Biomedical grade chitosan (average molecular weight of 5,000 kDa, degree of deacetylation 95 %) was supplied by Seafresh Chitosan (Lab) Company (Thailand). Phthalic anhydride and succinic anhydride were purchased from Fluka Chemical Company (Switzerland). Poly (ethylene glycol) methyl ethers (mPEG, average molecular weight of 5,000 Da) were obtained from Aldrich Chemical Company (USA). 1-Ethyl-3-(3 ´-dimethylaminopropyl)carbodiimide (EDC) was purchased from Tokyo Chemical Industry (Japan). 1-Hydroxybenzotriazole monohydrate (HOBt) was obtained from BDH Laboratory Supplies (UK). Dichloromethane and hexane were purchased from Samchai Chemical Company (Thailand). Ethyl acetate, chloroform, dimethylsulfoxide (DMSO), dimethylformamide (DMF), ethanol and methanol were purchased from Lab Scan Analytical Sciences (Thailand). Potassium dihydrogen phosphate (KH2PO4) was obtained from Ajax Finechem (Australia). All other reagents were of analytical grade and deionized (DI) water was used throughout the study.
1 ethyl 3 3 dimethylaminopropyl carbodiimide edc
Manufactured by Tokyo Chemical Industry
Sourced in Japan
1-Ethyl-3-(3'-dimethylaminopropyl)carbodiimide (EDC) is a water-soluble carbodiimide reagent commonly used in various chemical coupling reactions, particularly in peptide synthesis and protein modification applications.
Automatically generated - may contain errors
Lab products found in correlation
N hydroxysuccinimide, by Tokyo Chemical Industry (2 mentions)
Porcine gelatin, by Merck Group (1 mentions)
Furfurylamine, by Merck Group (1 mentions)
N hydroxysuccinimide nhs, by Tokyo Chemical Industry (1 mentions)
Dialysis membrane tube, by Thermo Fisher Scientific (1 mentions)
Triisopropylsilane, by Tokyo Chemical Industry (1 mentions)
N n dimethylformamide (dmf), by Nacalai Tesque (1 mentions)
Dichloromethane dcm, by Nacalai Tesque (1 mentions)
Cholesteryl hemisuccinate chems, by Merck Group (1 mentions)
α cyano 4 hydroxycinnamic acid, by Tokyo Chemical Industry (1 mentions)
Ez link nhs peg4 biotin, by Thermo Fisher Scientific (1 mentions)
N hydroxysuccinimide, by Fujifilm (1 mentions)
Boric acid, by Nacalai Tesque (1 mentions)
4 2 hydroxyethyl 1 piperazineethanesulfonic acid hepes, by Dojindo Laboratories (1 mentions)
Ethylenediaminetetraacetic acid edta, by Dojindo Laboratories (1 mentions)
3 aminopropyltriethoxysilane aptes, by Merck Group (1 mentions)
Cover glass, by Matsunami (1 mentions)
4 dimethylaminopyridine, by Merck Group (1 mentions)
Dimethylformamide, by Merck Group (1 mentions)
N hydroxysuccinimide, by Merck Group (1 mentions)
7 protocols using 1 ethyl 3 3 dimethylaminopropyl carbodiimide edc
1
Chitosan-based hydrogel synthesis
2
Synthesis and Characterization of Furfurylated Gelatin
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F-gelatin
was synthesized and characterized as previously reported.28 (link) Porcine gelatin (4 g, Sigma-Aldrich) was dissolved
in 300 mL of milli-Q water. N-Hydroxysuccinimide
(NHS) (3.9 g, Tokyo Chemical Industry, Tokyo, Japan) and 1-ethyl-3-(3-(dimethylamino)propyl)carbodiimide
(EDC) (4.8 g, Tokyo Chemical Industry), dissolved in 100 mL of milli-Q
water, and furfurylamine (1.5 g, Sigma-Aldrich) were added slowly
to the gelatin solution, and then the solution was stirred at 40 °C
for 24 h. After the reaction, the solution was filtrated and then
dialyzed using a dialysis membrane with a molecular weight cutoff
of 3500 Da (Spectrum Laboratories, Rancho Dominguez, CA) against water
for 3 days in dark conditions. The f-gelatin was recovered by lyophilization
and was used for further studies.
was synthesized and characterized as previously reported.28 (link) Porcine gelatin (4 g, Sigma-Aldrich) was dissolved
in 300 mL of milli-Q water. N-Hydroxysuccinimide
(NHS) (3.9 g, Tokyo Chemical Industry, Tokyo, Japan) and 1-ethyl-3-(3-(dimethylamino)propyl)carbodiimide
(EDC) (4.8 g, Tokyo Chemical Industry), dissolved in 100 mL of milli-Q
water, and furfurylamine (1.5 g, Sigma-Aldrich) were added slowly
to the gelatin solution, and then the solution was stirred at 40 °C
for 24 h. After the reaction, the solution was filtrated and then
dialyzed using a dialysis membrane with a molecular weight cutoff
of 3500 Da (Spectrum Laboratories, Rancho Dominguez, CA) against water
for 3 days in dark conditions. The f-gelatin was recovered by lyophilization
and was used for further studies.
3
Botulinum Toxin-Hyaluronate Conjugate Synthesis
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Hyaluronate (HA) was purchased from Lifecore Co. (Chaska, MN). Botulinum toxins (BTX, Meditox, Ochang, Korea) were kindly gifted from Dr. Jeesoo An at the Wellman Center for Photomedicine in Massachusetts General Hospital. 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) was obtained from Tokyo Chemical Industry (Tokyo, Japan). N-hydroxysulfosuccinimide (sulfo-NHS), phosphate buffered saline (PBS), diaminohexane (DAH) and hydroxylbenzotriazole (HOBt) were purchased from Sigma (St. Louis, MO). Dialysis membrane tube was obtained from Thermo Scientific Co. (Waltham, MA).
4
Solid-Phase Peptide Synthesis Reagents
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3-[Bis(dimethylamino)methyliumyl]-3H-benzotriazol-1-oxide hexafluorophosphate (HBTU), 1-hydroxybenzotriazole hydrate (HOBt·H2O), triisopropylsilane (TIS), α-cyano-4-hydroxycinnamic acid (CHCA), Kaiser test kit (phenol/ethanol, ninhydrin/ethanol, potassium cyanide/pyridine), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC), and N-hydroxysuccinimide (NHS) were purchased from Tokyo Chemical Industry (TCI, Tokyo, Japan). Dichloromethane (DCM) and N,N-dimethylformamide (DMF) were purchased from Nacalai Tesque Inc (San Diego, CA, USA). 1,2-Distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (DSPE-mPEG2000; DSPE-PEG) and egg L-α-phosphatidylcholine (PC) were purchased from Avanti Polar Lipid Inc. (Alabaster, AL, USA). In addition, cholesteryl hemisuccinate (CHEMS) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Piperidine (PPD) was purchased from Watanabe Chemical Industries (Hiroshima, Japan).
5
Biotin-Avidin Surface Functionalization
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NeutrAvidin
and EZ-Link NHS-PEG4-biotin were
purchased from Thermo Fisher Scientific (Waltham, MA). Biotinylated
DNA and fluorescent-tagged oligonucleotides were purchased from Eurofin
Genomics (Tokyo, Japan). Manicure was purchased from Shiseido (Tokyo,
Japan). 3-Aminopropyltriethoxysilane (APTES) was purchased from Sigma
Aldrich (St. Louis, MO). Boric acid was purchased from Nacalai Tesque
(Kyoto, Japan). 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid
(HEPES) and ethylenediaminetetraacetic acid (EDTA) were purchased
from Dojindo (Kumamoto, Japan). A cover glass (18 mm × 18 mm,
no. 1S, 0.175 ± 0.015 mm thickness) and a slide glass (76 mm
× 26 mm, S-0314 Neo no. 2, 1.0–1.2 mm thickness) were
purchased from Matsunami Glass Industries (Osaka, Japan). 1-Ethyl-3-[3-dimethylaminopropyl]carbodiimide
(EDC) was purchased from Tokyo Chemical Industry (Tokyo, Japan). N-Hydroxysuccinimide (NHS) was purchased from Wako Pure
Chemical Industries (Osaka, Japan). All of the other reagents were
purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan),
unless otherwise specified, and used without further purification.
Type 1 ultrapure water (Milli-Q) was used in all of the experiments.
and EZ-Link NHS-PEG4-biotin were
purchased from Thermo Fisher Scientific (Waltham, MA). Biotinylated
DNA and fluorescent-tagged oligonucleotides were purchased from Eurofin
Genomics (Tokyo, Japan). Manicure was purchased from Shiseido (Tokyo,
Japan). 3-Aminopropyltriethoxysilane (APTES) was purchased from Sigma
Aldrich (St. Louis, MO). Boric acid was purchased from Nacalai Tesque
(Kyoto, Japan). 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid
(HEPES) and ethylenediaminetetraacetic acid (EDTA) were purchased
from Dojindo (Kumamoto, Japan). A cover glass (18 mm × 18 mm,
no. 1S, 0.175 ± 0.015 mm thickness) and a slide glass (76 mm
× 26 mm, S-0314 Neo no. 2, 1.0–1.2 mm thickness) were
purchased from Matsunami Glass Industries (Osaka, Japan). 1-Ethyl-3-[3-dimethylaminopropyl]carbodiimide
(EDC) was purchased from Tokyo Chemical Industry (Tokyo, Japan). N-Hydroxysuccinimide (NHS) was purchased from Wako Pure
Chemical Industries (Osaka, Japan). All of the other reagents were
purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan),
unless otherwise specified, and used without further purification.
Type 1 ultrapure water (Milli-Q) was used in all of the experiments.
6
Conjugation of DSPE-PEG-NH2 Polymer
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DSPE-PEG-NH2 (with a molecular weight (MW) of 2850 g/mol and 95% purity by GPC) was purchased from Nanosoft Polymers. Inc. (Winston Salem, NC, USA). 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC, >98.0% purity) was obtained from the Tokyo Chemical Industry (Tokyo, Japan). N-hydroxy succinimide (NHS, 98% purity), di-methyl formamide (DMF, 99.8% purity) (anhydrous), 4-dimethylaminopyridine (DMAP, ≥99% purity), and TMA (95%) were purchased from Sigma-Aldrich (St. Louis, MO, USA).
7
Polycaprolactone-Chitosan-Gallic Acid Scaffold
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Polycaprolactone (Mn. 80 kDa), chitosan (medium molecular weight, 200–800 cP), and gallic acid (GA) were purchased from Sigma-Aldrich (Milwaukee, WI, USA). N-hydroxysuccinimide (NHS) and 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) were purchased from Tokyo Chemical Industry Co. Ltd. (TCI-SU, Tokyo, Japan). Ginsenoside Compound K (CK) from the roots of Panax ginseng C.A. Mey was purchased from ChemFaces (Wuhan, China). Chloroform and methyl alcohol were purchased from Samchun Pure Chemicals (Pyeongtaek, South Korea). All the other chemicals were of analytical grade.
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