was performed on the Illumina HiSeq 1500 (CMH Genetics Research Core Lab). The mRNA-Seq data are available at the Gene Expression Omnibus under the dataset, GSE143780 (https://www.ncbi.nlm.nih.gov/geo/).

Tu L.N., Hsieh L., Kajimoto M., Charette K., Kibiryeva N., Forero A., Hampson S., Marshall J.A., O’Brien J., Scatena M., Portman M.A., Savan R., Benner C., Aliseda A., Nuri M., Bittel D., Pastuszko P, & Nigam V. (2021). Shear stress associated with cardiopulmonary bypass induces expression of inflammatory cytokines and necroptosis in monocytes. JCI Insight, 6(1), e141341.

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Total RNA Extraction and Illumina Sequencing

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Total RNA from the nucleated cells was extracted using a MirVana RNA extraction kit (Invitrogen). The concentration and quality of total RNA for each sample were quantified and evaluated by spectrophotometry (Epoch; Thermo Scientific) and automated chip electrophoresis analyses (Experion, BioRad), respectively. Each sample met the quality standards of a 260/280 ratio >2.0 and an RNA integrity number >9. Illumina TruSeq RNA libraries were prepared from 1 μg of total RNA and sequenced to generate 50 base pair single‐end reads using a HiSeq 1500 (Illumina) at the Children's Mercy OMICs Research Core Lab (Kansas City, MO).

Hsieh L., Tu L.N., Paquette A., Sheng Q., Zhao S., Bittel D., O’Brien J., Vickers K., Pastuszko P, & Nigam V. (2022). microRNA Expression Levels Change in Neonatal Patients During and After Exposure to Cardiopulmonary Bypass. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 11(17), e025864.

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Total RNA Extraction and Sequencing

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Total RNA from the nucleated cells was extracted using either the MirVana RNA extraction kit (Invitrogen). The concentration and quality of total RNA for each sample were quantified and evaluated by spectrophotometry (Epoch; Thermo Scientific) and automated chip electrophoresis analyses (Experion, BioRad) respectively. Each sample met the quality standards of a 260/280 ratio > 2.0 and a RNA integrity number (RIN) > 9. Illumina TruSeq RNA libraries (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
The copyright holder for this preprint this version posted August 13, 2021. ; https://doi.org/10.1101/2021.08.13.454953 doi: bioRxiv preprint were prepared from 1 μg total RNA and sequenced to generate 50 base pair single-end reads using the the HiSeq 1500 (Illumina, USA) at the Children's Mercy OMICs Research Core Lab (Kansas City, MO).

Hsieh L., Tu L.N., Paquette A.G., Kibiryeva N., Marshall J., Bittel D.C., O’Brien J.E., Vickers K.C., Pastuszko P., & Nigam V. (2021). microRNA Expression Levels Change in Neonatal Patients During and After Exposure to Cardiopulmonary Bypass.

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Striatal Total RNA Sequencing

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Total RNA from the striatum was extracted using the MirVana RNA extraction kit (Invitrogen, Carlsbad, Calif). The concentration and quality of total RNA for each sample were quantified and evaluated by spectrophotometric (Epoch; Thermo Scientific, Waltham, Mass) and automated chip electrophoresis analyses (Experion, BioRad, Hercules, Calif), respectively. Each sample met our quality standards of a 260/280 ratio greater than 2.0 and a RNA integrity number greater than 9. Illumina TruSeq RNA Sample Preparation Kit was used to generate cDNA libraries from 1 mg total RNA and sequenced on a HiSeq 1500 platform (Illumina, San Diego, Calif) by the Children's Mercy OMICS Research Core Lab, Kansas City, Missouri.

Tu L.N., Timms A.E., Kibiryeva N., Bittel D., Pastuszko A., Nigam V, & Pastuszko P. (2019). Transcriptome profiling reveals activation of inflammation and apoptosis in the neonatal striatum after deep hypothermic circulatory arrest. The Journal of thoracic and cardiovascular surgery, 158(3).

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RNA Extraction and Real-Time PCR Analysis

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RNA was extracted from cells in vitro using a TRIzol reagent (Thermo Fisher). Tumor cell RNA was extracted using the mirVana RNA extraction kit (Life Technologies). Briefly, tumors were collected and snap frozen, then processed in 300 μL of lysis buffer using homogenization. RNA was then extracted as directed by the kit protocol.
All RNA samples were quantified, and reverse transcription was performed with 2 μg of RNA using qSCRIPT cDNA SuperMix (Quantabio, Beverly, MA, USA). Real-time PCR was performed using primer sets specific for each gene (obtained from Origene, Rockland, MD, USA) and the SYBR^® Green PCR Master Mix (Life Technologies). L32 (60S ribosomal gene) was used to normalize expression across samples.

Konen J.M., Rodriguez B.L., Fradette J.J., Gibson L., Davis D., Minelli R., Peoples M.D., Kovacs J., Carugo A., Bristow C., Heffernan T, & Gibbons D.L. (2019). Ntrk1 Promotes Resistance to PD-1 Checkpoint Blockade in Mesenchymal Kras/p53 Mutant Lung Cancer. Cancers, 11(4), 462.

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