Plasma concentrations of TAG, NEFA, cholesterol, ALT and AST were measured using the following commercial kits according to the manufacture's instruction: TAG (Sigma, TR0100); NEFA (Wako Diagnostics, 993-35191); cholesterol (Stanbio, SB-1010-430); ALT (Stanbio, 2930); AST (Stanbio, 2920). For liver hydroxyproline content, liver tissue was homogenized in water and samples were hydrolyzed by incubation with 6N hydrochloric acid at 120 °C for 3 h, followed by measurement using Hydroxyproline Colorimetric Assay Kit (BioVision, K555-100). Liver TAG was extracted as previously described [32] (link).
Cholesterol
Manufactured by EKF Diagnostics
Sourced in United States
The Cholesterol lab equipment product by EKF Diagnostics is a device used to measure the levels of cholesterol in a person's blood. It provides accurate and reliable results to help healthcare professionals assess an individual's cardiovascular health.
Automatically generated - may contain errors
Lab products found in correlation
Hydroxyproline colorimetric assay kit, by Abcam (1 mentions)
Cholesterol, by Merck Group (1 mentions)
Glycerol, by Merck Group (1 mentions)
Mouse insulin elisa kit, by Crystal Chem (1 mentions)
Microvette tube, by Sarstedt (1 mentions)
Insulin, by Crystal Chem (1 mentions)
Non esterified fatty acid, by Fujifilm (1 mentions)
4 protocols using cholesterol
1
Liver Biomarker Measurement Protocol
2
Metabolic Profiling of Fasted and Fed Mice
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Fasted mice were starved overnight (15–16 hours) [62 (link)] and measurements were taken at the beginning of the light cycle. To better synchronize the feeding schedule for fed measurements, food was removed from the cages during the light cycle, and mice were fed ad libitum during the dark cycle. Fed measurements were taken at the beginning of the light cycle after three nights of entrainment as described [63 (link)]. Glucose was measured with a drop of whole blood using a glucometer (Bayer Contour, Leverkusen, Germany). Whole blood was collected from mice using Microvette® tubes (CB300Z, Sarstedt, Nümbrecht, Germany) under fasting and fed conditions. To extract serum from whole blood, the blood was allowed to clot for 30–90 min at room temperature and then centrifuged at 2000 x g for 20 min (4°C), per provided instructions from Microvette. Insulin levels were determined using a mouse insulin ELISA kit (Crystal Chem Inc, Downers Grove, IL). Serum triglycerides (Wako Diagnostics, Richmond, VA), non-esterfied fatty acids (Wako Diagnostics, Richmond, VA), cholesterol (Stanbio, Boerne, TX) and glycerol (Sigma-Aldrich, St. Louis, MO) were determined with enzymatic colorimetric assays.
3
Serum Lipid and Insulin Assay
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Serum levels of total triglyceride, cholesterol (Stanbio Laboratory, Boerne, TX, USA) and insulin (Crystal Chem, Downers Grove, IL, USA) were measured using commercial kits according to manufacturers’ instructions.
4
Hepatic Lipid Extraction and Quantification
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Lipids were extracted from liver samples using Folch solution composed of a 2:1 (vol/vol) mixture of chloroform/methanol as described previously56 (link). Lipids were solubilized in 10% Triton X-100 before evaporation. Non-esterified fatty acid (Fujifilm/Wako, Lexington, MA, USA), cholesterol (Stanbio, Boerne, TX, USA), and triglyceride content (Stanbio, Boerne, TX, USA) were measured in hepatic extracts and plasma samples using colorimetric assays.
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