Goat anti mouse igg2a hrp

Manufactured by Santa Cruz Biotechnology

Goat Anti-Mouse IgG2a-HRP is a secondary antibody product that binds to the IgG2a subclass of mouse immunoglobulins and is conjugated with horseradish peroxidase (HRP). This product is intended for use in immunoassay and other immunochemical applications.

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Goat anti-mouse HRP (51 citations) IgG2a (12 citations) Mouse anti-goat (5 citations) Mouse IgG2a (3 citations) HRP-mouse (3 citations) Anti-mouse IgG2a (2 citations)

2 protocols using goat anti mouse igg2a hrp

Periplocin's Modulation of Osteoclastogenesis

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Periplocin was purchased from State General Administration of the People's Republic of China for Quality Supervision and Inspection and Quarantine. The recombinant human RANKL was purchased from Peprotech Biotechnology. M-CSF was purchased from eBioscience. STAT3, phospho-I-κBα, phospho-p65, c-Fos, cathepsin K, NFATc1, Goat Anti-Mouse IgG1-HRP and Goat Anti-Mouse IgG2a-HRP antibodies were purchased from Santa Cruz Biotechnology. I-κBα, TLR4 and MMP-9 antibodies were purchased from Abcam. Phospho-STAT3 Tyr705 antibodies was obtained from Signalway. Alfa-MEM medium, fetal bovine serum and trypsin were purchased from Gibco Inc. TNF-α, IFN-γ, IL-1β, IL-4 and IL-22 ELISA kit were purchased from ebioscience. DMSO and MTT were purchased from Sigma-Aldrich Shanghai, China.

Bao C., Liu Y., Sun X., Xing C., Zeng L, & Sun G. (2017). Periploca forrestii saponin ameliorates CIA via suppressing proinflammatory cytokines and nuclear factor kappa-B pathways. PLoS ONE, 12(5), e0176672.

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Histological Analysis of Lung Tissue

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Formalin fixed, paraffin embedded lung tissue was sectioned into 5 μm sections and stained by hematoxylin and eosin or Masson's trichrome stain. Immunohistochemistry for α-smooth muscle actin (Santa Cruz Biotechnology, clone 1A4, 1:500 dilution) was performed without antigen retrieval, goat anti-mouse IgG2a-HRP (Santa Cruz Biotechnology, 1:500 dilution) was used as a secondary antibody, with diaminobenzene (DAB; Vector Laboratories) as a substrate. Mayer's hematoxylin was used as a counterstain for immunohistochemistry. Before sectioning and staining, tissue blocks were coded, and the pictures were taken blinded. For H&E and trichrome staining, images were acquired randomly. For α-smooth muscle actin staining, areas of focal consolidation were chosen for image acquisition (also blinded) to identify areas of inflammation and/or fibrosis.

Brodeur T.Y., Robidoux T.E., Weinstein J.S., Craft J., Swain S.L, & Marshak-Rothstein A. (2015). IL-21 Promotes Pulmonary Fibrosis through the Induction of Pro-fibrotic CD8+ T Cells. Journal of immunology (Baltimore, Md. : 1950), 195(11), 5251-5260.

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