Kc gro

Manufactured by Mesoscale

KC-Gro is a laboratory equipment product designed for cultivating microorganisms. It provides a controlled environment for the growth and maintenance of various microbial cultures. The core function of KC-Gro is to facilitate the cultivation of microorganisms under specific temperature, humidity, and lighting conditions.

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Lab products found in correlation

Sonicator, by Emerson (1 mentions) Tissue homogenizer, by Qiagen (1 mentions) Ripa buffer, by Boston BioProducts (1 mentions) Phosstop, by Roche (1 mentions) Mip2 α, by R&D Systems (1 mentions) M csf, by Thermo Fisher Scientific (1 mentions) Rankl, by Thermo Fisher Scientific (1 mentions) Il 1β, by Mesoscale (1 mentions) Interleukin 2 (il 2), by Mesoscale (1 mentions) Ifn γ, by Mesoscale (1 mentions) Il 12p70, by Mesoscale (1 mentions) Il 10, by Mesoscale (1 mentions) Interleukin 6 (il 6), by Mesoscale (1 mentions) Tnf α, by Mesoscale (1 mentions)

3 protocols using kc gro

Cytokine Profiling of Frozen Tumors

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Frozen tumors of each group were prepared using a lysis cocktail buffer (RIPA buffer, Boston Bioproducts Inc., Boston, MA) including phosSTOP, cOmplete (Roche, Indianapolis, IN). Tumors were homogenized with a tissue homogenizer (Qiagen, Valencia, CA), and the suspensions were sonicated using a sonicator (Branson, Notredam, IN). All samples were prepared at a protein concentration of 2μg/ml. The pro-inflammatory Panel 1 Mouse V-PLEX kit was used to measure the concentration of several cytokines – IL-1β, IL-2, IL-6, IL-8, IL-12, IFN-γ, TNF-α and KC-Gro (Meso Scale Discovery, Rockville, MD).

Chen Y., Ramjiawan R.R., Reiberger T., Ng M.R., Hato T., Huang Y., Ochiai H., Kitahara S., Unan E.C., Reddy T.P., Fan C., Huang P., Bardeesy N., Zhu A.X., Jain R.K, & Duda D.G. (2015). CXCR4 inhibition in tumor microenvironment facilitates anti-PD-1 immunotherapy in sorafenib-treated HCC in mice. Hepatology (Baltimore, Md.), 61(5), 1591-1602.

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In Vitro Osteoclast Generation

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For in vitro osteoclasts generation, bone marrow cells were obtained from wildtype BALB/c mice (Harlan), and CD-11b⁺ cells were cultured with M-CSF and RANKL (both Peprotech). Levels of CXCL1 (KC-GRO, Meso Scale Discovery) and CXCL2 (MIP-2α, R&D systems) were measured in the supernatants.

Wigerblad G., Bas D.B., Fernades-Cerqueira C., Krishnamurthy A., Nandakumar K.S., Rogoz K., Kato J., Sandor K., Su J., Jimenez–Andrade J.M., Finn A., Bersellini Farinotti A., Amara K., Lundberg K., Holmdahl R., Jakobsson P.J., Malmström V., Catrina A.I., Klareskog L, & Svensson C.I. (2015). Autoantibodies to citrullinated proteins induce joint pain independent of inflammation via a chemokine-dependent mechanism. Annals of the Rheumatic Diseases, 75(4), 730-738.

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BALF Cytokine Profiling via MSD

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The BALF supernatant samples were analysed using MSD pro-inflammatory panel V-Plex including the following analytes: IFN-γ, IL-10, IL-12p70, IL-1β, IL-2, IL-4, IL-5, IL-6, KC/GRO and TNF-α (Meso Scale Diagnostics, Rockville, MD). The analysis was performed according to the manufacturer’s instruction.

Wronski S., Dannenmaier J., Schild S., Macke O., Müller L., Burmeister Y., Seilheimer B, & Müller M. (2018). Engystol reduces onset of experimental respiratory syncytial virus-induced respiratory inflammation in mice by modulating macrophage phagocytic capacity. PLoS ONE, 13(4), e0195822.

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