The cells were harvested following a brief exposure to 0.25% trypsin and 1 mM ethylenediaminetetraacetic acid (EDTA; Nacalai Tesque, Inc.). The cells were washed with 0.1% bovine serum albumin (BSA) in phosphate-buffered saline (PBS) and treated with primary mAbs for 30 min at 4 °C. Thereafter, the cells were treated with Alexa Fluor 488-conjugated anti-mouse IgG (1:2000; Cell Signaling Technology, Inc., Danvers, MA, USA) or Oregon Green anti-rat IgG (1:2000; Thermo Fisher Scientific Inc.). Then, fluorescence data were collected using the SA3800 Cell Analyzer (Sony Corp., Tokyo, Japan).
Oregon green anti rat igg
Manufactured by Thermo Fisher Scientific
Sourced in United States
Oregon Green anti-rat IgG is a fluorescent-labeled antibody that specifically binds to rat immunoglobulin G (IgG). It can be used for various immunochemical techniques, such as immunofluorescence microscopy, flow cytometry, and Western blotting, to detect and visualize rat IgG in biological samples.
Automatically generated - may contain errors
4 protocols using oregon green anti rat igg
1
Cell Staining with Fluorescent Antibodies
2
Cell Surface Marker Labeling Protocol
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The cells were harvested following a brief exposure to 0.25% trypsin and 1 mM ethylenediaminetetraacetic acid (EDTA; Nacalai Tesque, Inc.). The cells were washed with 0.1% bovine serum albumin (BSA) in phosphate-buffered saline (PBS) and treated with primary mAbs for 30 min at 4 °C. Thereafter, the cells were treated with Alexa Fluor 488-conjugated anti-mouse IgG (1:2000; Cell Signaling Technology, Inc., Danvers, MA, USA) or Oregon Green anti-rat IgG (1:2000; Thermo Fisher Scientific Inc.). Then, fluorescence data were collected using the SA3800 Cell Analyzer (Sony Corp., Tokyo, Japan).
3
Cell Harvesting and Fluorescence Analysis
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The cells were harvested following a brief exposure to 0.25% trypsin and 1 mM ethylendiaminetetraacetic acid (EDTA; Nacalai Tesque, Inc.). The cells were washed with 0.1% bovine serum albumin (BSA) in phosphate-buffered saline (PBS) and treated with primary mAbs for 30 min at 4 °C. Thereafter, the cells were treated with Alexa Fluor 488-conjugated anti-mouse IgG (1:2000; Cell Signaling Technology, Inc., Danvers, MA, USA) or Oregon Green anti-rat IgG (1:2000; Thermo Fisher Scientific Inc.). Then, fluorescence data were collected using the SA3800 Cell Analyzers (Sony Corp., Tokyo, Japan).
4
Cell Surface Protein Labeling
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The cells were harvested following brief exposure to 0.25% trypsin/1 mM EDTA (Nacalai Tesque, Inc.). The cells were washed with 0.1% BSA/PBS and treated with primary mAbs for 30 min at 4 °C. Thereafter, the cells were treated with Alexa Fluor 488-conjugated anti-mouse IgG (1:2000; Cell Signaling Technology, Inc., Danvers, MA, USA) or Oregon green anti-rat IgG (1:2000; Thermo Fisher Scientific Inc.). Fluorescence data were collected using SA3800 Cell Analyzers (Sony Corp., Tokyo, Japan).
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