Testosterone d3

Manufactured by Merck Group

Sourced in United States, Italy

Testosterone-d3 is a stable isotope-labeled compound used as an internal standard in analytical methods for the quantification of testosterone in biological samples. It serves as a reference substance to ensure accurate and reliable measurement of testosterone levels.

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Lab products found in correlation

Gibberellic acid, by Merck Group (9 mentions) Jasmonic acid, by Merck Group (9 mentions) Thymine d4, by Merck Group (9 mentions) Glutamic acid d5, by Merck Group (8 mentions) 15n anthranilic acid, by Merck Group (8 mentions) Trans zeatin, by Merck Group (6 mentions) Sarcosine d3, by Merck Group (5 mentions) N acetyl aspartic acid d3, by Merck Group (5 mentions) Tryptophan 15n2, by Merck Group (5 mentions) Methanol, by Avantor (4 mentions) Formic acid, by Merck Group (4 mentions) Ammonium acetate, by Merck Group (4 mentions) 15n 2 tryptophan, by Merck Group (3 mentions) Acetonitrile, by Merck Group (3 mentions) Hplc grade acetonitrile, by Avantor (2 mentions) Acetonitrile, by Thermo Fisher Scientific (2 mentions) Chloroform, by Avantor (2 mentions) Ms grade formic acid, by Merck Group (2 mentions) Hplc grade ammonium acetate, by Avantor (2 mentions) Trans zeatine, by Merck Group (2 mentions)

Close spelling variants of this product

Testosterone (291 citations)

20 protocols using testosterone d3

HPLC-MS Chemical Standards Protocol

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High-performance liquid chromatography (HPLC) grade acetonitrile, methanol, and water were purchased from Burdick & Jackson (Morristown, NJ). The mass spectrometry grade formic acid and internal standards, including N-acetyl L-aspartic acid-d3, L-tryptophan-¹⁵N₂, sarcosine-d₃, glutamic acid-d₅, thymine-d₄, gibberellic acid, trans-zeatin, jasmonic acid, 15N anthranilic acid, and testosterone-d₃, were purchased from Sigma-Aldrich (St.Louis, MO). The ESI-L Low Concentration Tuning Mix (Agilent Technologies, Santa Clara, CA) was used to calibrate the mass spectrometer.

Jiao L., Maity S., Coarfa C., Rajapakshe K., Chen L., Jin F., Putluri V., Tinker L.F., Mo Q., Chen F., Sen S., Sangi-Hyghpeykar H., El-Serag H.B, & Putluri N. (2019). A prospective targeted serum metabolomics study of pancreatic cancer in postmenopausal women. Cancer prevention research (Philadelphia, Pa.), 12(4), 237-246.

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Quantitative Analysis of Steroid Hormones

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We purchased etonogestrel, levonorgestrel, medroxy progesterone acetate, norethindrone, progesterone, testosterone-d3, estradiol, ethinyl estradiol, dansyl chloride, sodium bicarbonate, ammonium acetate and formic acid from Sigma-Aldrich. estradiol-d5 was purchased from CDN Isotopes. progesterone and estradiol stock solutions were purchased from Abbott Laboratories. Water, methanol, acetonitrile, N-butylchloride, and sodium hydroxide were purchased from Fisher Scientific. We purchased double-stripped human serum from Golden West Biologicals.

Zhang J., Tang C., Oberly P.J., Minnigh M.B., Achilles S.L, & Poloyac S.M. (2019). A Sensitive and Robust UPLC-MS/MS Method for Quantitation of Estrogens and Progestogens in Human Serum. Contraception, 99(4), 244-250.

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Steroid Quantification by LC/MS

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Optima LC/MS grade methanol, acetonitrile, water, and formic acid were purchased from Fisher Scientific (Pittsburgh, PA). Sulfatase (Type H-1, from Helix pomatia) was purchased from Sigma-Aldrich (St. Louis, MO). Steroid standards DHEA, E2, T, DHEA-d5, estradiol-d5, and testosterone-d3 were purchased from Sigma-Aldrich. Steroid standards were prepared on the day of use.

Yoo H.S, & Napoli J.L. (2019). Quantification of Dehydroepiandrosterone, 17β-Estradiol, Testosterone, and Their Sulfates in Mouse Tissues by LC-MS/MS. Analytical chemistry, 91(22), 14624-14630.

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Steroid Hormone Analytical Protocols

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The following were purchased from Sigma-Aldrich (St. Louis, MO, USA): the steroid hormone corticosterone and its precursors including pregnenolone, progesterone, 11-deoxycorticosterone, dehydrocorticosterone, testosterone and its precursor, 17-hydroxyprogesterone, androstenedione and dihydrotestosterone; and stable isotopes, including testosterone-d3. The stable isotope corticosterone-d8 was purchased from Otsuka Pharmaceutical (Tokyo, Japan). LC-MS-grade acetonitrile and formic acid were purchased from Supelco (Bellefonte, PA, USA).

Maeda N., Tahata S., Yagi T., Tanaka E., Masu K., Sato M., Haeno S., Onaga T, & Yokota H. (2015). Assessment of Testicular Corticosterone Biosynthesis in Adult Male Rats. PLoS ONE, 10(2), e0117795.

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Comprehensive Metabolite Analysis by HPLC-MS

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High-performance liquid chromatography (HPLC)-grade ammonium acetate from Sigma, acetonitrile, methanol, chloroform, and water were procured from Burdick & Jackson (Morristown, NJ). Mass spectrometry-grade formic acid was purchased from Sigma-Aldrich (St Louis, MO).) Metabolite standards and internal standards, including N-acetyl Aspartic acid-d3, tryptophan-15N2, sarcosine-d3, glutamic acid-d5, thymine-d4, gibberellic acid, trans-zeatin, jasmonic acid, 15N anthranilic acid, and Testosterone-d3, were purchased from Sigma-Aldrich (St. Louis, MO).

Amara C.S., Ambati C.R., Vantaku V., Piyarathna D.W., Donepudi S.R., Ravi S.S., Arnold J.M., Putluri V., Chatta G., Guru K.A., Badr H., Terris M.K., Bollag R.J., Sreekumar A., Apolo A.B, & Putluri N. (2019). Serum metabolic profiling identified a distinct metabolic signature in Bladder Cancer Smokers: A key metabolic enzymes associated with patient survival. Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, 28(4), 770-781.

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Metabolite Extraction and Quantification

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HPLC grade ammonium acetate, acetonitrile, methanol, chloroform, ethanol, hexane, and water were procured from Burdick & Jackson (Morristown, NJ). MS grade formic acid, standard compounds and isotope labeled standards, including N-acetyl aspartic acid-d3, tryptophan-15N2, sarcosine-d3, glutamic acid-d5, thymine-d4, gibberellic acid, trans-zeatin, jasmonic acid, 15N anthranilic acid, and testosterone-d3, were purchased from Sigma-Aldrich (St. Louis, MO).

Ambati C.R., Vantaku V., Donepudi S.R., Amara C.S., Ravi S.S., Mandalapu A., Perla M., Putluri V., Sreekumar A, & Putluri N. (2018). Measurement of methylated metabolites using Liquid Chromatography-Mass Spectrometry and its biological application. Analytical methods : advancing methods and applications, 11(1), 49-57.

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Comprehensive Metabolomics Analytical Protocol

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Sources for reagents were: HPLC-grade acetonitrile and water (Burdick & Jackson); mass spectrometry-grade formic acid and ammonium acetate (Sigma-Aldrich); calibration solution containing multiple calibrants in a solution of acetonitrile, trifluroacetic acid, and water (Agilent Technologies); metabolite standards and internal standards, including N-acetyl Aspartic acid-d3, Tryptophan-15N2, Sarcosine-d3, Glutamic acid-d5, Thymine-d4, Gibberellic acid, Trans-Zeatine, Jasmonic acid, 15N Anthranilic acid, and Testosterone-d3 (Sigma-Aldrich).

Amobi-McCloud A., Muthuswamy R., Battaglia S., Yu H., Liu T., Wang J., Putluri V., Singh P.K., Qian F., Huang R.Y., Putluri N., Tsuji T., Lugade A.A., Liu S, & Odunsi K. (2021). IDO1 Expression in Ovarian Cancer Induces PD-1 in T Cells via Aryl Hydrocarbon Receptor Activation. Frontiers in Immunology, 12, 678999.

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Quantitative Metabolomics using HPLC-MS

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Acetonitrile, methanol, and water for high-performance liquid chromatography were purchased from Burdick and Jackson. Formic acid and internal standards, Gibberellic acid, Jasmonic acid, [¹⁵N]2-Tryptophan, Thymine-d4, [¹³C]-Creatinine, [¹⁵N] Arginine, [¹⁵N]-Anthranilic acid, and Testosterone-d3 (Supplementary Table 3), were purchased from Sigma Aldrich. U¹³6 glucose was used purchased from Cambridge isotope laboratories, 5-Azacytidine, Adox, 680C91 were purchased from Sigma and GSK126 was procured from Cayman chemicals.

Vantaku V., Putluri V., Bader D.A., Maity S., Ma J., Arnold J.M., Rajapakshe K., Donepudi S.R., von Rundstedt F.C., Devarakonda V., Dubrulle J., Karanam B., McGuire S.E., Stossi F., Jain A.K., Coarfa C., Cao Q., Sikora A.G., Villanueva H., Kavuri S.M., Lotan Y., Sreekumar A, & Putluri N. (2019). Epigenetic loss of AOX1 expression via EZH2 leads to metabolic deregulations and promotes bladder cancer progression. Oncogene, 39(40), 6265-6285.

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HPLC-MS Metabolite Quantification Protocol

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High-performance liquid chromatography (HPLC) grade acetonitrile, methanol and water were purchased from Burdick & Jackson (Morristown, NJ). Mass spectrometry grade formic acid and internal standards namely, Tryptophan-15N2, Glutamic acid-d5, Thymine-d4, Gibberellic acid, Trans-Zeatin, Jasmonic acid, Anthranilic acid, and Testosterone-d3 were purchased from Sigma- Aldrich (St.Louis, MO). The calibration solution containing multiple calibrants in acetonitrile/trifluroacetic acid /water was purchased from Agilent Technologies (Santa Clara, CA).

Wangler M.F., Chao Y.H., Bayat V., Giagtzoglou N., Shinde A.B., Putluri N., Coarfa C., Donti T., Graham B.H., Faust J.E., McNew J.A., Moser A., Sardiello M., Baes M, & Bellen H.J. (2017). Peroxisomal biogenesis is genetically and biochemically linked to carbohydrate metabolism in Drosophila and mouse. PLoS Genetics, 13(6), e1006825.

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Quantification of Inhaled Drugs

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Testosterone, testosterone-d₃ and fluorescein isothiocyanate dextran, average molecular weight 4000 Da (FD4) were purchased from Sigma Aldrich (Sigma Aldrich, Gillingham, UK) with ≥ 98% purity. Also purchased from Sigma Aldrich were HPLC-grade methanol. Ipratropium bromide monohydrate, tiotropium bromide monohydrate, budesonide and salbutamol sulfate were kindly supplied by GlaxoSmithKline, Stevenage, UK). Deionised water was used throughout.

Lee D.F., Lethem M.I, & Lansley A.B. (2021). A comparison of three mucus-secreting airway cell lines (Calu-3, SPOC1 and UNCN3T) for use as biopharmaceutical models of the nose and lung. European Journal of Pharmaceutics and Biopharmaceutics, 167, 159-174.

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