Western blot analysis was carried out as previously described [29 (link)]. Lysates were fractionated by SDS-PAGE and proteins were blotted on nitrocellulose membranes. Blots were pre-blocked in PBS containing 5% (w/v) dried no fat milk and then incubated overnight at 4 °C with antibodies to anti-FASN (Sigma-Aldrich, St. Louis, MO, USA), anti-DHCR24 (Cell Signaling Technology, Danvers, MA, USA), p27 kip1 (BD Biosciences, Franklin Lakes, NJ, USA) and Bim (Abcam, Cambridge, UK). Anti-actin (Sigma-Aldrich, St. Louis, MO, USA) or anti-β-tubulin (Abcam, Cambridge, UK) antibodies were used as control for loading. Antibody binding to blots was detected by chemo-luminescence (GE Healthcare, Chicago, IL, USA). Secondary antibodies were obtained from GE Healthcare. At least three independent experiments were performed. Band intensity quantification was performed using the ImageJ 1.47 v software.
Anti dhcr24
Manufactured by Cell Signaling Technology
Sourced in United States
Anti-DHCR24 is a primary antibody produced by Cell Signaling Technology. It is designed to detect the DHCR24 (24-dehydrocholesterol reductase) protein, which is involved in the final step of cholesterol biosynthesis.
Automatically generated - may contain errors
2 protocols using anti dhcr24
1
Western Blot Analysis of Lipogenic Proteins
2
Blood Lipid Test and Adenovirus Experiment
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The blood lipid test kit was purchased from Nanjing Jiancheng Biotechnology Research Institute (Nanjing, China). Eosin, and haematoxylin dye was purchased from Nanjing Chemical Reagent Factory (Nanjing, China).
The recombinant adenovirus Ad-DHCR24 was purchased from Obio Technology (Shanghai) Corp., Ltd. Antibodies used in the experiment, such as anti DHCR24, Akt, chop and bid were purchased from cell signaling technology company (Danvers, Massachusetts, USA). Secondary antibodies such as anti-b-actin Rabbit mAb, and HRP-labeled Goat Anti-Rabbit IgG were obtained from Beijing Zhongshan Jinqiao Biotechnology Co., Ltd.
(Beijing, China).
The recombinant adenovirus Ad-DHCR24 was purchased from Obio Technology (Shanghai) Corp., Ltd. Antibodies used in the experiment, such as anti DHCR24, Akt, chop and bid were purchased from cell signaling technology company (Danvers, Massachusetts, USA). Secondary antibodies such as anti-b-actin Rabbit mAb, and HRP-labeled Goat Anti-Rabbit IgG were obtained from Beijing Zhongshan Jinqiao Biotechnology Co., Ltd.
(Beijing, China).
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