Alexa fluor 488 phalloidin

Manufactured by Vector Laboratories

Sourced in United States

Alexa Fluor 488 Phalloidin is a fluorescent dye used for the selective labeling of F-actin in cells. It is a conjugate of the natural toxin phalloidin and the Alexa Fluor 488 fluorophore. Alexa Fluor 488 Phalloidin binds tightly to F-actin, allowing for the visualization and analysis of the actin cytoskeleton in fixed cells using fluorescence microscopy.

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Lab products found in correlation

Alexa fluor 546 conjugated goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions) A1r hd25, by Nikon (1 mentions) Alexa fluor 647 conjugated goat anti mouse igg, by Thermo Fisher Scientific (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Vector Laboratories (1 mentions) Pecam 1 mec13.3, by BD (1 mentions) Cd68 fa 11, by Bio-Rad (1 mentions) Mmp 9 af909, by R&D Systems (1 mentions) Vwf a0082, by Agilent Technologies (1 mentions) P h3 ser 10, by Cell Signaling Technology (1 mentions) Vectashield mounting media with dapi, by Vector Laboratories (1 mentions)

2 protocols using alexa fluor 488 phalloidin

Visualizing DMPK Kinase Activity in MCF-7 Cells

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MCF-7 cells were transfected with the expression plasmid for HA-DMPK-wild-type (WT) or HA-DMPK-kinase dead mutant (MT). The cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100, and then blocked with 2% BSA in phosphate buffered saline (PBS), the cells were incubated with anti-HA and anti-pMLC2 antibodies. Alexa Fluor 546-conjugated goat anti-rabbit IgG (Molecular Probes, Carlsbad, CA, USA) and Alexa Fluor 647-conjugated goat anti-mouse IgG (Molecular Probes, Carlsbad, CA) were used as secondary antibodies. Alexa Fluor 488 Phalloidin and DAPI (Vector Laboratories, Inc., Burlingame, CA, USA) were used to stain actin filaments and nuclei, respectively. Images were acquired using a confocal microscope (A1R HD25, Nikon) and then analyzed with ImageJ software (NIH).

Itoh K., Ebata T., Hirata H., Torii T., Sugimoto W., Onodera K., Nakajima W., Uehara I., Okuzaki D., Yamauchi S., Budirahardja Y., Nishikata T., Tanaka N, & Kawauchi K. (2019). DMPK is a New Candidate Mediator of Tumor Suppressor p53-Dependent Cell Death. Molecules, 24(17), 3175.

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Liver Histology and Immunohistochemistry Protocol

Cited 1 time

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Liver histology and immunohistochemistry were performed as previously published.[14 (link), 15 (link)] Liver specimens embedded in paraffin were processed for hematoxylin and eosin (H&E) staining. Liver steatosis was assessed by Oil Red O staining. Immunohistochemistry was performed using CD3 (HIT3a), Ly-6G (1A8), and PECAM-1 (MEC13.3) from BD Biosciences, CD68 (FA-11, Serotec), MMP-9 (AF909; R&D Systems), vWF (A0082, DAKO), PCNA (PC10; Neomarkers) and pH3 (Ser10; Cell Signaling) antibodies at optimal dilutions. Dual/triple staining was detected by immunofluorescence with Alexa Fluor 594 (red) and Alexa Fluor 488 (green) labeled secondary antibodies (Molecular Probes). Alexa Fluor 488 phalloidin and Vectashield mounting media with DAPI (Vector Laboratories) were used for F-actin and nuclear staining, respectively. Sections were blindly evaluated by counting 10 high-powered fields (HPFs)/section in triplicate. The proliferation index is expressed as the percentage of PCNA, or pH3 stained hepatocytes per total number of hepatocytes.

Kato H., Kuriyama N., Duarte S., Clavien P.A., Busuttil R.W, & Coito A.J. (2014). MMP-9 DEFICIENCY SHELTERS ENDOTHELIAL PECAM-1 EXPRESSION AND ENHANCES REGENERATION OF STEATOTIC LIVERS AFTER ISCHEMIA AND REPERFUSION INJURY. Journal of hepatology, 60(5), 1032-1039.

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